Work-related musculoskeletal disorders (WMSDs) due to overexertion and consequently the low back pain (LBP) are one of the most prevalent sources of nonfatal occupational injuries and illnesses in all over the world. In the past several years, the industrial exoskeletons especially the passive ones have been proposed as alternative intervention and assistive devices, which are capable of reducing the risk of WMSDs and LBP. However, more research is warranted to validate the applicability of these exoskeletons. In addition, because the majority of previous studies have been limited to specific lifting tasks using only one type of lift assistive exoskeleton, more research is needed to examine the effect of alteration of different lift-assistive exoskeletons on reducing the activity of back muscles and metabolic reduction. The main objective of this dissertation is to render an overview of three studies that attempt to improve the literature by providing comprehensive biomechanical evaluations and metabolic assessments of three passive lift-assistive exoskeletons (VT-Lowe's Exoskeleton (developed in ARLab at VT), Laevo and SuitX). This dissertation has been composed of three related studies. The first study aimed to investigate and examine the capability of a novel lift assistive exoskeleton, VT-Lowe's exoskeleton, in reducing the peak and mean activity of back and leg muscles. Findings revealed that the exoskeleton significantly decreased the peak and mean activity of back muscles (IL(iliocostalis lumborum) and LT(longissimus thoracis)) by 31.5% and 29.3% respectively for symmetric lifts, and by 28.2% and 29.5% respectively for asymmetric lifts. Furthermore, the peak and mean EMG of leg muscles were significantly reduced by 19.1% and 14.1% during symmetric lifts, and 17.4% and 14.6% during asymmetric lifts. Interestingly, the VT-Lowe's exoskeleton showed higher reduction in activity of back and leg muscles compared to other passive lift-assistive exoskeletons available in the literatures. In the second study, the metabolic cost reduction associated with the use of VT-Lowe's exoskeleton during freestyle lifting was theoretically modelled, validated and corresponding metabolic savings were reported. The metabolic cost and the oxygen consumption results supported the hypothesis that the VT-Lowe's exoskeleton could significantly reduce the metabolic demands (~7.9% on average) and oxygen uptake (~8.7% on average) during freestyle lifting. Additionally, we presented a prediction model for the metabolic cost of exoskeleton during repetitive freestyle lifting tasks. The prediction models were very accurate as the absolute prediction errors were small for both 0% (< 1.4%) and 20% (< 0.7%) of body weight. In the third study, the biomechanical evaluation, energy expenditure and subjective assessments of two passive back-support exoskeletons (Laevo and SuitX) were examined in the context of repetitive lifting tasks. The experimental lifting tasks in this study were simulated in a laboratory environment for two different levels of lifting symmetry (symmetric vs. asymmetric) and lifting posture (standing vs. kneeling). Results of this study demonstrated that using both exoskeletons during dynamic lifting tasks could significantly lower the peak activity of trunk extensor muscles by ~10-28%. In addition, using both exoskeletons could save the energy expenditure by ~4-13% in all conditions tested by partially offsetting the weight of the torso. Such reductions were, though, task-dependent and differed between the two tested exoskeletons. Overall, the results of all three studies in this dissertation showed the capability of passive lift-assistive exoskeletons in reducing the activity of back and leg muscles and providing metabolic savings during repetitive lifting tasks. / Doctor of Philosophy / Low back pain (LBP) due to overexertion is known as one of the most important sources of nonfatal occupational injuries especially for the workers or manual material handlers who are involved in frequent or repetitive lifting tasks. Every year, many workers are temporarily or permanently disabled due to overuse injuries at workplace. In the past several years, industrial exoskeletons have gained growing interest among biomechanist, roboticist, and other human factor researchers as potential assistive devices to reduce the risk of LBP. In general, the industrial exoskeletons are either “passive or “active”; Active exoskeletons are powered by mechanical/electrical motors and actuators, however, the passive exoskeletons often work using cheaper devices such as gas or metal springs, elastic elements, etc. The exoskeletons discussed in this dissertation are categorized as passive rigid lower-back exoskeletons and they function by storing energy in a spring when the wearer bends and returning the stored energy when the wearer lifts. This dissertation consists of three studies that attempt to provide comprehensive biomechanical evaluations and metabolic assessments of three passive lift-assistive exoskeletons (i.e., VT-Lowe’s Exoskeleton, Laevo and SuitX). The first study examined the efficacy of a novel lift-assistive exoskeleton, VT-Lowe’s exoskeleton, in reducing the peak and mean activity of back and leg muscles. The results of this study demonstrated that the exoskeleton reduced the peak and mean activity of back and leg muscles for symmetric and asymmetric lifting tasks. VT-Lowe’s exoskeleton also showed higher reduction in activity of back muscles compared to other passive lift-assistive exoskeletons available in the literature. In the second study, the metabolic cost reduction with VT-Lowe’s exoskeleton was theoretically modeled and the modeling outcomes were compared to metabolic costs measurements when the exoskeleton was worn. The experimental findings of this study supported the applicability of the exoskeleton by significantly reducing the metabolic cost and oxygen uptake during the freestyle repetitive lifting tasks. Moreover, the prediction metabolic cost model of the exoskeleton showed high accuracy as the absolute prediction errors were within 1.5%. In the third study, the biomechanical evaluation, energy expenditure and subjective assessments of two passive back-support exoskeletons (Laevo and SuitX) were examined in repetitive lifting tasks. The lifting tasks of this study were simulated in a laboratory environment for two different levels of lifting symmetry (symmetric vs. asymmetric) and lifting posture (standing vs. kneeling). Findings of this study showed that both exoskeleton significantly lowered the peak activity of back muscles during the dynamic lifting tasks. Moreover, using both exoskeletons provided metabolic cost savings in all of the studies conditions. Overall, results obtained from the three studies in this dissertation verified the capability of these passive lift- vi assistive exoskeleton in reducing the activity of back and leg muscles and providing the metabolic savings during repetitive lifting tasks.