Less than 1% of work and school trips are completed by bicycle in the United States. Comprehensive bicycle facilities improve bicycle ridership by including a diverse set of strategies that accommodate the bicycle mode and seek to minimize the Level of Traffic Stress experienced by riders. Traffic Signal Operational Strategies for Bicycles (TSOSB) are an integral component of comprehensive bicycle facilities. This research presents a methodology to identify critical zones for implementation of TSOSB. After identifying critical zones a process for assessment of gaps in bicycle safety and comfort and convenience for signalized intersections within the critical zones is conducted. The outcome of the methodology is a prioritized list of signalized intersection that could benefit from the application of Traffic Signal Operational Strategies for Bicycles