Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis(M.A.)--Case Western Reserve University, 2010 / Title from PDF (viewed on 2010-01-28) Department of Communication Sciences Includes abstract Includes bibliographical references and appendices Available online via the OhioLINK ETD Center

Thesis (Ph.D.)--University of Wisconsin--Madison, 2000. / Includes bibliographical references (p. 195-207). Also available on Internet.

Present within the biodiverse hypersaline environment are a wide variety of halotolerant filamentous fungi. Many of these phytopathogens are capable of hydrolysing plant cell wall polysaccharides such as hemicellulose which are comprised of mannans and heteromannans which are polymers of the mannose sugars. Endoacting hydrolytic enzymes such as endo-β-1,4-mannanases are secreted into the extracellular environment and are involved in the catalysis of the random hydrolysis of β-1,4-mannosidic linkages within the backbone of mannan, galactomannan, glucomannan, and galactoglucomannan. Poultry are monogastric animals that are unable to efficiently digest high-fibre and mannan rich feeds such as soybean meals and this results in decreased or depressed animal performance. The use of feeds supplemented with β-mannanases has been shown to enhance the feeding value of mannan-based meals. In the current study, the degradation of β-mannan polysaccharides present in poultry feed by halotolerant Scopulariopsis candida LMK008 β-mannanase was investigated. SDS-PAGE, Native-PAGE in conjunction with zymogram analysis was used to assess the molecular weight of the endomannanases. At least three isozymes were detected: two of 56 kDa (pI 3.5 and 6.7) and one of 28 kDa. Anion exchange chromatography was used to purify the 28 kDa isozyme. Three mannan-based substrates, viz., locust bean gum, guar gum and soybean flour, were used to evaluate the hydrolysis capability of the crude as well as the purified β-mannanase via the release of reducing sugars and was detected using the DNS assay. The β-mannanase exhibited low activity with pure guar gum but high activity with locust bean gum galactomannan and soybean flour mannan. The hydrolysis activities of the crude and purified enzyme were then tested further on mannan-based soybean meals. In general it was found that more reducing sugars were released from the grower feed than the starter and layer feeds. Another common hydrolysis pattern observed in all feed types was that after prolonged incubation of 24 h there was a decrease in the amount of reducing sugars released which could be attributed to the presence of naturally-occurring microorganisms in the feed sample which metabolised the simple sugars resulting from the enzymatic hydrolysis of the mannan components in the feed samples. This was confirmed by standard plate count assays. The results obtained are encouraging and the purified β-mannanase could be applied as an industrial feed additive within the animal feed industry, however, further testing of the enzyme in situ is needed in order to prove its applicability. The cloning of the endomannanase has to date proven unsuccessful despite numerous techniques being employed and further research is also needed to accomplish this task. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2012.

Thesis (M.S.)--University of Texas at El Paso, 2007. / Title from title screen. Vita. CD-ROM. Includes bibliographical references. Also available online.

Thesis (Ph.D.)--University of Wisconsin--Madison, 2000. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (p. 195-207).

The chemical analysis of feed samples can be time consuming and expensive. The use of near infrared reflectance (NIR) spectroscopy was evaluated in a range of studies as a rapid technique to predict the chemical constituents in feedstuffs and compound ostrich feeds. The prediction of accurate results by NIR spectroscopy relies heavily upon obtaining a calibration set which represents the variation in the main population, accurate laboratory analyses and the application of the best mathematical procedures. This research project was designed to meet five objectives: The first objective was to determine the feasibility of using near infrared reflectance (NIR) spectroscopy to predict dry matter, ash, crude protein, crude fibre, oil content, and fatty acids such as palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2) in sunflower seed meal. The second objective was to develop calibration models to predict the dry matter, crude protein and oil content in milled canola seed, compared to whole canola seeds. The third objective was to investigate the feasibility of using NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre and oil content in milled lupin seeds, compared to whole lupin seeds. The fourth objective was to describe the development of near infrared reflectance (NIR) spectroscopy calibration equations for the prediction of chemical composition and amino acid content from different populations of alfalfa hay (Medicago sativa L.). The last objective was to determine the potential of NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre, ether extract, acid detergent fibre (ADF), neutral detergent fibre (NDF), calcium, phosphorus, in vitro organic matter digestibility (IVOMD) and amino acids such as lysine, methionine, threonine and arginine in compound ostrich feed samples. The results of this study indicate that NIR spectroscopy calibrations in sunflower seed meal are only applicable in sunflower breeding programmes for a fast screening as it was not suitable for prediction purposes. Screening of sunflower seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that is a great utility for users who need to analyse a large number of samples. Calibrations developed for crude protein and oil content in milled canola seeds proved to be better than calibrations for whole canola seeds. Although the results indicated that calibrations were better for milled canola seeds, it indicated values that were typical of equations suitable for screening purposes to select samples for more detailed chemical analysis. According to calibration statistics obtained for crude protein, crude fibre and oil content in whole lupin seeds, there is no need to grind the seeds to scan the meal as similarly accurate results were obtained by analysing whole seeds. Screening of whole lupin seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that may be of great utility for users who need to analyse a large number of samples with no sample preparation. The calibration and validation statistics obtained in the study to test the potential of NIR spectroscopy to predict the chemical composition and amino acid contents in alfalfa hay, showed the accuracy was too low for routine analysis, although NIR spectroscopy could be used as a screening tool. Further research needs to be done to improve the accuracy of the NIR spectroscopy analysis, including more samples from different cultivars and years. In the study to examine the possibility of using NIR spectroscopy to predict the chemical composition of compound ostrich feeds, the results indicated that NIR spectroscopy is a suitable tool for a rapid and reliable prediction of the crude protein, crude fibre, ether extract, IVOMD, ADF and NDF in compound ostrich feeds. Calibrations can be improved for amino acids if a larger sample pool is used to develop the calibrations. These studies indicated that NIR spectroscopy can be a rapid and successful tool for the prediction of the nutritive value up to certain amino acid contents of feedstuffs and compound ostrich feeds.

