Typescript (photocopy). / Digitized by Kansas Correctional Industries

Long-term insect abundances have been studied to investigate factors structuring the insect communities of the temporary wetlands around the River Dalälven floodplain. In the wet meadows and swamps in the River Dalälven floodplains recurrent floods are the ultimate prerequisite for high production of floodwater mosquitoes. In the larval stages these make up a superabundant potential prey for aquatic predatory insects while as adults they become nuisance for people and animals. Mosquito control with Bacillus thuringiensis israelensis (Bti) has been carried out in parts of the floodplain wetlands since 2002. This study investigate potential long-term effects of Bti on non-target insects in general, and non-biting midges (Diptera: Chironomidae) and aquatic predator insects in special in 10 temporary wetlands. Diving-beetles (Coleoptera: Dytiscidae) were the most common aquatic predatory insects in temporary flooded wetlands, and we found no indications that diving-beetles were negatively affected by mosquito larvae reduction with Bti-treatments. Non-target Bti-effects on the insect community in general, measured as increase or decrease of insect abundances, in general showed no effects of Bti except for Coleoptera. The non-biting midges were studied on species level, since this taxon previously has been associated with negative Bti non-target effects in the short-term. We found no reduced production of chironomids at neither family, nor subfamily level in Bti-treated as compared to untreated wetlands. We conclude that other factors than the occasional Bti-treatments dominate in structuring the chironomids fauna. For example, hydrology measured as floods and hydroperiod had substantial impact on insect emergence Increased hydroperiod was associated with lower insect emergence for the majority of the studied taxa, indicating an insect fauna adapted more to terrestrial conditions. In the final study, we developed and applied a molecular method to study interspecific predator – prey relationships between medium-sized diving beetles and floodwater mosquito larvae. Gut content analyses showed that floodwater mosquito larvae are a regular, but limited, part of the diet of medium-sized diving beetles. This thesis is one of the first long-term studies of insects of temporary wetlands in relation to mosquito control actions. The results indicate that hydrology is one of the major factors influencing and structuring the insect communities of the temporary flooded wetlands in the River Dalälven floodplains, and that mosquito control actions with Bti only have marginal effect on insect abundances.

Impending natural resources development and concern about the effects of climate change have spurred increased efforts to study and monitor aquatic habitats in the Mackenzie River system. As part of Environment Canada’s attempt to survey the system in advance of the construction of the Mackenzie Gas Pipeline, benthic macroinvertebrates were sampled at 50 streams spanning the geographical range of the Mackenzie system in the Northwest Territories, Canada, to assess spatial patterns in diversity and assemblage structure and the environmental factors driving them. Replicated, quantitative D-net samples were collected during the late summer of 2005 through 2008, mostly at crossings of the proposed pipeline route. 373 macroinvertebrate taxa were recorded, mainly aquatic insects, which were identified to the genus or species levels; other groups were identified to higher taxonomic levels. Ephemeroptera and Plecoptera diversity declined along a latitudinal gradient, while Trichoptera diversity declined in the middle of the latitudinal range and rose towards the far north. Chironomidae (Diptera) increased in diversity and abundance towards the far north, becoming dominant in the northern sub-arctic forest and lowland tundra of the Mackenzie Delta. Diversity, measured as the average generic richness per stream, correlated with a composite environmental variable representing stream size, but not much else; spatial trends in local generic richness were only apparent in the far north of the study area. Regional diversity was assessed using rarefaction curves and showed a clear decrease from south to north across the study area for most taxa; the major exception was the chironomid subfamilies Orthocladiinae and Chironomini, the former being diverse throughout the study area and the latter increasing in diversity on the tundra. Odonata, Hemiptera and Coleoptera were well-represented in the south of the study area, but decreased sharply in diversity and abundance in the north; another common order, Megaloptera, was entirely absent from the study area, as were crayfish. Community composition varied along a latitudinal gradient, with some species restricted to northern latitudes and many more species restricted to the southern areas. Composition varied by region, as did the environmental factors that control it. Streams in the north of the system are connected to hundreds of small lakes and tend to freeze in the winter, which increases habitat stability; assemblages in this region were characterized by relatively large chironomids that are usually associated with lentic habitats and by a lack of taxa that are intolerant to freezing. Substrate was the main factor explaining differences in assemblage composition in this region. Just to the south, alluvial streams are more common and permafrost is continuous with very shallow active layers, iv which likely results in intense discharge peaks and ice scour in the spring and flashy summer hydrographs. Invertebrates in this region were mainly short-lived, small sized orthoclads, baetids and chloroperlids; the annual disturbance regime seems likely to be an important factor shaping community composition in this region. Many streams in this region received input from saline springs, resulting in perennial flow, and these streams harboured several taxa that were absent or rare in other streams at similar latitudes, including several stoneflies (e.g. Pteronarcys, Sweltsa); the presence of flow during the winter was found to be a major factor affecting community composition in this region, which surrounded the town of Norman Wells, NT. Nutrient dynamics appeared to be important in structuring benthic assemblages in the southern portion of the study region, with highnutrient streams supporting a diverse fauna which included many taxa that were absent in the north, while communities in low-nutrient streams were more similar to the northern alluvial stream fauna. There was no spatial distinction between low- and high-nutrient streams in the southern region, and the difference may be due to the local conditions of permafrost, which is patchy and discontinuous in the region. Evidence that winter ice and permafrost conditions are important drivers of benthic invertebrate diversity and community composition in the Mackenzie system, along with the latitudinal gradients which are consistent with a temperature/climate gradient, raises the possibility that benthic assemblages may be useful as indicators of effects of global climate change on freshwater habitats in the Canadian north. More immediately, construction of the Mackenzie Gas Pipeline may affect stream habitat due to sedimentation, and plans for the operation of the pipeline have raised concerns about potential effects on permafrost conditions. Implications for development of a biomonitoring program utilizing benthic invertebrates and their potential as indicators of climate change are discussed.

