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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Neue Ansätze in der biomolekularen Interaktionsanalyse unter besonderer Berücksichtigung pharmazeutischer und lebensmittelchemischer Fragestellungen /

Hartmann, Markus. January 2004 (has links) (PDF)
Univ., Diss.--Bonn, 2004.
2

Modifizierte Polypyrrolfilme als Basis einer adaptierbaren Sensorarchitektur für reagenzlose Biosensoren

Habermüller, Katja-Carola. January 1999 (has links) (PDF)
Bochum, Universiẗat, Diss., 1999.
3

Real time monitoring of DNA hybridization and replication using optical and acoustic biosensors

Stengel, Gudrun. January 2004 (has links) (PDF)
Mainz, University, Diss., 2004.
4

Hydrophobin-Based Surface Engineering for Sensitive and Robust Quantification of Yeast Pheromones

Hennig, Stefan, Rödel, Gerhard, Ostermann, Kai 16 January 2017 (has links)
Detection and quantification of small peptides, such as yeast pheromones, are often challenging. We developed a highly sensitive and robust affinity-assay for the quantification of the α-factor pheromone of Saccharomyces cerevisiae based on recombinant hydrophobins. These small, amphipathic proteins self-assemble into highly stable monolayers at hydrophilic-hydrophobic interfaces. Upon functionalization of solid supports with a combination of hydrophobins either lacking or exposing the α-factor, pheromone-specific antibodies were bound to the surface. Increasing concentrations of the pheromone competitively detached the antibodies, thus allowing for quantification of the pheromone. By adjusting the percentage of pheromone-exposing hydrophobins, the sensitivity of the assay could be precisely predefined. The assay proved to be highly robust against changes in sample matrix composition. Due to the high stability of hydrophobin layers, the functionalized surfaces could be repeatedly used without affecting the sensitivity. Furthermore, by using an inverse setup, the sensitivity was increased by three orders of magnitude, yielding a novel kind of biosensor for the yeast pheromone with the lowest limit of detection reported so far. This assay was applied to study the pheromone secretion of diverse yeast strains including a whole-cell biosensor strain of Schizosaccharomyces pombe modulating α-factor secretion in response to an environmental signal.

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