Call number: LD2668 .T4 1958 O65

Call number: LD2668 .T4 1959 L66

Thesis (Ph.D.) --University of Limpopo, 2012 / A series of experiments were carried out to evaluate the feeding values of forage sorghum (Sorghum sudanense), cowpea (Vigna ungiculata), lablab (Lablab purpureus) and mucuna (Mucuna pruriens) hays for Pedi goat production in Limpopo Province, South Africa. The first study determined nutrient composition and tannin contents of the forages. The experimental design was a completely randomised design with four treatments: forage sorghum, cowpea, lablab and mucuna hays. All the legume species had higher (P<0.05) protein contents than sorghum hay, ranging from 18 to 22 %. Within the legume species, cowpea hay had a higher (P<0.05) protein content than lablab and mucuna hays. Similarly, lablab hay had a higher (P<0.05) protein content than mucuna hay. Mucuna hay had a higher (P<0.05) concentration of both condensed tannins and hydrolysable tannins than cowpea, lablab and sorghum hays, while lablab hay had the highest (P<0.05) concentration of total polyphenols. The second study determined relative palatability indices of Pedi goats offered forage sorghum, cowpea, lablab and mucuna hays. Four male Pedi goats aged five months and weighing an average of 18 + 2 kg were used. The experimental design was a completely randomized design, with feeds (forage sorghum, cowpea, lablab and mucuna) as treatments and individual animals as replicates. Lablab and mucuna legumes had higher (P<0.05) intake and palatability indices than sorghum and cowpea hays. Palatability indices were positively and significantly (P<0.05) predicted from dry matter intakes of goats (r2 = 0.98). However, there were poor and non-significant (P>0.05) relationships between nutrient contents of the forages and their intake and palatability indices by goats. The third study determined diet intake, digestibility and live weight gain of seven months old Pedi goats fed a basal diet of forage sorghum supplemented with different amounts of cowpea, lablab or mucuna hays. The experimental design was a completely randomized design, with individual animals placed in digestibility crates as experimental units. This study was divided into a series of experiments due to lack of equipment to conduct the whole experiment in one session. A total of four experiments were conducted. Fifteen growing male Pedi goats were used in each experiment, with mean live weights of 15 + 4 kg, 18 + 2 kg, 17 + 3 kg and 20 + 4 kg for experiments 5.1, 5.2, 5.3 and 5.4, respectively. In each experiment, different goats were used. Experiments 5.1 to 5.3 involved cowpea, lablab and mucuna hays, respectively, while Experiment 5.4 compared the levels of supplementation for optimum intake from each of the first three experiments. Dry matter intakes of cowpea and lablab hay were optimised at 39 and 32 %, respectively, while that of mucuna hay increased with increasing levels of mucuna hay supplementation. Dry matter digestibilities of cowpea, lablab and mucuna hays increased with increasing levels of the respective forage supplementations. Cowpea and mucuna hay supplementations improved (P<0.05) final live weights and feed conversion ratios, while goats on lablab hay supplementation lost weight. At optimum intake, goats supplemented with mucuna hay had a better (P<0.05) feed conversion ratio than those supplemented with lablab and cowpea hays. The fourth study determined in vitro gas production of pure and mixtures of forage sorghum with cowpea, lablab and mucuna hays. Two experiments were conducted. In the first experiment (Experiment 6.1) the experimental design was a completely randomised design with four treatments (forage sorghum, cowpea, lablab and mucuna). The legume species of cowpea, lablab and mucuna hays produced more (P<0.05) gas than sorghum hay after 12 hours of incubation. Sorghum hay produced more (P<0.05) gas than cowpea, lablab and sorghum hays after 24, 48 and 72 hours of incubation. Sorghum hay dry matter intake by goats in the palatability trial was positively and significantly (P<0.05) predicted from gas