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This master thesis deals with nanotechnology, nanoparticles and nanostructured surfaces, electrochemical methods, especially voltammetry, cyclic voltammetry, electrochemical impedance spectroscopy and contact angle measurement. One part is focused on electrodes primarily nanostructured and modified by substancies with thiol bound. Tutorial for preparation of gold nanostructured electrods is introduced in practical section including SEM photos of electrode surface. Nanostructured and bare gold electrodes were modified by 11-mercaptoundecanoic acid, streptavidin, glycine and biotin and measured by cyclic voltammetry, electrochemical impedance spectroscopy and contact angle.