<p> This thesis qualifies functional and ecological aspects of mucus trails deposited by <i>Elimia potosiensis</i> collected from the Meramec River. Freshwater snails are most often recognized for their significant role in river and stream ecosystem function as primary consumers and prey items. However, their form of locomotion is the most energetically expensive found in the animal kingdom. Found in large numbers and densities, <i> E. potosiensis</i> is capable of coating large swaths of substratum with carbohydrate- and protein-rich mucus. This has the potential to affect ecosystem function at the most basal trophic level both energetically, by contributing nutrients and energy to the surrounding water body, and functionally, as a sticky substance to which microheterotrophs become adhered. </p><p> In marine studies, researchers have found that bacteria can readily degrade gastropod mucus and that it can act as the basis for biofilm formation. There have also been studies that show unique employment of energy saving strategies by snails that use their trails to capture food items and to reduce production needs by following trails laid by conspecifics. Very little research has been conducted on freshwater gastropods. This study is the first to focus solely on the ecological and functional aspects of freshwater snail mucus. </p><p> For this study, collections of <i>E. potosiensis</i> were made from a single site along the Meramec River to qualify the effect deposited mucus has on the adherence of microalgae and its potential for formation of biofilms. Overall, mucus-coated surfaces significantly more particles in flow than non-coated surfaces. The water velocity at which a trail is laid was not found to have an affect on the thickness, adhesive ability or persistence of a trail. However, the water velocity in which a trail is incubated in stream does have effect on the adherence rate of a trail. In this study, it was found that this was most likely due to the fact that trails in high water velocity come into contact with more particles during a given period of time due, in part, to its higher carrying capacity.</p>

Expanding urbanization into undisturbed Sonoran Desert vegetation has led to public concern over the removal and destruction of large saguaro cacti (Carnegia gigantea). Development mitigation plans were established requiring transplantation, salvage and replacement of saguaros in certain development sites. However, no research has assessed whether native birds use transplanted saguaros at the same rate as non-transplanted cacti. The objective of this study was to determine the effects of saguaro transplantation on nest use. In Tucson, Arizona, 87 transplanted and 28 non-transplanted saguaros on a developed site and 26 non-transplanted saguaros on an undisturbed site were selected for observation of bird nesting activity during the breeding seasons of 1997 and 1998. Birds showed a preference for saguaros, transplanted or non-transplanted, which were surrounded by native vegetation and in areas with low human activity. I suggest that guidelines be revised for development mitigation standards and saguaro transplanting.

Ponderosa pine (Pinus ponderosa Laws.) forests have changed considerably during the past century, partly because recurrent fires have been absent for a century or more. In dense stands of ponderosa pine in the Black Hills of South Dakota, a layer of pine needles has replaced understory vegetation. I examined the disturbance history, soil seed bank, and effects of prescribed burning and overstory reduction on understory vegetation in a ponderosa pine stand in the northern Black Hills. Cessation of fires, prolific ponderosa pine regeneration, and logging led to a dense, even-aged stand with very little understory vegetation and few viable seeds in the soil seed bank. Understory vegetation did not respond to restoration treatments the first growing season, but did respond the second growing season. Paucity of viable seeds in the soil seed bank does not appear to constrain recruitment of understory vegetation in dense ponderosa pine forests of South Dakota.

A general paradigm in semi-arid and arid systems is that woody plants with dimorphic root systems will exhibit preferential use of deeper soil water because it represents a more stable source of water than short duration pulses of shallow soil moisture derived from summer rainfall. However, whether this holds across all woody species and whether use of deeper soil water interacts with use of shallow soil water is not determined for many species in different ecosystems. Understanding the amount of water plants derive from groundwater and shallow soil water is critically important to accurate calculations of local and regional water balance. The focus of this research was to determine if dominant woody species in semi-arid riparian ecosystems used shallow soil water and how depth to groundwater and defoliation might affect root proliferation and water uptake. This research found that the functional grouping "phreatophytes" encompasses a variety of responses to environmental variation. Stable isotopic analyses determined that Prosopis velutina Woot. (Velvet mesquite) and Populus fremontii Wats. (Fremont cottonwood) used shallow soil water derived from summer rainfall, and the proportion of shallow soil water was higher at sites with greater depth to groundwater. In contrast Salix gooddingii Ball (Goodding willow) did not use shallow soil water at any location regardless of depth to groundwater. Field experiments using defoliation treatments, to limit carbon assimilation and reduce plant photosynthate pools, confirmed that Prosopis velutina exhibited flexible response in water uptake patterns in response to defoliation. Defoliation, which presumably reduced available photosynthate, increased the reliance of this species on shallow soil water; contrary to predictions that woody species should maintain extensive deep root systems to buffer themselves from seasonal drought. Greenhouse experiments with Populus fremontii and Prosopis velutina also indicated changes in belowground biomass of fine roots, which were associated with changes in water-source use for Populus fremontii, but not for Prosopis velutina. These results imply that in terms of predicting plant response to changes in future climates, or modeling fluxes of water from the soil to the atmosphere that are largely controlled by plant transpiration, intra- and interspecific variability will need to be considered.