production after 12 (r2 = 0.99), 24 (r2 = 0.97) and 48 (r2 = 0.93) hours of incubation. Cowpea hay, lablab hay and mucuna hay dry matter intakes by goats were poorly predicted from gas production after 12, 24, 48 and 72 hours of incubation. In the second experiment (Experiment 6.2), the experimental design was a completely randomised design with three treatments (mixtures at optimum intake): 1. FS61C39: A mixture of 61 % forage sorghum and 39 % cowpea, 2. FS68L32: A mixture of 68 % forage sorghum and 32 % lablab, 3. FS77M23: A mixture of 77 % forage sorghum and 23 % mucuna. There were no differences in gas production (P>0.05) across dietary mixtures after 12, 24, 48 and 72 hours of incubation. Dry matter intake by Pedi goats of sorghum hay mixed with cowpea at 39 % hay was positively and significantly (P<0.05) predicted from gas production of the mixtures after 24 hours (r2 = 1.0) of incubation. Dry matter intake from gas production from the same mixture was poorly predicted from gas production after 48 (r2 = 0.45) and 72 (r2 = 0.13) hours of incubation. Dry matter intake of sorghum hay by Pedi goats supplemented with lablab hay at 32 % was moderately predicted from gas production after 48 (r2 = 0.67) and 72 (r2 = 0.60) hours, but poorly predicted after 12 (r2 = 0.50), 24 (r2 = 0.53) hours of incubation. Dry matter intake of sorghum hay by Pedi goats supplemented with 23 % mucuna hay was positively predicted from gas production after 12 (r2 = 0.90) hours, but moderately predicted after 24 (r2 = 0.80), 48 (r2 = 0.72) and 72 (r2 = 0.83) hours of incubation. Dry matter digestibility of sorghum hay mixed with 23 % mucuna hay was positively and significantly (P<0.05) predicted from gas production after 24 (r2 = 1.0), 48 (r2 = 0.99) and 72 (r2 = 1.0) hours of incubation. Dry matter digestibility of sorghum hay mixed with 39 % cowpea hay was positively predicted from gas production after 48 (r2 = 0.95), moderately predicted after 24 and 72, and poorly predicted after 12 hours of incubation. Dry matter digestibility of sorghum hay mixed with 32 % lablab hay was positively predicted from gas production after 72 (r2 = 0.92) hours of incubation. The relationships between digestibility of the mixtures and gas production after 12, 24 and 48 hours of incubation were poor. The fifth study determined in sacco degradation of mixtures at optimum intake of forage sorghum mixed with cowpea, lablab or mucuna when incubated in goats. The ‘a’ values and dry matter losses in cowpea and lablab hays were higher (P < 0.05) than those of mucuna hay. Dry matter intake of sorghum hay mixed with lablab hay at 32 % was positively and strongly predicted from in sacco degradation after 12 (r2 = 0.90), 24 (r2 = 0.94), 48 (r2 = 0.96) and 72 (r2 = 0.97) hours of incubation. Dry matter intake of sorghum hay mixed with 39 % cowpea hay was poorly predicted from in sacco degradation after 12 (r2 = 0.25), 24 (r2 = 0.20), 48 (r2 = 0.11) and 72 (r2 = 0.08) hours of incubation. Dry matter intake of sorghum hay mixed with 23 % mucuna hay was positively and moderately predicted from in sacco degradation after 12 (r2 = 0.68) hours and 24 (r2 = 0.55) hours, but poorly predicted after 48 (r2 = 0.40) and 72 (r2 = 0.20) hours of incubation. vii It was concluded that cowpea, lablab and mucuna hays had higher protein contents and palatability indices than forage sorghum and therefore, have the potential of being utilised as protein supplements for goats on low quality roughages. Legume supplementation, in general, improved diet intake, digestibility, feed conversion ratio and live weight of goats, except for lablab hay supplementation. It was also noted that diet intake, digestibility and final live weights of the goats were optimised at different levels of forage supplementation. This may indicate that supplementation levels for optimum productivity will depend on the particular parameter in question. In vitro gas production and in sacco degradation techniques have the potential of predicting intake, digestibility and palatability indices of goats fed sorghum hay supplemented with cowpea, lablab or mucuna hays.