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Virulence spectrum, molecular characterisation and fungicide sensitivity of the South African Rhynchosporium secalis populationRobbertse, Barbara 12 1900 (has links)
Thesis (PhDAgric)--University of Stellenbosch, 2000. / ENGLISH ABSTRACT: Barley leaf scald, caused by Rhynchosporium secalis, is the most important disease of
barley (Hordeum vulgare) in the Western Cape province of South Africa. The disease
was first reported from South Africa in 1937. The present study is the first attempt to
characterise the South African R. secalis population. Topics such as pathogenesisrelated
proteins, virulence spectra, variability of pathotypes, sources of variation, host
resistance, breeding strategies, molecular characterisation and fungicide sensitivity are
summarised in Part 1 of this dissertation. In succeeding Parts the focus is on the
characteristics of the local R. secalis population regarding virulence spectrum, DNA
polymorphisms, in vitro as well as in vivo fungicide sensitivity. These aspects are
treated as separate entities, leading to some duplication which is unavoidable.
In Part 2 the virulence spectra of 50 R. secalis isolates from a population in the.
Western Cape province were determined. Twenty-one races were detected using 17
differential barley cultivars. The two most prevalent races, namely races 4 and 7 had
three and four virulence genes respectively. Both race 4 and 7 were virulent on the
most susceptible cultivars, namely West China, Steudelli, C.I.8618 and C.I.2226.
Considering the resistance genes reported for cultivars Atlas 46, Turk, and C.I.3515
which showed no susceptible cultivar-pathogen interaction, it would appear that the Rh-
Rh3-Rh4 complex is primarily involved in conferring resistance to the local R. secalis
isolates.
A total of 20 races (47 isolates) characterised in Part 2 were selected for further
characterisation by means of DNA fingerprinting. In Part 3 an anonymous multilocus
DNA probe was used to characterise the genotypic structure of these isolates by means
of RFLP analysis. No correlation between any particular fingerprint pattern, race,
district, field or lesion was found. The two most prevalent races, 4 and 7, did not share
the same genotypes, even when isolated from the same field or lesion. The genotypic
diversity of the isolates studied was 46.5% of the theoretical maximum diversity. The
high level of genotypic variation observed in the South African R. secalis population
resembled the genotypic diversity observed in other cereal pathogens with known
sexual structures. Although no teleomorph has yet been observed, these data suggest
that sexual recombination may operate within the local population of R. secalis. In South Africa barley scald is primarily controlled by means of fungicides. The
continued use of fungicides on cereal crops results in the build-up of fungicide
resistance in the population, which could lower the efficacy of these compounds. These
aspects were investigated in Part 4, where isolates (collected during 1993 to 1995) were
evaluated in vitro for sensitivity to triadimenol, tebuconazole, flusilazole and
propiconazole. The sensitivity fluctuated but in 1995 isolates were significantly less
sensitive towards triadimenol than in the previous two years. In a second experiment,
isolates collected from two fields with a 5-6 year-history of triadimenol seed treatments
and tebuconazole applications, were evaluated for their fungicide sensitivity. A
significant positive correlation was observed between tebuconazole and triadimenol
sensitivity among,R. secalis populations from these fields. However, such a correlation
was not found within the R. secalis population collected during 1993-1995 where
shorter crop rotation patterns and a range of fungicides was applied. In a third
experiment, the fungicide sensitivity of local R. secalis isolates was evaluated towards
two new triazole fungicides, namely bromuconazole and triticonazole. Correlation
coefficients observed between these new triazoles and those previously applied in South
Africa were not significantly positive. The lack of significant cross-resistance has
important practical implications regarding the management of fungicide resistance.
In Part 5, isolates with different minimum inhibitory concentration (MIC)
towards tebuconazole in vitro (1, 3 and 10 ug/ml) were compared in vivo. The aim of
this study was to determine how MIC values would influence virulence (leaf area
affected) and sporulation. Results indicated that all isolates were equally fit to induce
lesions and sporulate in the absence of tebuconazole. Thus no fitness cost was
associated with the degree of tebuconazole sensitivity in the present study. All R.
secalis isolates were able to induce lesions on tebuconazole treated leaves, but differed
significantly with respect to the percentage leaf area affected. Isolates, least sensitive
(MIC = 10 ug/rnl) towards tebuconazole were more adapted on tebuconazole treated
leaves, being able to repeatedly cause larger lesions than sensitive R. secalis isolates
(MIC = 1 ug/rnl), Sporulation was not significantly different between isolates on
lesions of untreated or tebuconazole treated leaves. Larger leaf areas affected and
adequate sporulation suggest that a less sensitive population would result in more
disease in tebuconazole treated fields. In conclusion, this study revealed the variability associated with the South
African R. secalis population regarding virulence spectrum and genotypic structure.
