From Summary: A survey of collagenolytic aerobic bacteria from cured hides yielded three strains of Bacillus and eight of Achromobacter which degraded collagen at 0.4 M NaCl. Achromobacter sp. 2 was chosen for genetic studies due to its high collagenolytic activity and the lack of genetic information on Achromobacter. Four temperate bacteriophages specific for Achromobacter sp. 2 were isolated and their relationships studied. The phages caused lysogenic conversion resulting in the inability of lysogens to adsorb phage. Achromobacter sp. 2 was shown to be a cryptic lysogen as it was not immune to superinfection but had a very low rate of spontaneous induction which could be increased with mutagens. It is proposed that the cryptic lysogeny of this strain is maintained by a defective excision mechanism and the mode of prophage integration in the host chromosome. DNA extracted from phage α3a was used to transfect spheroplasts. The optimal conditions for the development of competence for transfection were determined. The presence of nuclease-attack on phage DNA under conditions of prolonged incubation of DNA and spheroplasts was proposed. A method for extracting Achromobacter DNA was devised which yielded purified, undegraded DNA, but it was not possible to transform Achromobacter sp. 2 with this DNA. The a phages were used to transduce a number of genetic markers into Achromobacter auxotrophs. The transduct ants had the ability to release the cryptic α3 prophage at a high rate while maintaining their sensitivity to homologous phage infection. It is proposed that this is due to complementation between the cryptic prophage and the residual phage functions in the transducing particles. The transductants segregated auxotrophs with a probability of 10⁻³ per cell per generation. It appears that an unusual system of generalised transduction is operating whereby the transducing particles contain both phage and bacterial DNA which is incorporated into the recipient genome by a single recombination event yielding unstable transductants. In a study on induction of Escherichia coli (λ), carcinogenic nitrosamines were shown to be inducers of phage development. This provides a screening system for potentially harmful nitrosamines.

Bibliography: pages 147-177. / Nocardioforms are Gram-positive, aerobic actinomycetes and are a metabolically diverse group which produce antibiotics, useful enzymes, are important in the biotransformation of organic compounds and the decomposition of organic wastes and are important medically. A gene cloning vector designated pLR591 was constructed from the broad host range, multicopy Streptomyces plasmid pIJ702 and the positive selection Escherichia coli plasmid pEcoR251. This plasmid has useful features for the construction of actinomycete genomic libraries. Cloning of DNA into the unique Bg1II endonuclease site of pLR591 inactivated the lethal EcoRI gene derived from pEcoR251, thereby selecting for recombinant plasmids containing inserted DNA. The thiostrepton resistance gene derived from pIJ702 was shown to be functional in Streptomyces lividans enabling selection of recombinant pLR591 plasmids containing foreign DNA in S. lividans. The vector pLR591 therefore functions as a positive selection Streptomyces-E. coli shuttle vector facilitating construction of actinomycete genomic libraries in E. coli and subsequent transfer of recombinant plasmids into S. lividans.

