Molecular based species identification is a useful tool in forensic investigations as well as routine work. The ability to cheaply and quickly determine the species of origin of a sample has become increasingly necessary as incidents of wildlife poaching, illegal meat trade and the trade of wildlife products has increased. The current methods of species identification tend to be expensive, time consuming and unreliable. The use of species-specific primers designed to bind to specific areas in the mitochondrial DNA, has been published. This application has been developed for a small number of domestic animals, however the application of these primers to African wildlife species has yet to be done. This method is relatively simple and is based on specific fragment size amplification using polymerase chain reaction and genotyping. A total of thirty seven different species were analyzed with this method and 30 of these species were found to have species-specific fragment sizes. A number of different sample types and conditions were tested including uncommon diagnostic samples such as rhinoceros horn, teeth and claws. In addition, the sensitivity of the method was investigated and determined to be very high, detecting species at a DNA concentration of just 0.1 pg/μl. This method was found to be a highly sensitive, efficient and a fast way to determine species in a number of different sample types and would therefore be of great value in the wildlife trade as these samples can often be of a lower quality or only available in small amounts. The use of this method in forensic science must be done with care due to the problem of cross species amplification. In addition not all of the African Wildlife mammals were available to test, limiting the detection power and specificity of the test. Copyright / Dissertation (MSc)--University of Pretoria, 2012. / Production Animal Studies / unrestricted

This thesis explores landscape change in one of Africa’s biggest parks from the perspectives of its managers, the international conservation community, and media in South Africa and the United States. The surprising history of Kruger's elephant population reflects the complicated relationship between shifting wildlife management approaches, environmental ethics, and understandings of African nature, which continue to influence future conservation priorities. Elephants, because of their capacity to drive ecosystem change, expose a history of conflict over what nature means in the Kruger Park and how it should be managed. Current management philosophies in the park reflect the need to prepare for an uncertain future but also to confront an unsettled inheritance of the past. I delve into the 20th century chronicles of science, landscape aesthetics, wilderness ethics, and international politics that inform conservation in Kruger today.