A associação da adubação nitrogenada com bactérias diazotroficas como o Azospirillum brasiliense pode favorecer o estabelecimento da planta em campo, além de constituir complemento nutricional para a planta de milho doce (Zea mays L. grupo saccharata). Os objetivos do trabalho foram: avaliar a interferência da inoculação de diferentes doses do produto comercial Azomax (Azospirillum brasiliense estirpes Ab-V5 e Ab-V6) na qualidade das sementes de milho doce, especialmente a germinação e o vigor e a interferência da inoculação no estabelecimento da planta em campo, avaliando desde a emergência da plântula até a maturação da planta, associada a adubação nitrogenada. As sementes foram inoculadas nas doses de 0 (controle), 2, 4 e 8 mL de Azospirillum/kg de sementes e em campo, combinadas a níveis de adubação nitrogenada de 80, 120, 160 e 200 kg de nitrogenio por hectare (blocos casualizados, esquema fatorial). A inoculação das sementes de milho doce com a bactéria Azospirillum brasiliense favoreceu o desenvolvimento radicular alterando o comprimento de raízes, diâmetro médio e massa de matéria seca; favoreceu o desenvolvimento da parte aérea proporcionando variações no teor de clorofila, diâmetro de colmo e teor de nitrogênio e apesar de não resultar em aumentos significativos da produtividade, influenciou no aumento do número de fileiras de grãos. A inoculação com Azospirillum por quilograma de não prejudicou a qualidade das sementes por até 60 dias após a inoculação. / The association of nitrogen fertilization with the inoculation of diazotrophic bacteria, such as Azospirillum brasiliense, might favor the plant establishment in the field and can work as a nutritional complement for sweet corn plants (Zea mays L. saccharate group). Taking from this combined action, the objective of this research was to evaluate the development and productivity of the sweet corn plant as a function of the inoculation and nitrogen fertilization in a randomized block design in a factorial scheme (4 x 4), 80, 120, 160 and 200 kg of nitrogen / ha) and 0, 2, 4 and 8 mL of Azospirilum / kg of seeds, with four replicates for each treatment. The inoculation of sweet corn seeds with Azospirillum brasiliense favored root development by increasing its length, diameter and amount of dry matter; favored the development of the plant by increasing content of chlorophyll, stem diameter and nitrogen content and despite not affecting significantly the yield of the plant increased number of grain rows. The application of Azospirillum have not caused negative effects on seed storage up to 60 days after inoculation.

The thesis is focused on biological seed treatment of selected field crops using adhesive in combination with entomopathogenic fungi Metarhizium anisopaliae and mycoparasitic fungi Trichoderma virens. Guar gum, xanthan gum and carboxymethylcellulose were chosen as the adhesive. Within the experiments in laboratory conditions, the percentage of kernels germination of spring barley, winter oilseed rape and a cucumber was evaluated after 24 hours and energy of germination and vitality during 2 5 days. The adhesive in combination with the filamentous fungi had positive influence on growth and development of embryonic roots of spring barley and growth and development of seedlings in all tested crops. The evaluation of plant emergence took place under laboratory and field conditions and the plants were biologically treated. Furthermore, the ability to colonize environment of the entomopathogenic fungus M. anisopliae was verified in this thesis. The fungus was injected into the substrate using seed dressings. According to the results, the introduced fungus is able to infect larvae Tenebrio molitor and Galleria mellonella after specific time.