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by Hon-chiu Leung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Bibliography: leaves 122-126. / Abstract --- p.i / Acknowledgement --- p.iv / Dedication --- p.v / Table of Contents --- p.vi / Introduction --- p.1 / Literature Review --- p.4 / Chapter 1) --- Ethanol production in bacteria / Chapter 1.1) --- Zymomonas mobilis --- p.4 / Chapter 1.2) --- Clostridium species --- p.7 / Chapter 1.3) --- "Enterobacter, Klebsiella, Serritia and Erwinia sp" --- p.9 / Chapter 1.4) --- Escherichia coli and Salmonella typhimurium --- p.10 / Chapter 2) --- Pyruvate decarboxylase of Z. mobilis / Chapter 2.1) --- Enzyme properties --- p.13 / Chapter 2.2) --- Cloning and expression of pdc gene --- p.15 / Chapter 3) --- Alcohol dehydrogenase (adh) gene / Chapter 3.1) --- "Cloning, chararterization and expression of adh genes" --- p.17 / Chapter 4) --- Gene transfer systems in Vibrio species --- p.21 / Chapter 5) --- Rationale and objectives of this study --- p.22 / Chapter Part I) --- Ethanol Production in terrestrial enteric bacteria / Chapter A) --- Introduction --- p.24 / Chapter B) --- Materials and Methods / Chapter 1) --- Bacterial strains and plasmids --- p.25 / Chapter 2) --- Media --- p.26 / Chapter 3) --- Solutions --- p.27 / Chapter 4) --- Isolation of plasmids / Chapter 4.1) --- Small Scale Isolation of plasmids --- p.30 / Chapter 4.2) --- Large Scale Isolation of plasmids --- p.32 / Chapter 5) --- Construction of a broad-host-range plasmid harbouring Zymomonas mobilis genes --- p.35 / Chapter 6) --- Transformation --- p.35 / Chapter 7) --- High Performance Liquid Chromatography of Organic Acids --- p.36 / Chapter 8) --- Maintenace of plasmids harbouring the genes of Zymomonas mobilis genes --- p.38 / Chapter 9) --- Ethanol tolerance of S. typhimurium strains --- p.38 / Chapter C) --- Results / Chapter 1) --- Construction of Salmonella typhimurium strains harbouring Z. mobilis genes --- p.39 / Chapter 2) --- Fermentative end products in culture medium --- p.48 / Chapter 3) --- Growth of hosts and transformants --- p.61 / Chapter 4) --- Ethanol tolerance of S. typhimurium strains --- p.65 / Chapter 5) --- Maintenance of plasmids --- p.67 / Chapter 6) --- Construction of broad-host-range plasmid harbouring Z. mobilis genes --- p.69 / Chapter D) --- Discussions / Chapter 1) --- Comparison of ethanol production in Escherichia coli and Salmonella typhimurium --- p.72 / Chapter 2) --- Ethanol tolerance of S. typhimurium strains --- p.74 / Chapter 3) --- Maintenance of plasmids --- p.76 / Chapter 4) --- Construction of broad-host-range plasmids harbouring Z. mobilis genes --- p.78 / Chapter Part II) --- Ethanol Production in marine enteric bacteria / Chapter A) --- Introduction --- p.79 / Chapter B) --- Materials and Methods / Chapter 1) --- Bacterial strains and plasmids --- p.80 / Chapter 2) --- Media --- p.80 / Chapter 3) --- Solutions --- p.80 / Chapter 4) --- Routine Identification Processes --- p.81 / Chapter 5) --- Systematic studies by Arbitrarily- Primed Polymerase Chain Reaction --- p.86 / Chapter 6) --- Optimal growth conditions --- p.88 / Chapter 7) --- Isolation of broad-host-range plasmid pIOl ( 64kb) --- p.89 / Chapter 8) --- Transformation of Vibrio sp. strain 60 --- p.90 / Chapter 9) --- Production of ethanol using different carbon sources in fermentation --- p.91 / Chapter C) --- Results / Chapter 1) --- Identification of Vibrio sp. strain 60 --- p.92 / Chapter 2) --- Optimal growth conditions --- p.101 / Chapter 3) --- Isolation of high molecular weight plasmid --- p.105 / Chapter 4) --- Ethanol production from different carbon sources --- p.107 / Chapter 5) --- Ethanol tolerance of Vibrio sp. strain 60 --- p.109 / Chapter 6) --- Salt tolerance of Vibrio sp. strain 60 --- p.111 / Chapter 7) --- Transformation of Vibrio sp. strain 60 --- p.113 / Chapter D) --- Discussions / Chapter 1) --- Strain identification by arbitrarily-primed PCR --- p.116 / Chapter 2) --- Isolation of high molecular weight plasmid --- p.118 / Chapter 3) --- Ethanol production of Vibrio sp. strain60 --- p.120 / References --- p.122

"March, 2005" / Bibliography: leaves 144-155. / ix, 155 p. : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / "The aims of this thesis were to investigate potentially novel aspects of the regulation of B and morphogenetic gene expression in coliphage 186, in order to understand more fully how late gene expression is controlled in this phage, and how gene expression may be regulated in general. Three specific aims were pursued in this project: 1. to characterize E. coli mutants that appear to abolish 186 B protein activity; 2. to determine the role of replication for the provision of late functions during the phage lytic cycle; and 3. to determine the role of CI repression of the 186 B promoter." --p. 41. / Thesis (Ph.D.)--University of Adelaide, School of Molecular and Biomedical Sciences, Discipline of Biochemistry, 2005

Thesis (Ph.D.)--Kent State University, 2009. / Title from PDF t.p. (viewed Jan. 12, 2010). Advisor: Laura G. Leff. Keywords: Bacteria; modeled reduced gravity; response; gene expression; physiology. Includes bibliographical references (p. 200-204).

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences