Apparent digestibility of forage soybean silage was evaluated utilizing a dual continuous flow apparatus in a Latin Square design. The fermenters were inoculated with a mixture of rumen fluid from two ruminally cannulated Angus cows. Fermenters were randomly assigned to one of the following treatments: 1) 76.1% alfalfa silage, 16.1% corn stover, 6.7% dry distiller’s grains (CON); 2) 75.1% soybean silage, 6.5% corn stover, 17.3 % soyhulls (SB); 3) 63.5% soybean pearl millet mixed silage, 15.2% corn stover, 20.2 % soyhulls (SBPM);4) 61.4 % rye grass, 14.8% corn stover, 4.8% dried distiller’s grains, 18.0 % soyhulls (RYE). All diets contained 1.1 % Purdue Heifer developer and were balanced to meet or exceed NRC requirements for a developing heifer and to contain 13.9% crude protein. Treatment had no effect (P ≥ 0.43) on the apparent ruminal digestibility of dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF) or crude protein (CP). The SB forage source had the greatest (P = 0.03) fat digestibility compared to SBPM but similar to CON and RYE. Data suggests that the digestibility of forage soybean silage is similar to the digestibility of alfalfa silage. Additionally, if a producer is interested in planting a mixture of forage soybeans and pearl millet silage it too is similar in digestibility when compared to forage soybean alone and alfalfa silage.

Master of Science / Department of Animal Sciences and Industry / Karol E. Fike / David M. Grieger / Synchronizing estrus before AI is an effective way to shorten the breeding season, and increasing the number of pregnancies per AI may lead to greater use and acceptance of synchronization protocols among beef producers. Our objective was to determine if pregnancy rates to fixed-time AI (FTAI) would be improved by delaying insemination in heifers not expressing estrus before FTAI in a 7-d CO-Synch + controlled internal drug release (CIDR) estrus-synchronization protocol. In Experiment 1, yearling beef heifers (n = 465) at three locations of commercial and purebred herds were treated with GnRH (Cystorelin 100 µg im) and a CIDR insert (1.38 g of progesterone) on Day 0. On Day 7 CIDR inserts were removed and all heifers received PGF[subscript]2α (Lutalyse 25 mg im) and were fitted with an estrus-detection patch (Estrotect; Rockway, Inc.). Heifers were assigned to three treatments based on estrus-detection patch color at 48 h after PGF[subscript]2α: (1). Estrus-Red 48 h (Red 48; n = 180), heifers that expressed estrus and were inseminated at 48 h; (2). Non-Estrus-Gray 48 h (Gray 48; n = 137) heifers that did not express estrus and were inseminated at 48 h; and (3). Non-Estrus Delayed- 56 h (Gray 56; n = 148), heifers that did not express estrus at 48 h, and were not inseminated until 56 h after PGF[subscript]2α. Pregnancy rate to AI was greatest (P < 0.0001) for Red 48 heifers (67.8%) compared with heifers in the Gray 48 (39.4%) and Gray 56 (42.6%) treatments. Heifers assigned to Gray 48 and Gray 56 achieved similar (P = 0.83) pregnancy rates. In Experiment 2, yearling beef heifers (n = 257) at two different locations were treated with the same 7-d CO-Synch protocol, but heifers were assigned to three different treatments based on estrus-detection patch color at 48 h after PGF[subscript]2α: (1). Estrus-Red 48 h (Red 48; n =95), heifers that expressed estrus and were inseminated at 48 h; (2). Non-Estrus-Gray 48 h (Gray 48; n = 84), heifers that did not express estrus but were inseminated at 48 h; and (3). Non-Estrus Delayed- 72 h (Gray 72; n = 78), heifers that did not express estrus at 48 h, and were not inseminated until 72 h after PGF[subscript]2α. Pregnancy rate to AI was greatest (P = 0.004) for Red 48 heifers (62.1%) compared with heifers in Gray 48 (40.5%), and Gray 72 (46.2%). No difference in pregnancy rates (P = 0.75) was detected between heifers assigned to treatments Gray 48 and Gray 72. Delaying insemination in heifers not expressing estrus by 48 h after PGF[subscript]2α did not improve pregnancy rates to AI.

