"February 2004" / Bibliography: leaves 150-185. / xiii, 185 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / "This project was designed to investgate new resistant populations of Hordeum spp; to assess the role of gene flow in spreading resistance; to determine the fitness of resistant individuals in accelerating resistance evolution; and to identify genetic relationships between resistant and susceptible populations using DNA markers." / Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Faculty of Sciences, 2005

Wild barley (Hordeum vulgare subsp. spontaneum) could be a source of useful genes for improving cultivated barley. The useful genes present in Hordeum vulgare subsp. spontaneum may be new alleles at described loci, or these may be entirely new genes in the sense that there is limited allelic variation at these loci in the cultivated germplasm pool. This research was directed at gene discovery in wild barley and involved two steps: (i) characterization of diversity using genetic markers and (ii) development and characterization of novel germplasm for gene discovery. Simple Sequence Repeats (SSRs) of known map location were used to survey three representative groups of barley germplasm: a sample of crop progenitor (Hordeum vulgare subsp. spontaneum) accessions, a group of mapping population parents, and a group of varieties and elite breeding lines. The objectives were to determine the informativeness and utility of SSRs in differentiating and classifying the three sets of barley germplasm. Crop progenitors had the highest number of alleles per SSR locus, followed by mapping population parents and elite breeding lines. The cluster analysis indicated a high level of diversity within the crop progenitor accessions and within the mapping population parents. It revealed a much lower level of diversity within the elite breeding germplasm. A set of Recombinant Chromosome Substitution Lines (RCSLs) representing introgressions of Hordeum vulgare subsp. spontaneum genome in to a cultivated barley background were developed using the Advanced Backcross strategy. An accession of Hordeum vulgare subsp. spontaneum was the donor parent and the variety "Harrington" was the recurrent parent. The RCSLs were developed via two backcrosses to the recurrent parent followed by six generations of selfing. The genomic architecture of the RCSLs was determined by molecular marker fingerprinting with SSRs. The consequences of introgressions of Hordeum vulgare subsp. spontaneum genome segments into the recurrent parent were assessed in terms of inflorescence yield components, malting quality traits, and domestication-related traits. Hordeum spontaneum subsp. spontaneum, despite its overall inferior phenotype, contributed favorable alleles for some characters of agronomic performance. In other cases, the introgressions caused a disruption of the Harrington phenotype, a "reverse genetics" approach to gene discovery. / Graduation date: 2003

No description available.

Four experiments were conducted with the objectives of (i) comparing the suitability of various H. bulbosum clones for haploid production, (ii) determining the parental effects of H. bulbosum and barley genotypes on percentage of pollinated florets yielding caryopses with rescuable embryos and on embryo viability, (iii) comparing different stages for embryo culture and caryopsis culture, and (iv) attempting to produce barley haploids directly from cultured immature caryopses. The results demonstrated: that reproductive characteristics of H. bulbosum clones varied with environmental conditions; that the hybrid H. bulbosum clones MBC-3 and MBC-4 were superior to their parents Cb2920 and Cb2929 as pollen donors; that both parental genotypes and date of harvesting after pollination had large effects on percentage of pollinated florets yielding caryopses with rescuable embryos and on embryo viability; that haploid plantlets can be generated from haploid caryopsis culture without embryo rescue, but only at a low frequency and with a slow rate of germination.

Bibliography: leaves 218-232. / 232 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis investigates the resistance response of barley towards R. secalis by examining the in vitro interaction between various components of the apoplastic fluid and the fungus. / Thesis (Ph.D.)--Adelaide University, Dept. of Applied and Molecular Ecology, 2001

Thesis (Ph. D.)--University of Adelaide, 1986. / Includes bibliographical references (leaves 173-200).

Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Science, 1996? / Includes bibliographical references (leaves 121-141).

Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Science, 1996. / Includes bibliographical references (leaves 223-247).

Thesis (M.Ag.Sc. )--University of Adelaide, Dept. of Plant Physiology, 1965. / Typecript. Includes bibliographical references.

Thesis (Ph.D.) -- University of Adelaide, Dept. of Plant Science, Waite Agricultural Research Institute, 1993.