I studied bat activity at 13 artificial and 4 natural water sources in southern Arizona from May 1995 through August 1996 using mist-nets and ultrasonic detectors. I used 2 indices of bat activity, capture rates, calculated from mist-netting data, and bat passes/30 minutes, calculated from ultrasound data. Both methods provided similar information on temporal patterns of bat activity. Indices of bat activity were highest during the reproductive season (May through August) compared to the non-reproductive season (September through April). Bat activity did not differ between any of the 2-week time periods within the reproductive season. During the reproductive season, bat activity was bimodal with peaks during the first 2 hours after sunset and the 2 hours preceding sunrise. During the non-reproductive season, bat activity was unimodal, with the majority of activity occurred within 2 hours of sunset. I captured six bat species within the study area. Sex ratios tended to be male biased during the non-reproductive season but close to 1:1 during the reproductive season. Sex ratios did not differ between natural and artificial water sources for any of the bat species compared. Age ratios did differ significantly between natural and artificial water sites. A higher percentage of the total captures at natural water sources were juveniles compared to artificial water sources, possibly indicating higher reproductive output in areas surrounding natural sites. Indices of activity during the reproductive season were significantly higher at natural compared to artificial water sources. However, this difference was not due to higher insect biomass at natural water sources. I developed stepwise multiple regression models to explain variation in bat activity and species richness at water sources. Elevation, type of water source and distance to the nearest cliff or rock outcrop were significant variables in explaining variation in overall capture rates at water sources. Type of water source was the only significant variable explaining variation in ultrasonic activity (passes/30 min), while distance to the nearest source of permanent water and size of the water source explained 33% of the variation in species richness.

Fire scar analysis to identify fire events and stand age structure analysis to identify fire effects on survivorship of trees were used to reconstruct surface and crown fire regimes in upper elevation forests of the Gila Wilderness, NM. Fire regimes varied across forest type, but not necessarily across elevation. Prior to the twentieth century, (from 1706 to 1904), the mean return interval for large fires was 8 years. During the twentieth century, (from 1904 to 1995), the mean fire return interval for large fires was 46 years. The virtual end of historically frequent fire regimes due to livestock grazing and fire suppression since the turn of the century has affected successional pathways of forest types across elevations, favoring later successional forest species and structures.

Red-tailed hawks (Buteo jamaicensis), Swainson's hawks (Buteo swainsoni), great horned owls (Bubo virginiaus), and ravens (Covus corax and C. cryptoleucus) occur sympatrically in desert grasslands in Arizona. Desert grasslands have been invaded by trees since Anglo-American settlement. They may also be subject to greater human disturbance than historically, because of recent increases in illegal immigration along the U.S.-Mexico border. These changes may affect the abundance, diversity, competitive interactions, and reproductive success of raptors and corvids in desert grasslands. I studied raptors and corvids on the Santa Rita Experimental Range (SRER) and Buenos Aires National Wildlife Refuge (BANWR) in southern Arizona. My objectives were to (1) assess how spatial relationships among nesting raptors and corvids affect reproductive success, (2) identify characteristics of the environment selected by members of the assemblage for nest sites and home ranges, (3) identify similarities and differences in nest site and home-range characteristics among species, and (4) investigate how anthropogenic changes in desert grasslands have affected raptors and corvids. Productivity (x̄ [95% CI]) was high for red-tailed hawks on the SRER (1.82 [1.41, 2.23] young per breeding pair [YPB]), but low for all species on the BANWR (great horned owls: 0.96 [0.54, 1.38] YPB; ravens: 1.75 [1.39, 2.10]; red-tailed hawks: 1.04[0.83, 1.24]; Swainson's hawks: 0.91 [0.67, 1.16]) relative to range-wide averages (great horned owls: 1.42 [1.27, 1.56]; ravens: 2.39 [2.70, 3.07]; red-tailed hawks: 1.35 [1.20, 1.50]; Swainson's hawks: 1.34 [1.23, 1.45]). All species on both sites selected nest sites with taller nest trees and greater tree volume than available at random. Swainson's hawks selected home ranges with greater grass volume than available on BANWR. Overlap in habitat use was high among all species, exceeding 54% for nest trees, 80% for nest sites, and 90% for home ranges. Mesquite-dominated desert grasslands seemed to provide high quality habitat for red-tailed hawks on the SRER, but reproductive success for assemblage members on BANWR was poor. We suggest that competition, resulting from high habitat overlap, and human disturbance by illegal immigrants, has depressed raptor and corvid reproductive success on the BANWR.

