This research has examined the phylogenetic relationship between the questionable Athyrium pycnocarpon and the valid Athyrium thelypteroides. Classical taxonomic data were reported to illustrate the sources of confusion in the phylogenetic positioning of the fern in question. Chromatographic analyses of biochemical secondary constituents were reported. Descending paper chromatograms, ascending thinlayer chromatograms and cellulose-acetate electrophoretograms were the three analytical tools used to develop the secondary compounds. Ultraviolet light and ninhydrin visualization of the separated secondary constituents produced characteristic spotting patterns. On the basis of the close similarities of all patterns between the Athyrium pycnocarpon and Athyrium thelypteroides, it was concluded that the Athyrium pycnocarpon is unquestionably a member of the Athyrium complex.Ball State UniversityMuncie, IN 47306

This thesis has examined a long existing conflict between two ferns, Asplenium platyneuron and Athyrium pycnocarpon. The study included habitat, external and internal morphological characteristics, and biochemical analyses of the free amino acids. Major emphasis was placed upon vascular structure and arrangement in the stipes, roots, and rhizomes.Biochemically, the free amino acids were separated by one-dimensional descending paper chromatography. All areas investigated were either photographed or drawn and presented in the text of the thesis.Ball State UniversityMuncie, IN 47306