This study evaluated physical and biological treatments of bio-oil process water to decrease organic contaminants. A three-sequential-column filtration system compared four treatments: three columns filled with kenaf only; three columns filled with wood shavings only; first column filled with wood shavings and two with kenaf; and first column filled with kenaf and two with wood shavings. The kenaf and wood shavings were composted after filtration. The filtrate water underwent further bio-treatment by adding aeration and selected bacteria. After filtration and bio-treatment, oil and grease concentrations were reduced over 80 percent and toxicity reduced over 90 percent. There were no significant differences among filtration treatments. Most of the oil and grease was removed by the first column. Aeration significantly decreased the concentration of oil and grease and toxicity in the filtrate water. Composting of the bioiltration matrices significantly reduced the oil and grease concentrations at day 45 by 80 percent.