The abundance, distribution, and diversity of the soil microbial community from a seawater-irrigated, halophyte agroecosystem was examined. The abundance and trophic composition of decomposers responded to the biochemical composition of residue from three salt-tolerant plants, Salicornia bigelovii, Suaeda sp., and Batis maritima. Decomposers were also influenced by the decomposition environment as it interacted with residue resource quality. The microbial community responded to the availability of residues and the planting density of the halophyte Salicornia bigelovii (Chenopodiaceae) in a season-long study at a research farm in Sonora, Mexico. Soil microbial biomass, activity and efficiency, as well as nematode abundance and trophic composition, generally increased in association with soil-incorporated halophyte residues. However, soil moisture and salinity, which decreased with depth, may also have been influential. Microbial biomass and activity responded to plant density only after plants entered senescence. This result suggested that live-root exudations were not available to the microbial community possibly due to competition with plant roots for available nutrient resources. An increase in the abundance of plant-feeding, but not bacterial- or fungal-feeding, nematodes was associated with the presence of S. bigelovii. The functional diversity of a microbial community from an agronomic halophyte system was compared to natural and constructed halophyte salt marsh communities. Functional diversity of the microbial community at the agronomic sites was intermediate compared to the other sites; e.g., higher than the constructed marsh but lower than the natural salt marshes. Relationships among the study communities determined by microbial functional diversity reflected both habitat and geographical influences.

It has been recognized that pollution (sulfate aerosols), and biomass burning (smoke aerosols), are the two principal anthropogenic sources that are now influencing the global climate. Researchers are currently trying to provide better estimates of the climate impact due to anthropogenic aerosols and to lower the range of uncertainty in their calculations. Estimations suggest a global-mean direct radiative forcing in the range from -0.3 to -1.0 W/m² for biomass burning aerosols, with a similar value for the indirect effect. The primary goal of this work is to estimate the global climate impact due to smoke aerosols from biomass burning using the National Center for Atmospheric Research (NCAR)-Community Climate Model, Version 3 (CCM3). Analysis of data from the AERONET project was used to define a more realistic average of the optical/radiative properties of smoke particles from biomass burning in the Amazon, and also, to provide relevant information about the cloud-aerosol interactions. This information was put into the context of the NCAR-CCM3 BATS, and then the cloud/radiative scheme of the standard model was modified to include both the direct and indirect effects of the aerosols. Our results suggest an annual global mean "total" (direct+indirect) radiative forcing of about -0.08 W/m². This value is in good agreement with recent estimations considering that we are only including the biomass burning aerosols in the Amazon region. In addition, our model simulations provide a "total" radiative forcing of about -10 W/m² for the Amazon region during the dry-season, where the indirect effect is responsible for about 80% of this value. This research hopefully will contribute to the aerosol climate modeling area by providing a scheme in which both the direct and indirect effects of the aerosol can be explicitly represented in a GCM.

The virile crayfish (Orconectes virilis), an aggressive polytrophic species, has been introduced into many Arizona streams. I investigated competition and predation between this crayfish and several native Arizona fishes. I conducted field experiments to assess competition for food between crayfish and fish, and laboratory experiments to examine competition for shelter and food, and predation. In Sabino Creek, I manipulated crayfish densities in isolated pools to examine effects of crayfish on growth, mortality, and recruitment of Gila chub (Gila intermedia). Regardless of crayfish density, Gila chub declined slightly in weight and condition. Mortality and recruitment did not differ between densities of crayfish. I examined crayfish effects on benthic macroinvertebrates, a submerged aquatic macrophyte and associated invertebrates, and three fish species in a small stream in the White Mountains by fencing eight stream sections to prevent movement. The three fishes were speckled dace (Rhinichthys osculus), Sonora sucker (Catostomus insignis), and desert sucker (C. clarki). Molluscs > 10 mm and macrophytes were less abundant at sites with a high density of crayfish than at sites with low crayfish densities. Insect diversity was lower in high- vs. low-density sites. No treatment effect was observed on growth or condition of individually marked fish. Short-term laboratory experiments demonstrated predatory interactions and competition for shelter between crayfish and Gila chub, desert sucker, and speckled dace. Crayfish used shelter more than fish, displaced fish from shelter, and frequently attacked fish. Fish never attacked crayfish, and only once displaced crayfish from shelter. In predation experiments, crayfish preyed upon all species, but preyed most heavily upon desert suckers. Fish never altered use of the water column in the presence of crayfish. Density manipulation experiments in a laboratory measured food competition between crayfish and two native fishes. Growth of Gila chub was less affected by crayfish than by increased density of Gila chub. Thus crayfish are not strong competitors with Gila chub for food. However, growth of flannelmouth sucker (Catostomus latipinnis) was negatively impacted by presence of crayfish. These laboratory experiments provide evidence that introduced crayfish can reduce fish growth by competition for food, and that native fishes are vulnerable to crayfish predation.