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Micro particle image velocimetry (microPIV) is a non-intrusive tool for visualizing flow in micron-scale conduits. Using this investigative instrument, two experimental studies were performed to understand flow behaviors in microfluidic channels - a sudden expansion step flow and laminar velocity profile variation in diffusion driven mixing. First, flow in a backward facing step feature (1:5 expansion ratio) in a microchannel was taken as the subject of microPIV flow visualization. The onset and development of a recirculation flow was studied as a function of flow rate. This flow pattern was further used to investigate two major parameters affecting microPIV measurements; the depth-of-focus and recording time-intervals between images in a microPIV image pair. The onset of recirculation was initiated at flow rates that correspond to Reynolds numbers, Re > 95, which is well beyond the typical working range of microfluidic devices (Re=0.01-10). The recirculation flow has a 3D structure due to the dimensions of the microchannel and the effect of no slip condition on the walls. Ensemble cross-correlation was found not to be sensitive to variations of depth-of-focus and the output flow fields were similar as long as the overall optical focus remained within the upper and lower bounds of the microchannel. However, variations of time intervals between images in a microPIV pair, resulted in quantitatively and qualitatively different flow patterns for a given constant flow rate and depth-of-focus. In the second experiment, the effect of the laminar velocity profile and its variation on mixing phenomena at the reduced scale is studied. It is shown that the diffusive mass flux between two miscible streams, flowing in a laminar regime in a microchannel, is enhanced if the velocity at their diffusion interface is increased. Based on this idea, an in-plane passive micromixing concept is proposed and implemented in a working device (sigma micromixer). This mixer shows considerable mixing performance by periodically varying the flow velocity profile, such that the maximum of the profile coincides with the transversely progressing diffusion fronts repeatedly throughout the mixing channel. microPIV has been used to visualize the behavior of laminar flow inside the micromixer device and to confirm the periodic variation of the velocity profile through the mixing channel.

Flow separation and reattachment happens in many fluid mechanical situations occurring in engineering applications as well as in nature. The flow over a backward-facing step represents a geometrically simple flow situation exhibiting both flow separation and reattachment. Broadly speaking there are only two important parameters in the problem, the Reynolds number(Re) based on the step height(h),and a geometrical parameter, referred to as the Expansion ratio(ER), defined as the downstream channel height to the upstream channel height. In spite of the relative simplicity of this geometry, the flow downstream is quite complex. The main focus of the present work is to elucidate the unsteady three-dimensional coherent structures present in this flow at large Re, Re>36,000,based on the step height(h). For this, we use velocity field measurements from Particle Image Velocimetry (PIV)in conjunction with hotwire anemometry measurements. The time-averaged structure of this flow is first studied in detail, including the effect of Reynolds number(Re) and Expansion Ratio(ER), on it. These studies show that at sufficiently large Re (Re>20,000), the reattachment length becomes independent of Re. The detailed internal structure of the separation bubble is also found to be independent of Re, but for Revalues that are relatively larger(Re>36,000). At large Re, the main effect of ER ,is found to be on the reattachment length, which increases with ER and saturates for ER values greater than about 1.8. The detailed internal structure of the separation bubble has been mapped at high Re and is found to be nearly the same for all ER, when the streamwise length is normalized by the reattachment length. In order to elucidate the unsteady coherent vortical structures, PIV measurements are done in two orthogonal planes downstream of the backward-facing step. These measurements are done for ER= 1.50 at large Re(Re=36,000) and in a large aspect ratio facility(AR= span length/step height= 24); the latter being important to avoid any effects due to span-wise confinement. In the spanwise plane parallel to the lower wall(x-z plane),instantaneous velocity fields show counter rotating vortex pairs, which is a signature of the three-dimensional vortical structures in this plane. Using conditional averaging, this counter-rotating vortex pair signature is captured right from upstream of the step, to well after reattachment. Spatial correlations are used to get the length scale of these coherent vortical structures, which varies substantially from the attached boundary layer before separation to the region after reattachment. The variation of these structures in the cross-stream (vertical) direction at reattachment and beyond gives an idea about their three dimensional shape. The circulation of these counter-rotating pairs is measured from the conditionally aver-aged fields, and is found to increase with streamwise distance reaching normalized circulation values (Γ/Uoh) of about 0.5 around reattachment. Velocity spectra downstream of the step show peaks corresponding to both the shear layer frequency(Stsl)and a relatively lower frequency that corresponds to large-scale shedding from the separation bubble (Stb); the latter in particular being quasi-periodic. Small amplitude sinusoidal forcing at the shedding frequency(Stb) is applied close to the step, by blowing and suction, to make the quasi-periodic shedding more regular. Measurements show that this has a very small effect on both the mean separation bubble and on the counter-rotating structures in the x-z plane. This mild forcing however enables phase locked PIV measurements to be made which shows the bubble shedding phenomenon in the cross-stream plane(side view or x-y plane). The phase-averaged velocity fields show significant variations from phase to phase. Although there is some hint of structures being shed, from these phase-averaged fields, it is not very clear. One of the primary reasons is the fact that the flow is effectively spanwise averaged, as the three-dimensional structures are not locked in the spanwise direction. To get a three dimensional view of the sheddin gphenomenon, it is necessary to lock the spanwise location with respect to the three-dimensional vortical structures before averaging across the different phases. We use the condition, u’<- urms, to locate the central plane between the counter-rotating structures, which in effect are the “legs” of the three-dimensional structure. With this condition, we effectively get a slice of the shedding cycle cutting through the “head” of the three-dimensional structure. Apart from this cut, we also get a cut between adjacent structures from the weak sweep events, with the condition u’<- urms. Using these conditions, on the phase-locked velocity fields, we effectively lock the structures in time, as well as in the spanwise direction. With this ,a clearer picture of the shedding process emerges. The flow is highly three-dimensional near reattachment and the shedding of the separation bubble is modulated in the spanwise direction owing to the three-dimensional hairpin like vortical structures in the flow. The separation bubble is seen bulged out and lifted high at locations where the head of the hairpin vortex passes, owing to the strong ejection of fluid caused by the vortical structure. On the other hand, outside the hairpin vortices, weak sweep events push the flow towards the wall and make it shallow and less prominent, with the shedding being very weak in this plane. From these observations, a three-dimensional picture of the flow is proposed.