The hydrolysis of adenosine, 2-deoxyadenosine and 6-chloropurine riboside by adenosine deaminase preparations from human spleen has been investigated, and Km and Vm values have been determined. The effect of the substrate on the reaction velocity was followed over a 250-fold range Results showed no deviation from Michaelis-Menten kinetics. The effect of pH on Vm, Vm/Km, and the apparent activation energy was examined. Inactivation of the enzyme by p-chloromercuribenzoate suggests the involvement of one or more SH groups. Competitive inhibition by inosine and the fact that ammonia is not, an inhibitor support a reaction mechanism involving a ternary complex. The apparent activation energy of the a parameter was smaller or less sensitive to temperature than the ß parameter.Ball State UniversityMuncie, IN 47306

The investigation of this thesis is introduced by finding all the relationships existing among the various types of set functions. Characterizations of the different set functions will be formulated in order to facilitate the construction and identification of each type of set function considered. This problem has important applications to measure theory and integration.Ball State UniversityMuncie, IN 47306

In this thesis, it is contemplated to study the asymptotic behaviour of the solutions of the differential equationz2d2y + z(bo +b1zm ) dy +(co + c1zm + c2 z2m)y =0 (1) dz2 dzHere m is a positive integer, the variable z is regarded complex as are the constants bi(i=0,1) and ci(i=0,1,2) with c2 ≠ 0. Then in the language of Fuch's theory the differential equation (1) will have a regular singular point at z=0 and an irregular singular point at z=θ. The indicial equation about z=0 is found to beh(h-1) + boh + co = 0(2)It is also assumed that the difference of the roots of (2) are incongruent to zero modulo m.Ball State UniversityMuncie, IN 47306

This study was concerned with the origin and stratigraphic relationship of a ridge-swales complex located in and near Mount Pleasant, Indiana. Borehole data, water well logs, seismic refraction surveys, and electrical resistivity surveys were combined to create three cross-sections which illustrate the stratigraphic relationship of the feature under scrutiny. In addition, textural analyses of outwash, esker, and ridge samples were performed to discriminate between outwash and esker material; the results were compared to the ridge samples.The textural analyses failed to discriminate convincingly between esker and outwash; however, the cross-sections display the ridge-valley complex as a broad sheet of outwash sand and gravel subsequently eroded by glacial meltwater.Ball State UniversityMuncie, IN 47306

A comparative study on the age changes in rat skeletal muscle tissue was performed using rats of different ages. The gastrocnemius muscle from rats of 3, 6, and 22 months of age was removed and examined for the presence of Type I fibers. Myosin ATPase histochemistry was used to differentiate between Type I and Type II fibers. A count of the number of Type I fibers in the entire gastrocnemius was made using fiber maps obtained from the stained muscle samples. The total number of fibers in some of the muscle samples was also determined.The results indicated a steady decline in the total number of muscle fibers from the ages of 3 to 22 months. With the exception of the 6 month group, the number of Type I fibers remained fairly constant. However, there was a definite increase in the percentage of Type I fibers found in the 22 month rats. An increase in the diameter of the muscle fibers in the 22 month rats was also observed.Ball State UniversityMuncie, IN 47306

The purpose of this investigation was to determine the relative importance of strength and anaerobic lactate metabolism to swimming performance. Although strength has been shown to play an integral part in swim performance, its development is not clearly understood.In addition, the emphasis to be placed on strength vs. anaerobic lactate utilization was examined in the various acre groups.Ball State UniversityMuncie, IN 47306

Factors influencing the distribution of Volvox spp. in 41 ponds of the Lake Itasca region, Minnesota, were investigated from May 25 through July 14, 1980. Twenty-two ponds had Volvox populations. The three species of Volvox found were: V. aureus Ehr., V. globator L. and V. tertius Meyer. Usually only one species was present in a pond with rare co-occurrence of two species. Only two of the 41 ponds contained two Volvox species simultaneously for a short time.Group comparison tests of ponds with and without Volvox showed three parameters to be significant (P > 0.05 level). Mean total phosphorus was higher in ponds with Volvox (x = 1.75 uM/1) than without (x = 0.95 uM/1). Mean temperature (20.4°C versus 21.8°C) and mean pH calculated from H+ concentrations (6.32 versus 6.76) were both lower in ponds with Volvox than in ponds without. Field observations of one pond revealed a patchy distribution of V. globator related to macrophyte abundance. Laboratory investigations confirmed that V. globator colonies orient to the aquatic moss Drepanocladus revolvens.Ball State UniversityMuncie, IN 47306

Ball State University LibrariesLibrary services and resources for knowledge buildingMasters ThesesThere is no abstract available for this thesis.

Microbial action on particulate chitin was analyzed in an eastcentral Indiana borrow pit lake. Experiments were conducted to determine the effects of water depth, particle size, mesh size of nylon bag containing chitin, and season on the decomposition process. The majority of chitinolytic bacteria isolated were classified as actinomycetes and pseudomonads. Results indicate that the sediment-water interface is the most active site of chitin mineralization in the lake environment. The samples seeded during summer showed the fastest rate of decomposition with greater than 50% weight loss after two weeks and greater than 95% weight loss after 7 weeks incubation in situ. The highest rate of decomposition (43.6 mg/g/day) occurred during this period. Samples seeded during the spring showed considerably slower activity with only a 25`o weight loss after 9 weeks incubation in situ. Fall samples showed a 30% weight loss after 9 weeks incubation in situ. The rate of degradation was highly correlated with water temperature. Significantly higher rates were-.noted with smaller particle size but no significant difference was evidenced with varying mesh size fran 10 to 100 in. The majority of chitinolytic bacteria isolated were Gram negative; Pseudanonas, Chranobacterium, Flavobacterium, and Serratia spp. Gram positive organisms showing chitin hydrolysis were predominantly actinomycetes. An active chitinolytic culture of Pseudcmonas sp. was used for enzyme studies. This culture was purified using (NH4)2SO4 precipitation, anion exchange chromatography and hydroxylapatite chromatography.Ball State UniversityMuncie, IN 47306

Ball State University LibrariesLibrary services and resources for knowledge buildingMasters ThesesThere is no abstract available for this thesis.