The data in this study suggest that it is likely that the local population will easily adapt
to newly introduced, single gene resistance. For more durable resistance, higher levels
of quantitative resistance should be introduced. This type of resistance is, however,
more difficult to identify and incorporate than single gene resistance. Consequently,
barley scald control will remain dependent on the efficacy of fungicide applications.
Furthermore, the lack of cross-resistance and low frequency of resistant isolates
indicates a low risk for the development of fungicide resistance in the local R. secalis
population. Other factors such as current crop rotation practices and the range of
fungicides being ~pplied also contribute to this low risk level. However, the status of
these factors can change over time. The in vivo tebuconazole sensitivity study has
indicated that a resistant field population of R. secalis may be able to build-up. It is,
therefore, necessary to monitor the fungicide sensitivity of R. secalis isolates at timely
intervals with view to successful barley cultivation in the future. / AFRIKAANSE OPSOMMING: Blaarvlek op gars (Hordeum vulgare), veroorsaak deur Rhynchosporium secalis, is die
belangrikste siekte van gars in die Wes-Kaap provinsie van Suid-Afrika. Die voorkoms
van R. secalis op gars is in Suid-Afrika vir die eertse keer in 1937 gerapporteer. Hierdie
studie is die eerste poging tot karakterisering van die plaaslike R. secalis-populasie.
Aspekte soos proteïene betrokke by patogenese, virulensiespektra, variabiliteit van
patotipes, bronne van variasie, gasheerweerstand, teeltprogramme, molekulêre
karakterisering en swamdodersensitiwiteit word in Deel I van die tesis opgesom. In die
daaropvolgende gedeelte is die fokus op die karakterisering van die R. secalis-populasie
en behels DNA karakterisering, virulensiespektrum, en swamdodersensitiwiteit in vitro
asook in vivo. ..
In Deel 2 is die virulensiespektra van 50 R. secalis isolate van 'n populasie in die.
Wes-Kaap geëvalueer teenoor 17 differensiëel weerstandbiedende gars kultivars en
hieruit is 21 rasse geïdentifiseer. Die twee mees algemene rasse (rasse 4 en 7), met
onderskeidelik drie en vier virulensie gene, het virulent vertoon teenoor die mees
vatbare kultivars soos West China, Steudelli, C.I.8618 en C.I.2226. Geen vatbare
kultivar-patogeen interaksies is met kultivars Atlas 46, Turk en C.I.3515, wat al drie die
Rh-Rh3-Rh4 kompleks dra, gevind nie. Dit wil dus voorkom asof hierdie genekompleks
effektiewe gasheerweerstand teen die plaaslike R. secalis isolate kan bied.
'n Totaal van 20 rasse (47 isolate), gekarakteriseer in Deel 2, is geselekteer vir
verdere karakterisering met behulp van DNA bandpatrone. In Deel 3 is 'n anonieme
multilokus DNA peiler gebruik om deur middel van RFLP analise die genotipiese
struktuur van hierdie R. secalis-isolate te bepaal. Geen assosiasie is gevind tussen DNA
bandpatroon en ras, distrik, garsland of letsel nie. Die twee rasse (4 en 7) wat mees
algemeen voorkom, het nie dieselfde bandpatroon vertoon nie, ook nie dié afkomstig
vanuit dieselfde garsland of letsel nie. Die genotipiese diversiteit van isolate was 46.5%
van die teoretiese maksimum diversiteit. Die hoë vlak van variasie waargeneem in die
R. secalis populasie is soortgelyk aan variasie waargeneem in ander graanpatogene wat
oor 'n geslagtelike stadium in die lewenssiklus beskik. Alhoewel geen geslagtelike
stadium tot dusver geidentifiseer is nie, dui die vlak van variasie daarop dat geslagtelike
rekombinasie moontlik wel plaasvind binne die plaaslike R. secalis populasie. In Suid-Afrika word blaarvlek op gars primêr deur swamdoders beheer. Die
toenemende gebruik van swamdoders op graangewasse veroorsaak moontlik 'n opbou
van swamdoderweerstand in die populasie. Dit kan die effektiwiteit van swamdoders
verlaag. Hierdie veronderstelling is in Deel 4 ondersoek, waar die sensitiwiteit van
isolate in vitro teenoor triadimenol, tebukonasool, flusilasool en propikonasool
geëvalueer is. Die triasooi sensitiwiteit van R. secalis isolate wat gedurende die 1993-
1995 seisoen versamel is het gewissel en slegs vir triadimenol was daar 'n tendens na
meer weerstandbiedenheid. 'n Swamdoder-evaluasie is in 'n aparte eksperiment op
isolate gedoen wat versamel is vanaf twee garslande met 'n 5-6 jaar geskiedenis van
triadimenol saadbehandelings en tebukonasool bespuitings. 'n Betekenisvolle positiewe
korrelasie is waaJ~geneem tussen tebukonasool en triadimenol sensitiwiteit in R. secalis
isolate afkomstig vanaf hierdie twee garslande. 'n Soortgelyke korrelasie is egter nie
gevind in die populasie wat gedurende die 1993-1995 seisoene versamel IS me.
