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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Perfil das proteínas de fase aguda em gatos com doença do trato urinário inferior obstrutiva

Dinallo, Heloíse Rangel January 2019 (has links)
Orientador: Priscylla Tatiana Chalfun Guimarães Okamoto / Resumo: A doença do trato urinário inferior em felinos (DTUIF) apresenta diversos fatores etiológicos, sendo a forma obstrutiva a mais grave. As proteínas de fase aguda (PFAs) são biomarcadores utilizados para avaliar processos inflamatórios sistêmicos. A Alfa-1 Glicoproteína Ácida e o Amilóide A Sérico são PFAs positivas e major, o fibrinogênio é PFA minor e a albumina é negativa em gatos. O objetivo deste estudo é determinar as concentrações séricas das proteínas de fase aguda, Amilóide A sérico, Alfa-1 Glicoproteína Ácida, fibrinogênio e albumina e utilizá-las como biomarcadores de inflamação no monitoramento do processo inflamatório de gatos com doença do trato urinário inferior obstrutiva. Foram avaliados 25 gatos, machos, sem predileção de raça e idade, divididos em dois grupos experimentais, GC - grupo controle com oito gatos hígidos e GO - grupo obstruído com 17 gatos diagnosticados com DTUIF obstrutiva. Foram coletadas amostras para determinação das PFAs, bioquímica sérica, urinálise e UP/C nos M0, M12, M24 e M48 no GO e no GC somente no primeiro momento. As determinações das PFAs foram realizadas com os kits de ELISA para SAA, Kit Cat Serum Amyloid A Elisa (LIFE-SAA-8) e AGP, Kit Cat Alpha-1-Acid Glycoprotein Elisa (LIFE-AGP-8), ambos marca: Life Diagnostics®. No M0 houve correlações positivas de SAA, AGP e fibrinogênio com ureia e creatinina e correlação negativa de albumina com hematúria, SAA e potássio. No M48, houve correlações positivas entre SAA e AGP, AGP e ureia, fi... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The Feline Lower Urinary Tract Disease (FLUTD) presents several etiologic factors, with the obstructive form representing the most severe. The acute phase proteins (APPs) are biomarkers used to evaluate systemic inflammatory processes. In cats, Alpha-1-Acid Glycoprotein and Serum Amyloid A are major positive APPs, fibrinogen is a minor APP and albumin is a negative APP. This study aims at determining the serum concentrations of acute phase proteins Serum Amyloid A, Alpha-1-Acid Glycoprotein, fibrinogen and albumin, in addition of using them as biomarkers of inflammation during the monitoring of the inflammatory processes of cats with obstructive feline lower urinary tract disease. A total of 25 male cats were recruited for the study irrespective of breed and age, and were divided into two experimental groups: the control group (CG), comprised of eight healthy cats; and the obstruction group (OG), comprised of 17 cats diagnosed with obstructive FLUTD. Samples were collected for APP analysis, serum biochemical assay, urinalysis and UP/C determination at M0, M12, M24 and M48 in the OG, and at M0 in the CG. The concentrations of the APPs were determined using commercially-available ELISA kits for SAA (Kit Cat Serum Amyloid A Elisa, LIFE-SAA-8) and AGP (Kit Cat Alpha-1-Acid Glycoprotein Elisa, LIFE-AGP-8) (Life Diagnostics®). At M0, there were positive correlations of SAA, AGP and fibrinogen with urea and creatinine, as well as negative correlations between albumin and hematuria, ... (Complete abstract click electronic access below) / Mestre

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