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Agrocin 84 selectivity and toxicity in Agrobacterium / by Peter Jeffrey MurphyMurphy, Peter Jeffrey January 1981 (has links)
Typescript (photocopy) / x, 100 leaves, [40] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology and Agricultural Biochemistry, 1982
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Agrocin 84 selectivity and toxicity in Agrobacterium /Murphy, Peter Jeffrey. January 1981 (has links) (PDF)
Thesis (Ph.D.)-- University of Adelaide, Dept. of Plant Pathology and Agricultural Biochemistry, 1982. / Typescript (photocopy).
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Molecular factors that influence host range and virulence of A. tumefaciensCastle, Linda A. 08 August 1990 (has links)
Agrobacterium tumefaciens is a common soil bacterium that causes crown gall
disease on many dicotyledonous plant species. It has been exploited for use in genetic
engineering because it is able to promote transfer of any DNA segment to plant
chromosomes. Many molecular factors in both the plant and bacterium contribute to
successful infection and DNA transfer.
Two bacterial factors were studied for this dissertation. The first was bacterial
synthesis of cytokinins due to the induced expression of the gene tzs. Cytokinin
production by some A. tumefaciens strains at the time of infection was hypothesized to
enhance infection or influence host range. To test this hypothesis, heterologous strains
engineered to express tzs and a native strain from which tzs was deleted were evaluated
for tumor induction on a number of plant species. Major differences were seen when
radish roots were infected with the tzs deletion strain. Morphological differences of the
galls formed suggested a possible role for tzs in T-DNA transfer efficiency. β-glucuronidase
assays with a mannopine synthase promoter-uidA fusion gene were
used to study T-DNA transfer early in the infection process. No biological role for tzs
was confirmed with either tumorigenesis or T-DNA transfer assays. However, optimized
conditions for GUS fluorometric assays were established and the infection process was
characterized.
The second factor studied was a β-glucosidase gene in A. tumefaciens strain
83/73. This strain is highly infective on conifer hosts, whereas others are not. It is also
able to hydrolyze the conifer phenolic compound, coniferin, to an active virulence gene
inducer, coniferyl alcohol. This appears to be a major host range determining factor.
As a first step in proving this role, the gene for the β-glucosidase enzyme responsible
for hydrolysis of coniferin in strain B3/73 was cloned and sequenced. The deduced
protein sequence has homology to other β-glucosidases, including a conserved active
site. / Graduation date: 1991
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"Agrobacterium" : plasmids and biovars /Ophel, Kathleen Margaret. January 1987 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Pathology, 1988. / Includes bibliographical references (leaves 113-130).
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Characterization of phages of the crown gall bacteria (Agrobacterium tumefaciens (Smith and Townsend) Conn.).Chen, Peter Kea-yet, January 1962 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Use of dyes in the differentiation of the Agrobacterium and Rhizobium speciesHamdi, Yousef Ali Mahmoud. January 1963 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1963. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 190-199).
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Characterization of an avirulent mutant of Agrobacterium tumefaciensHolroyd, Barbara Jane. January 1976 (has links)
Thesis--Wisconsin. / Includes bibliographical references (leaves 90-98).
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Biopelículas en Agrobacterium sppAbarca Grau, Ana María 26 September 2011 (has links)
Diversas especies del género Agrobacterium producen tumores en gran cantidad de plantas cultivadas, causando la enfermedad conocida en inglés como "crown gall". En esta tesis se ha demostrado que cepas patógenas y no patógenas de las especies A. tumefaciens, A. rhizogenes y A. vitis son capaces de formar biopelículas tanto sobre superficies inertes como sobre raíces de tomate. Las cepas de A. tumefaciens y A. vitis se unieron a poliestireno y polipropileno, mientras que las de A. rhizogenes sólo se unieron a polipropileno. Se ha constatado que la formación de biopelículas in vitro sobre superficies abióticas en Agrobacterium spp. depende de la especie (biovar), la superficie y las condiciones de cultivo. Mediante microscopía electrónica de barrido y microscopía láser confocal, utilizando cepas marcadas con GFP, se demostró que las tres especies formaban estructuras complejas compuestas por numerosas células bacterianas dispuestas según alguno de los siguientes modelos: 1) tapices densos y continuos, 2) grandes agregados irregulares embebidos en material extracelular, o 3) en forma de setas globulares atravesadas internamente por canales. Los resultados sugieren que la formación de biopelículas puede estar asociada a la colonización y supervivencia de estas especies bacterianas en la rizosfera. Además, la capacidad del agente de biocontrol A. rhizogenes K84 de formar biopelículas durante su interacción con la planta podría ser una característica relevante para el control de la enfermedad. Con el propósito de ahondar y conocer genes implicados en la formación de biopelículas en este agente de biocontrol se han utilizado dos estrategias. Mediante genética clásica, se analizó una librería de mutantes al azar de la cepa K84 y se identificaron dos mutantes afectados en la formación de biopelículas. Uno fue incapaz de unirse y formar biopelículas sobre polipropileno, pero por el contrario se unió y formó biopelículas sobre ápices radiculares. El otro mutante produjo más biopelícula que la cepa K84, tanto en superficie abiótica como en ápices radiculares. / Abarca Grau, AM. (2011). Biopelículas en Agrobacterium spp [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/11670
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Development of a strategy to induce RNA-silencing in squash against virus diseases by genetic transformationKhidr, Yehia. Unknown Date (has links) (PDF)
Hohenheim, University, Diss., 2007.
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Development of a strategy to induce RNA-silencing in squash against virus diseases by genetic transformationKhidr, Yehia, January 2007 (has links)
Hohenheim, Univ., Diss., 2007.
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