Doctor of Philosophy / Department of Animal Sciences and Industry / Evan C. Titgemeyer / Two experiments were conducted to evaluate the effects of late gestation and early lactation on the nutritional status of beef heifers fed low-quality, warm-season grass hay. The first experiment compared DMI, DM digestibility, and ruminal dynamics of pregnant and lactating beef heifers to non-gestating, non-lactating heifers of a similar age and size. This study demonstrated that pregnant heifers ate less than non-pregnant heifers while maintaining similar digestibilities. Intake was similar between lactating and non-lactating, though DM digestibility increased postpartum in lactating heifers. Ruminoreticular fill was less for pregnant than for non-pregnant heifers; ruminoreticular fill was similar regardless of lactation status. Ruminal NH3 increased with increasing intakes throughout the study. Lactating heifers had less ruminal NH3 than non-lactating heifers. Total ruminal VFA concentration was similar from 10 wk prepartum through 10 wk postpartum except at 2 wk prepartum when gestating heifers had less total ruminal VFA concentration. The second experiment compared DMI, DM digestibility, passage rate, and plasma glucose and BHBA concentrations between pregnant heifers, pregnant cows, lactating heifers, and lactating cows which were fed low-quality, warm-season grass hay supplemented with 450 g/d of soybean meal. This study demonstrated that DMI increased with progressing gestation in heifers. Lactating heifers had greater intake than other groups postpartum. DM digestibility decreased with advancing gestation; gestating animals had greater digestibility than non-gestating animals. Lactation status did not influence DM digestibility, though lactating heifers had greater digestibility from 3 to 7 wk postpartum. Digestibility was not influenced by age. Pregnant animals had faster digesta passage rates than non-pregnant counterparts. Plasma glucose concentration increased during the prepartum period; pregnant and lactating animals had lesser plasma glucose concentrations than non-gestating, non-lactating animals. Plasma BHBA concentration was greater in pregnant and lactating animals than in non-pregnant and non-lactating animal; age was not an influence on BHBA concentration. Calves from mature cows grew faster than calves from heifers. These studies showed that beef heifers do not have the same patterns of intake as mature cows during late gestation. Heifers and their calves exhibited poorer performance when compared to mature cows when fed low-quality, warm-season grass hay.

The objectives of this study were to determine the effects of handling peri-puberal heifers for 2 h each week on in-chute behavior, isolation behavior, and the time required for each heifer to leave the testing area; and to determine if the location of the facial hair whorl was associated with any of the behavior scores or social dominance order. Crossbred beef heifers (n = 146) were assigned to be walked through, sorted and moved through a chute for 2 hr each wk for 20 wk (HANDLED) or allowed to remain on pasture unless handling was required to treat an injury or disease (CONTROL). In-chute behavior, isolation behavior and exit times were observed and scored at the beginning (0 wk), middle (10 wk) and end of the experiment (20 wk). The facial hair whorl on each heifer was classified as being high (above the eyes), middle (between the eyes), or low (below the eyes). At the end of the experiment pairs of heifers in the HANDLED group competed for a feed source and a social dominance order was estimated. Weekly handling decreased in-chute behavior scores of heifers with facial hair whorl positions classified as medium or low, but not in heifers that exhibited a hair whorl high on their face. Cattle in the HANDLED treatment group which had an initial isolation score of 2 or 3 had the greatest improvement in temperament over the entire experiment when compared to CONTROL animals with the same initial isolation score. The calmest heifers were not negatively affected by the handling, while the most agitated animals in the HANDLED had a similar overall change in isolation score as those animals in the CONTROL group. This indicates that while weekly handling improved the temperament and behavior of heifers with intermediate temperament rating at the outset of the experiment, weekly handling seemed unnecessary for the calmest heifers and did not have a beneficial effect on the heifers rated as the most nervous and agitated at the beginning of the experiment. Social dominance rankings were positively correlated (P < 0.10) with final in-chute behavior scores, but not with the other behavior scores or heifer body weight. Cattle with the hair whorls in the middle of the forehead had higher mean social dominant rank than those with hair whorls higher or lower on the face (P < 0.03). Overall, the results of this experiment indicate that behavior testing can reveal differences in the temperament of heifers and that, other than the most nervous and agitated heifers; repeated handling could serve to improve the temperament of the animals. / Master of Science