Laasgenoemde kan moontlik toegeskryf word aan korter wisselboupatrone en die
toediening van 'n verskeidenheid van swamdoders. In 'n derde eksperiment is die
sensitiwiteit van plaaslike R. secalis isolate teenoor twee nuwe triasole, naamlik
bromukonasool en tritikonasool getoets. Die korrelasie waargeneem tussen die twee
nuwe triasole en triasooi swamdoders reeds voorheen in gebruik in die Wes-Kaap was
me betekenisvol positief me. Die gebrek aan betekenisvolle kruisweerstandbiedendheid
het belangrike praktiese implikasies vir die bestuur van
swamdoder -weerstandbiedendheid.
In Deel 5 is isolate met wisselende minimum inhiberende konsentrasies (MIKs)
teenoor tebukonasool in vitro (1, 3 en 10 ug/ml) en in vivo vergelyk. Die doel van
hierdie studie was om te bepaal hoe wisselende MIK-waardes virulensie
(blaaroppervlakte geïnfekteer) en sporulasie sal beïnvloed. Resultate dui daarop dat alle
R. secalis isolate in hierdie studie ewe fiks was om, in die afwesigheid van
tebukonasool, letsels te induseer en te sporuleer. Die bevinding is dat die verlies in
fiksheid nie geassosieer is met die mate van tebukonasool weerstand nie. Alle R. secalis
isolate het die vermoë gehad om letsels op tebukonasool-behandelde blare te veroorsaak
maar het betekenisvol verskil ten opsigte van die blaaroppervlakte geaffekteer. Isolate
wat minder sensitief (MIK = 10 ug/rnl) teenoor tebukonasool in vitro is, het meer
aangepastheid op tebukonasool-behandelde blare getoon. Gevolglik het hierdie isolate
herhaaldelik meer letsels veroorsaak as sensitiewe isolate (MIK = 1 ug/ml), Sporulasie het nie betekenisvol verskil tussen isolate vanaf letsels op ondehandelde of tebukonsoolbehandelde
blare nie. Hierdie resultate dui egter daarop dat 'n minder sensitiewe
populasie tot meer siektevoorkoms in tebukonasool-bespuite lande kan lei.
Die studie het die veranderlike karakter van die Suid-Afrikaanse R. secalispopulasie
aangaande virulensiespektrum en genotipiese struktuur blootgelê. Dit is dus
baie moontlik dat die R. secalis-populasie maklik sal aanpas by teelmateriaal met nuwe
enkelgeen-weerstand. Vir volgehoue gasheerweerstand is dit egter nodig dat hoër
vlakke van kwantitatiewe weerstand ingeteel moet word. In die praktyk is hierdie tipe
weerstand egter baie moeiliker om te identifiseer en by nuwe teelmateriaal in te sluit as
in die geval van enkelgeen-weerstand, Dit bring mee dat blaarvlekbeheer afhanklik bly
van swamdodertoedienings as beheermaatreël. Die resultate van hierdie studie dui
daarop dat daar tans 'n lae risiko vir die ontwikkeling van swamdoderweerstand in die
plaaslike populasie is, as gevolg van die afwesigheid van kruisweerstandbiedendheid en
die lae voorkoms van weerstandbiediende isolate. Ander faktore soos die
wisselboustelsels wat toegepas word en die verskeidenheid van swamdoders toegedien
dra ook daartoe by. Ten spyte hiervan kan die status van hierdie faktore egter oor tyd
verander. Die in vivo tebukonasool studie het daarop gedui dat 'n weerstandbiedende
veldpopulasie van R. secalis die potensiaal het om te vermeerder. Gevolglik is die
tydige monitering van swamdodersenisitiwiteit van R. secalis isolate noodsaaklik om 'n
volhoubare garsproduksie te verseker.