Impending natural resources development and concern about the effects of climate change have spurred increased efforts to study and monitor aquatic habitats in the Mackenzie River system. As part of Environment Canada’s attempt to survey the system in advance of the construction of the Mackenzie Gas Pipeline, benthic macroinvertebrates were sampled at 50 streams spanning the geographical range of the Mackenzie system in the Northwest Territories, Canada, to assess spatial patterns in diversity and assemblage structure and the environmental factors driving them. Replicated, quantitative D-net samples were collected during the late summer of 2005 through 2008, mostly at crossings of the proposed pipeline route. 373 macroinvertebrate taxa were recorded, mainly aquatic insects, which were identified to the genus or species levels; other groups were identified to higher taxonomic levels. Ephemeroptera and Plecoptera diversity declined along a latitudinal gradient, while Trichoptera diversity declined in the middle of the latitudinal range and rose towards the far north. Chironomidae (Diptera) increased in diversity and abundance towards the far north, becoming dominant in the northern sub-arctic forest and lowland tundra of the Mackenzie Delta. Diversity, measured as the average generic richness per stream, correlated with a composite environmental variable representing stream size, but not much else; spatial trends in local generic richness were only apparent in the far north of the study area. Regional diversity was assessed using rarefaction curves and showed a clear decrease from south to north across the study area for most taxa; the major exception was the chironomid subfamilies Orthocladiinae and Chironomini, the former being diverse throughout the study area and the latter increasing in diversity on the tundra. Odonata, Hemiptera and Coleoptera were well-represented in the south of the study area, but decreased sharply in diversity and abundance in the north; another common order, Megaloptera, was entirely absent from the study area, as were crayfish. Community composition varied along a latitudinal gradient, with some species restricted to northern latitudes and many more species restricted to the southern areas. Composition varied by region, as did the environmental factors that control it. Streams in the north of the system are connected to hundreds of small lakes and tend to freeze in the winter, which increases habitat stability; assemblages in this region were characterized by relatively large chironomids that are usually associated with lentic habitats and by a lack of taxa that are intolerant to freezing. Substrate was the main factor explaining differences in assemblage composition in this region. Just to the south, alluvial streams are more common and permafrost is continuous with very shallow active layers, iv which likely results in intense discharge peaks and ice scour in the spring and flashy summer hydrographs. Invertebrates in this region were mainly short-lived, small sized orthoclads, baetids and chloroperlids; the annual disturbance regime seems likely to be an important factor shaping community composition in this region. Many streams in this region received input from saline springs, resulting in perennial flow, and these streams harboured several taxa that were absent or rare in other streams at similar latitudes, including several stoneflies (e.g. Pteronarcys, Sweltsa); the presence of flow during the winter was found to be a major factor affecting community composition in this region, which surrounded the town of Norman Wells, NT. Nutrient dynamics appeared to be important in structuring benthic assemblages in the southern portion of the study region, with highnutrient streams supporting a diverse fauna which included many taxa that were absent in the north, while communities in low-nutrient streams were more similar to the northern alluvial stream fauna. There was no spatial distinction between low- and high-nutrient streams in the southern region, and the difference may be due to the local conditions of permafrost, which is patchy and discontinuous in the region. Evidence that winter ice and permafrost conditions are important drivers of benthic invertebrate diversity and community composition in the Mackenzie system, along with the latitudinal gradients which are consistent with a temperature/climate gradient, raises the possibility that benthic assemblages may be useful as indicators of effects of global climate change on freshwater habitats in the Canadian north. More immediately, construction of the Mackenzie Gas Pipeline may affect stream habitat due to sedimentation, and plans for the operation of the pipeline have raised concerns about potential effects on permafrost conditions. Implications for development of a biomonitoring program utilizing benthic invertebrates and their potential as indicators of climate change are discussed.

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Through wastewater entering aquatic environments, aquatic insects are continuously exposed to pharmaceuticals including neurologically active antihistamines. The antihistamine hydroxyzine has previously been found to lower activity in damselflies and to reach 2000 times the concentration of surrounding water in damselfly tissue. The purpose of this short-term exposure study was to investigate if hydroxyzine also bioaccumulates in dragonflies and if dilute hydroxyzine (362 ± 50, mean ng/l ± SD) have effects on predator-prey interactions between dragonfly Aeshna grandis and damselfly Coenagrion hastulatum larvae, i.e. number of attacks and predation success. Predators and prey were captured and exposed during one, three or five days (with controls) before taking part in predation experiments; Dragonflies were put in separate containers with six damselflies, they were video recorded and attacks and predated damselflies noted during four hours. Tissue concentrations of hydroxyzine were analyzed from all dragonflies and a subsample of the damselflies showing a mean bioconcentration factor (BCF) of 27 and 7 respectively, surprisingly much lower than previous research. There was no difference in attack rate or predation efficiency between controls and exposed dragonflies. However, dragonflies exposed for five days were found to attack more and capture more prey than dragonflies exposed for one day, a change that was not seen in the controls. This confounding factor motivates further studies to clarify if hydroxyzine after a period of exposure can have a sublethal effect altering foraging and/or predator avoidance traits with the net result of increased predation success for dragonflies in the predator-prey interaction between dragonflies and damselflies.

Thesis (Ph.D.)--Murdoch University, 2003. / Appendix 1 attached as CD-ROM. Thesis submitted to the Division of Science and Engineering. Bibliography: leaves [156]-173.