Miu Fei Fei. / On t.p. "42" are subscripts following the word "MSP-1" in the title. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 117-125). / Abstracts in English and Chinese. / ACKNOWLEGEMENTS --- p.i / ABSTRACT --- p.ii / TABLE OF CONTENTS --- p.v / LIST OF FIGURE --- p.vii / LIST OF ABBREVIATIONS --- p.ix / CHAPTERS: / Chapter 1. --- BACKGROUND OF MALARIA / Chapter 1.1 --- Epidemilogy --- p.2 / Chapter 1.2 --- Mode of Infection --- p.4 / Chapter 1.3 --- Conventional Control & Vaccination --- p.9 / Chapter 1.4 --- Vaccine Candidate PfMSV-142 --- p.16 / Chapter 1.5 --- Cloning and Expression of pfMSP-142 --- p.26 / Chapter 1.6 --- Aims of Study --- p.32 / Chapter 2. --- Materials and Methods / Chapter 2.1 --- Materials --- p.32 / Chapter 2.2 --- Methods --- p.38 / Chapter 3. --- Construction of recombinants N-PfMSP-142 and C- PfMSP-142 / Chapter 3.1 --- Construction of C-PfMSP-l42 --- p.51 / Chapter 3.2 --- Construction ofN-PfMSP-l42 --- p.56 / Chapter 4. --- Purification with IMAC / Chapter 4.1 --- Immobilized Metal Affinity Chromatography (IMAC) --- p.58 / Chapter 4.2 --- Purification ofN-PfMSP-l42 --- p.61 / Chapter 4.3 --- Purification profile of N-PfMSP-142 --- p.68 / Chapter 4.4 --- Purification of C-PfMSP-l42 --- p.70 / Chapter 4.5 --- Purification profile of C-PfMSP-142 --- p.73 / Chapter 4.6 --- Expression pattern of recombinants PfMSP-142 --- p.76 / Chapter 5. --- Purification combined with other chromatography method / Chapter 5.1 --- Affinity chromatography --- p.78 / Chapter 5.2 --- Gel filtration chromatography --- p.80 / Chapter 5.3 --- Ion exchange chromatography --- p.83 / Chapter 5.4 --- Conclusion --- p.93 / Chapter 6. --- Characteristic of IMAC products --- p.94 / Chapter 7. --- Characteristic of N-hisPfMSP-l42 & C-hisPfMSP-l --- p.42 / Chapter 7.1 --- Immunogenitcity of N-PfMSP-l42 and C-PfMSP-142 --- p.100 / Chapter 7.2 --- Competitive ELISA --- p.105 / Chapter 8. --- Discussion --- p.107 / REFERENCE --- p.117