Background: Periodontitis is a chronic immuno-inflammatory disease characterized by inflammatory destruction of tooth-supporting tissues. Its pathogenesis involves a dysregulated local host immune response that is ineffective in combating microbial challenges. An integrated investigation of genes involved in mediating immune response suppression in periodontitis, based on multiple studies, can reveal genes pivotal to periodontitis pathogenesis. Here, we aimed to apply a deep learning (DL)-based autoencoder (AE) for predicting immunosuppression genes involved in periodontitis by integrating multiples omics datasets. Methods: Two periodontitis-related GEO transcriptomic datasets (GSE16134 and GSE10334) and immunosuppression genes identified from DisGeNET and HisgAtlas were included. Immunosuppression genes related to periodontitis in GSE16134 were used as input to build an AE, to identify the top disease-representative immunosuppression gene features. Using K-means clustering and ANOVA, immune subtype labels were assigned to disease samples and a support vector machine (SVM) classifier was constructed. This classifier was applied to a validation set (Immunosuppression genes related to periodontitis in GSE10334) for predicting sample labels, evaluating the accuracy of the AE. In addition, differentially expressed genes (DEGs), signaling pathways, and transcription factors (TFs) involved in immunosuppression and periodontitis were determined with an array of bioinformatics analysis. Shared DEGs common to DEGs differentiating periodontitis from controls and those differentiating the immune subtypes were considered as the key immunosuppression genes in periodontitis. Results: We produced representative molecular features and identified two immune subtypes in periodontitis using an AE. Two subtypes were also predicted in the validation set with the SVM classifier. Three “master” immunosuppression genes, PECAM1, FCGR3A, and FOS were identified as candidates pivotal to immunosuppressive mechanisms in periodontitis. Six transcription factors, NFKB1, FOS, JUN, HIF1A, STAT5B, and STAT4, were identified as central to the TFs-DEGs interaction network. The two immune subtypes were distinct in terms of their regulating pathways. Conclusion: This study applied a DL-based AE for the first time to identify immune subtypes of periodontitis and pivotal immunosuppression genes that discriminated periodontitis from the healthy. Key signaling pathways and TF-target DEGs that putatively mediate immune suppression in periodontitis were identified. PECAM1, FCGR3A, and FOS emerged as high-value biomarkers and candidate therapeutic targets for periodontitis.

Fraud is a common crime within the insurance industry, and insurance companies want to quickly identify fraudulent claimants as they often result in higher premiums for honest customers. Due to the digital transformation where the sheer volume and complexity of available data has grown, manual fraud detection is no longer suitable. This work aims to automate the detection of fraudulent claimants and gain practical insights into fraudulent behavior using unsupervised anomaly detection, which, compared to supervised methods, allows for a more cost-efficient and practical application in the insurance industry. To obtain interpretable results and benefit from the temporal dependencies in human behavior, we propose two variations of LSTM based autoencoders to classify sequences of insurance claims. Autoencoders can provide feature importances that give insight into the models' predictions, which is essential when models are put to practice. This approach relies on the assumption that outliers in the data are fraudulent. The models were trained and evaluated on a dataset we engineered using data from a Swedish insurance company, where the few labeled frauds that existed were solely used for validation and testing. Experimental results show state-of-the-art performance, and further evaluation shows that the combination of autoencoders and LSTMs are efficient but have similar performance to the employed baselines. This thesis provides an entry point for interested practitioners to learn key aspects of anomaly detection within fraud detection by thoroughly discussing the subject at hand and the details of our work. / <p>Gjordes digitalt via Zoom. </p>