Thesis (Ph. D.)--University of Illinois at Urbana-Champaign, 2006. / Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6238. Adviser: Archie R. Portis, Jr. Includes bibliographical references (leaves 138-154). Available on microfilm from Pro Quest Information and Learning.

Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2006. / "Title from dissertation home page (viewed July 11, 2007)." Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4208. Advier: Keith Clay.

Thesis (Ph. D.)--University of Illinois at Urbana-Champaign, 2006. / Source: Dissertation Abstracts International, Volume: 67-07, Section: B, page: 3551. Adviser: Evan DeLucia. Includes bibliographical references. Available on microfilm from Pro Quest Information and Learning.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. / Source: Dissertation Abstracts International, Volume: 67-07, Section: B, page: 3556. Adviser: Evan DeLucia. Includes bibliographical references. Available on microfilm from Pro Quest Information and Learning.

Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2006. / "Title from dissertation home page (viewed June 28, 2007)." Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 3000. Adviser: Roger P. Hangarter.

The overall objective of this study was to investigate the insecticidal and synergistic properties of an ethylacetate extract of Piper nigrum L. (Piperaceae) seeds used alone and in conjunction with pyrethrin, a botanical insecticide extracted from the flowers of Chrysanthemum cinerariaefolium Benth. & Hook. (Asteraceae). It was concluded that P. nigrum extract is a highly promising candidate for a novel botanical synergist for pyrethrin and could potentially be used as a replacement for piperonyl butoxide in certain pyrethrum formulations. The upregulation of mRNA transcripts encoding cytochrome P450 detoxification enzymes by a P. nigrum extract may indicate possible target proteins to enhance the toxicity of insecticides and synergists. The seven common genes differentially expressed in two or three of the sublethal treatments with pyrethrin, P. nigrum extract or pyrethrin plus P. nigrum extract merit further study of their functions relating to detoxification processes and defense responses. Future work should include a detailed, tissue-specific characterization of the genes of interest identified in this study and an investigation of the activity of the P. nigrum extract against the CYP enzymes associated with the Cyp genes that were differentially expressed in response to a P. nigrum extract. (Abstract shortened by UMI.)

A novel alteration in secondary metabolism of maize transformed with wheat oxalate oxidase gene (OXO) was determined using HPLC and microscopy. Phenolic concentration was significantly increased, but DIMBOA synthesis was down-regulated. The high levels of soluble phenolic acids, in particular free ferulic acid, most likely contributed to the insect resistance in the OXO maize. To facilitate future cowpea transformation with the OXO, a stable in vitro regeneration system was established in blackeye cowpea via shoot organogenesis. The optimal initiation medium comprised MS salts, B 5 vitamins, 2 mg 1-1 BAP, 3% sucrose and 0.8% agar at pH 5.8. The highest initiation frequency and shoot number were obtained from the shoot apices of 3-5 days old seedlings. For shoot elongation, 0.5-5.0 mg 1-1 GA3 was required. Rooting medium was MS salts supplemented with B5 vitamins, 3% sucrose and 0.8% agar.

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