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Species of Pythium associated with barley in South Australia / by J.I. BratoloveanuBratoloveanu, J. I. January 1985 (has links)
Bibliography: leaves 140-158 / ix, 158 leaves, [23] leaves of plates : ill., 1 map ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.1985) Dept. of Plant Pathology, University of Adelaide
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Ecology of Mediterranean snails in Southern Australian agriculture : a study of Cernuella virgata and Cochlicella acuta on the Yorke Peninsula / Vanessa L. Carne.Carne, Vanessa Lynne January 2003 (has links)
"August 2003." / Includes bibliographical references (leaves 294-333) / 2 v. (xxxi, 333 leaves) ; ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Discipline of Plant and Pest Science, 2005
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The genetic basis of barley black point formation.March, Timothy January 2008 (has links)
Black point of barley grain refers to a discolouration of the embryo end of the grain. Historically black point has been proposed to be due to fungal colonisation of the grain. However, Koch’s postulates have yet to be satisfied. The discolouration occurs during grain fill in response to high humidity or rainfall during the grain filling period. In wheat, which is also affected by black point, the discolouration has been proposed to be due to the oxidation of phenolic acids within the grain to form discoloured end products. Within this study, two approaches were investigated in order to understand the proteins and genes associated with this disorder. Firstly, a proteomics approach enabled the identification of individual proteins associated with black point. Two-dimensional gel electrophoresis was used to compare the proteome of the husk and whole grain tissue of mature black pointed and healthy grain. Very little watersoluble protein was extracted from the husk tissue. However, a significantly larger amount of protein was extracted using a salt extraction buffer, indicating the husk proteins were mostly cell wall bound. Due to the effect of residual salt and low protein concentrations these proteins were not conducive to analysis using two-dimensional gel electrophoresis. Further experiments using acid hydrolysis of the husk tissue and subsequent amino acid analysis revealed that the proteins were bound to the husk cell walls via covalent bonds. In contrast, large quantities of protein were obtained from the whole grain samples. This allowed statistically significant comparisons to be made between gels from healthy and black pointed grains. Two proteins were identified as being more abundant in black pointed grains. Mass spectrometry identified these as isoforms of barley grain peroxidase 1 (BP1). In addition, three proteins were identified as being more abundant in healthy grain. Mass spectrometry revealed these to be isoforms of the same protein with sequence similarity to a partial EST sequence from barley. Using 3' RACE the entire coding sequence of the gene was isolated which revealed that it encoded a novel putative late embryogenesis abundant (LEA) protein. Northern blot analysis was performed for BP1 and LEA and showed that gene expression differences could not account for the differences seen in protein quantities. Western blot analysis revealed that the LEA protein was biotinylated in vivo which is consistent with similar LEA proteins from other plant species. To further understand the role of these proteins in black point, antibodies were raised against the two proteins. Subsequent immunolocalisation studies indicated BP1 was present throughout all tissues of the grain whilst LEA was most abundant in the embryo and aleurone tissue. The second major area of investigation within this thesis was to further delineate the previously identified quantitative trait loci (QTL) associated with black point in barley. Previous studies have reported QTL for black point and kernel discolouration in both barley and wheat. Comparison of the published QTL revealed a locus on the short arm of chromosome 2H to be of particular interest. To identify genes underlying this QTL the genomes of barley, wheat and rice were compared. An in silico approach showed that the QTL shared macro-synteny with rice chromosomes 4 and 7. From the rice genome sequence, barley ESTs with sequence similarity were selected. In total, 20 ESTs were selected based on two main criteria: their putative role in black point and also being evenly spread across the region of the QTL length. These QTL were mapped within the Alexis x Sloop double haploid population. This approach revealed that there was some conservation of synteny but also identified clear boundaries where synteny between barley and rice had been lost since divergence. Significantly, the additional markers mapped to this region have enabled the initial black point QTL to be reduced from approximately 30cM to 20cM. In conclusion, this study has added significant knowledge our understanding of the genetics of black point in barley through the use of two approaches. The proteomics approach has aided in understanding the biochemical processes occurring within the grain in response to black point. The comparative genetics approach has aided in understanding the genetic control of an important region of the genome influencing black point susceptibility. Combined, these findings will direct future research endeavours aimed at producing black point resistant barley cultivars. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1323053 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2008
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