Spelling suggestions: "subject:"animal well"" "subject:"animal cell""
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The adaptation of anchorage-dependent cells to glutamine-free mediumMcDermott, Ruth Helen January 1991 (has links)
No description available.
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Studies on the phosphorylation and tetramerisation of βII spectrinBignone, Paola A. January 2002 (has links)
No description available.
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Mammary cell cyclic AMP : regulation of breakdown and influence on protein phosphorylationAitchison, Robert E. D. January 1987 (has links)
No description available.
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The permeability of intercellular junctions formed between animal cellsFinbow, M. E. January 1979 (has links)
No description available.
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Growth inhibitors and promoters from high concentration animal cell culturesAndrews, Arcadio Garcia de Castro January 1994 (has links)
No description available.
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Membrane and micro-sparging aerations in long-term high-density perfusion cultures of animal cellsQi, Hanshi 17 December 2001 (has links)
The profile of the inner-tubing gas pressure for a tubular membrane aeration system
was quantified. The correlations among the overall volumetric oxygen transfer coefficient
(k[subscript L]a), the inner-tubing pressure, the tubing tightness, and the gas throughput are
experimentally analyzed. A mathematical model was developed to describe the
underlying phenomena. The results established the base for comparison with other
aeration techniques.
A novel method employing in situ laser imaging technology to monitor bubbles and
cells, and analyze bubble size distributions in a micro-sparged bioreactor was developed.
The effects of bioreactor operations on bubble size distributions were determined with
following results:
1) Spargers with larger pores produced larger bubbles in most cases
2) Higher sparging rates resulted in bubble size increases up to 10%
3) Pluronic F68 shrank bubbles up to 30%. When the concentration of Pluronic F68
exceeded 1 g/L, no additional impact was observed.
4) Emulsion silicone antifoam up to 25 ppm had no impact on bubbles
5) Cell density (up to 22x10��� cells/mL) or culture age has no effect on bubble sizes
In multiple 1 5-L long-term high-density cultures of animal cells, the correlations
between sparging rate and cell damage for using 0.5 ��m and 15 ��m-pore spargers were
quantified. At cell density of 2x10��� cells/mL, sparging above 0.025 vvm using the 0.5-��m
sparger was detrimental to cells, while 0.054 vvm was detrimental for the 15-��m sparger.
A model was developed to predict the rate of cell death resulted from cell-bubble
interactions for high-density industrial animal cell cultures.
The effect of high superficial velocity of sparging gas on cells at the sparger surface
proved insignificant.
A new dissolved CO��� sensor proved to be reliable for long-term use in industrial
perfusion cell cultures. A novel method for the control of dissolved CO��� while
simultaneously maintaining DO��� and pH setpoints was developed. The continuous
control of dissolved CO���, DO��� and pH is achieved by simultaneously adjusting the total
sparging rate as well as the ratio of O���, N��� and CO��� gas contents. This control strategy
enables optimization of dissolved CO��� in industrial culture processes and allows for
improved cell growth and protein production. / Graduation date: 2002
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A kinetic study of the growth of anchorage-dependent mouse L cellsHaldankar, Raj. January 1994 (has links)
Thesis (M.S.)--Ohio University, March, 1994. / Title from PDF t.p.
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Effects of matrix and phenotype on human dermal fibroblast attachment under laminar shear stress : implications for the development of tissue-engineered heart valvesJouret, Chantal 12 1900 (has links)
No description available.
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Three-dimensional scaffolds for mammary epithelial cell growth : a thesis /Barry, Megan M. Crockett, Robert S. January 2008 (has links)
Thesis (M.S.)--California Polytechnic State University, 2008. / Major professor: Robert S. Crockett, Ph.D. "Presented to the faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree [of] Master of Science in Engineering." "May 2008." Includes bibliographical references (leaves 38-45). Also available on microfiche (1 sheet).
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Effects of imperfect mixing in suspended plant and animal cell culturesCheung, Caleb Kin Lok, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW January 2006 (has links)
A common problem observed in large-scale cell cultivation is reduced culture performance compared with small-scale processes due to the existence of concentration gradients caused by poor mixing. Small-scale simulations using microbial cell suspensions have shown that circulation of cells through concentration gradients of oxygen, pH and glucose can result in reduction of cell growth and product formation similar to the effects observed in large-scale bioreactors. This study was aimed at using scale-down studies to investigate poor mixing in large-scale bioreactors used for suspended plant and animal cell culture. Two plant cell suspensions and a hybridoma cell line were used in this work. The range of oxygen transfer coefficients achieved in the hybridoma and plant suspensions were about 50???20 h-1 and 12???6 h-1, respectively. One-vessel simulation was developed to induce fluctuations of dissolved oxygen tension in a 2-L bioreactor using intermittent sparging of air and nitrogen. The effect of dissolved oxygen fluctuations on the cells was examined by comparing the performance of the cultures with those operated at constant dissolved oxygen tension. In the hybridoma suspension culture, only slight effects on cell growth were observed at circulation times above 300 s. No effect on the specific glucose uptake rate or antibody production was observed at the circulation times tested. Analysis of gene expression for selected hypoxia-related genes also suggested that the overall effect was limited. In plant cell suspensions, the specific growth rates and biomass yields on total sugar in the cultures under fluctuating dissolved oxygen tension were essentially the same as those at constant dissolved oxygen tension for both transgenic Nicotiana tabacum and Thalictrum minus. Under fluctuating dissolved oxygen tension, no effect on antibody accumulation was observed in transgenic N. tabacum suspensions, but a decrease in berberine accumulation was observed in T. minus. From the results, it can be concluded that only minimal effects due to the development of concentration gradients would be expected in large-scale bioreactors used for the cultivation of the hybridoma and plant cell suspensions tested in this work.
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