Two species of large carpenter bees (genus Xylocopa) were studied in southern Arizona. Nesting preference of X. c. arizonensis in floral scapes of Agave palmeri was found to depend on floral scape age and status of the thermal microenvironment. Evidence suggests that females assess scape age with extreme accuracy and this ability enables them to avoid those scapes that are degraded by other biota. Females also preferentially used Agave scapes that were shaded from the afternoon sunlight. Male mating behavior and lek site selection of X. varipuncta was also investigated near a large nesting site in a topographically flat area. Behaviors analyzed indicate that males tend to display singly and that they disperse pheromonal signals. Lek site selection was in accord with that found at three other study sites and further demonstrated that males did not clump their territories near nest sites. Distribution of male territories therefore does not appear to correspond to female distributions in the environment.

The honeybees Apis cerana and Apis mellifera are derived from the same ancestral base about two million years ago. With speciation and evolution, they have acquired many advanced living skills in common, but have also evolved very different living strategies due to different distributions. This thesis is an intensive study of the biology of the mixed-species colonies of these species, the aims of which were to investigate their behavioural relationships and uncover the evolutionary conserved features of their behaviours subsequent to speciation. The results show that the two species can form a stable society to perform normal tasks. First, workers of both species in the mixed-colonies could form the typical retinue behaviour to hetero-species queens, thus indicating that queen pheromones could be spread to and by both species. Secondly, both species did not show significantly different ovarian activation under hetero-species queens, suggesting that the queen pheromones more likely play a role of "honest signal" rather than a "repression" substance in the honeybee colonies. Thirdly, both species could mutually decode each other‘s waggle dances, with unexpectedly low misunderstanding; revealing that the dance language in a dark environment is quite adaptive for cavity-nesting honeybees. Fourthly, workers of both species could cooperate with each other in comb construction, although the combs they built contain many irregular cells. Interestingly, A. cerana workers could be stimulated by A. mellifera workers to perform this task, thus confirming self-organization theory in the colony. Fifthly, A. mellifera workers behaved more "defectively" in thermoregulation, but perhaps because A. cerana workers are more sensitive to changes in hive temperature. Given these differences in strategy, A. mellifera workers‘ performance might in fact reduce conflicts. Lastly, when faced with threats of predatory wasps, both species engaged in aggressive defence. Although they did not learn from each other‘s responses, species-specific strategies were adopted by each of them so that the defence of the mixed-colonies is very effective. I conclude that the two species can adapt to each other‘s efforts and task allocation is reasonably organized allowing mixed-species colonies to reach stability. These results suggest that all of the social behaviours discussed here were highly conserved following speciation. This thesis could provide some clues for the study of honeybee evolution from open-nesting to the transition of cavity-nesting.

Bumblebees have undergone dramatic declines both in Britain and further afield during the last century. Bumblebees provide a crucial pollination service to both crops and wild flowers. For these reasons, they have received a great deal of research attention over the years. However, the ecology of wild bumblebee nests and the interactions between nests and other species, particularly vertebrates has been somewhat understudied. This is largely due to the difficulty in finding sufficient nests for well replicated study and a lack of appropriate methods of observation. Here, methods for locating bumblebee nests were trialled. It was found that a specially trained bumblebee nest detection dog did not discover nests any faster than people who had received minimal instruction. Numbers of nest site searching queens provide a reliable indication of suitable nesting habitat (i.e. places where nests are more likely to be found). In order to investigate aspects of bumblebee nesting ecology wild nests were observed by filming or regular observations by either researchers or members of the public. Some buff-tailed bumblebee (Bombus terrestris) nests were collected and all the bumblebees were genotyped to identify any foreign individuals. A review of British mammalian dietary literature was conducted to identify those that predate bumblebees. Great tits (Parus major) were filmed predating bumblebees at nests and it was clear from the literature and observations that badger (Meles meles), pine martens (Martes martes) and hedgehogs (Erinaceus europaeus) predate bumblebee nests, as well as the wax moth (Aphomia sociella). No evidence for predation by any other vertebrate species was found. Behaviours recorded included parasitism by Psithyrus, apparent nectar theft and possible usurpation by true bumblebees, egg-dumping by foreign queens and drifting and drifter reproduction by foreign workers. These events may cause harm to colonies (for example, through horizontal transmission of pathogens, or exploitation of the host nest’s resources). Alternatively where for example, usurpation by true bumblebees, egg-dumping or drifting is successful, these alternative reproductive strategies may increase the effective population size by enabling a single nest to produce reproductives of more than one breeding female. These data found that wild buff-tailed bumblebees (B. terrestris) nests with a greater proportion of workers infected with Crithidia bombi were less likely to produce gynes than those with fewer infected workers. Gyne production also varied dramatically between years. There is a growing body of evidence that a class of frequently used insecticides called neonicotinoids are negatively impacting bumblebees. An experiment was conducted using commercial colonies of buff-tailed bumblebees (B. terrestris) which were fed pollen and nectar which had been treated with the neonicotinoid imidacloprid at field realistic, sub-lethal levels. Treated colonies, produced 85-90% fewer gynes than control colonies. If this trend is representative of natural nests feeding on treated crops, for example, oilseed rape and field beans or garden flowers, then this would be expected to cause dramatic population declines. In this thesis methods for locating bumblebee nests have been tested, new behaviours have been identified (for example, egg-dumping by queens and predation by great tits) and estimations for rates of fecundity and destruction by various factors have been provided. Doubt has been cast over the status of some mammals as predators of bumblebee nests and estimates for gyne production, nest longevity, etc, have been given. More work is needed, especially observations of incipient nests as this is when the greatest losses are thought to occur.