1. The inheritance mechanism or resistance to lindane in the German cockroach, Blattella germanica (L.) was studied by determining the toxicity of lindane to the following populations ot cockroaches: a) lindane.resistant and non-resistant strains, b) the F1 and F2 progeny of reciprocal crosses between the two parental strains, c) the F1 and F2 progeny of backcrosses between the reciprocal cross F1 males. and resistant and non-resistant strain females, d) the progeny of pair matings within resistant and non-resistant strains and e) eight successive generation of a resistant strain isolated from further insecticide selection. 2. The method. employed in rearing the cockroaches and obtaining toxicological data on the different populations, as well as the genetical and statistical procedures, are described. 3. The toxicological data tor the mass matings are presented 1n the form of regression lines fitted by appropriate statistical analyses. 4. The toxicological data. for the pair matings, transformed into "indices of resistance," are presented in tabular form. 5.. Appropriate conclusions are drawn from the data to explain the inheritance mechanism of lindane resistance in the German cockroach. / Ph. D.

Dosage mortality data by a topical application method for eight organophosphorus compounds are given for two laboratory-selected .strains of Blattella p:ermanica ( L.), one resistant to malathion and the other resistant to diazinon. Comparison of this data with similar data for a normal susceptible strain of the same species reveals that development of resistance to malathion in this species is highly specific imparting no effective cross resistance to any of the other seven compounds evaluated. Selection with diazinon induces a low level of tolerance to all the organophosphates studied, but there are indications that in most cases this is due to vigor tolerance rather than to true resistance. There is reason to believe that if carboxyesterase activity is responsible for the resistance phenomena to malathion exhibited by this insect, its action is blocked or its detoxifying ability rendered ineffective by the presence of a vinyl group in an organophosphorus compound, such as GC 3707. / Master of Science

Made available in DSpace on 2014-06-11T19:25:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-25Bitstream added on 2014-06-13T20:08:03Z : No. of bitstreams: 1 parreira_rs_me_jabo.pdf: 339536 bytes, checksum: 9761fab34df675f3e45e2e4ae0a042ca (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo do trabalho foi avaliar a mortalidade de duas populações de Blattella germanica em função da área e do tempo de exposição a inseticidas. Os experimentos foram realizados no Depto. Fitossanidade, UNESP-Jaboticabal. Os inseticidas foram aplicados em torre de Potter, com volume de 1 mL sobre placas de Petri, em exposições de 2, 8 e 32 min, no primeiro experimento; e volume de 0,5 mL em áreas de exposição de 25, 50, 75 e 100%, no segundo experimento. No terceiro experimento foram tratadas superfícies revestidas por azulejos, com um pulverizador pressurizado (CO2). Foram utilizados os inseticidas gammacialotrina, bendiocarb, deltametrina, lambdacialotrina CS, lambdacialotrina CE, alfacipermetrina e cipermetrina nas dosagens recomendadas pelos fabricantes, comparados a uma testemunha sem aplicação. Foram confinadas cinco baratas adultas sobre a placa para os dois primeiros experimentos e dez baratas adultas nas superfícies de azulejo para o terceiro experimento. A mortalidade foi avaliada 0, 1, 2, 4, 24, 48 e 72 h após confinamento na superfície tratada. Verificou-se que os inseticidas foram eficientes para os três períodos de exposição. Para a área de exposição o inseticida cipermetrina apresentou a maior mortalidade que ocorreu na maior área. Na avaliação antes da lavagem os produtos resultaram em alta mortalidade, sendo a maior eficiência para o bendiocarb. Após a lavagem da superfície a maior eficiência foi do alfacipermetrina. A população de campo demonstrou ser mais resistente aos inseticidas do que a sensível. / This work goes evaluate the mortality of two Blattella germanica populations due insecticide treatments, treated area and exposition times. The experiments were in Dept. Fitossanidade at UNESP-Jaboticabal, SP, Brazil. The application of insecticides was realized in Potter’s tower over Petri plates with times exposition of 2, 8 and 32 min, to insects, spraying volume of 1 mL per plate; in another test it was evaluate exposition sizes of 25, 50, 75 and 100% areas to B. germanica and spraying volume of 1 mL per plate. Another application was over surfaces coverage with tile using a knapsack sprayer to test effect for washing in the tiles. For this, cockroaches (sensitive and field populations) were exposed before and after tiles had been washed. The products were: gammacyalotrine, bendiocarb, deltametrina, lambdacialotrina CS, lambdacialotrina CE, alfacipermetrina and cipermetrina, plus one check without application. Five cockroaches were confined over treated plates (first and second experiments) and ten over tile (third experiment). The mortality evaluation was 0, 1, 2, 4, 24, 48 and 72 h after cockroaches exposure. In the first experiment the insecticides was efficient to cipermetrina that promoted higher mortality. In relation of exposure periods, in all times it was observed high mortality of cockroaches. To areas, the higher was in the bigger area of exposure. In the third experiment, insects exposed before washing it was verify high mortality of cockroaches, mainly with bendiocarb, while after that the higher mortality was verified with alfacipermetrina. Field cockroaches survived more than sensitive population.

Resumo: O objetivo do trabalho foi avaliar a mortalidade de duas populações de Blattella germanica em função da área e do tempo de exposição a inseticidas. Os experimentos foram realizados no Depto. Fitossanidade, UNESP-Jaboticabal. Os inseticidas foram aplicados em torre de Potter, com volume de 1 mL sobre placas de Petri, em exposições de 2, 8 e 32 min, no primeiro experimento; e volume de 0,5 mL em áreas de exposição de 25, 50, 75 e 100%, no segundo experimento. No terceiro experimento foram tratadas superfícies revestidas por azulejos, com um pulverizador pressurizado (CO2). Foram utilizados os inseticidas gammacialotrina, bendiocarb, deltametrina, lambdacialotrina CS, lambdacialotrina CE, alfacipermetrina e cipermetrina nas dosagens recomendadas pelos fabricantes, comparados a uma testemunha sem aplicação. Foram confinadas cinco baratas adultas sobre a placa para os dois primeiros experimentos e dez baratas adultas nas superfícies de azulejo para o terceiro experimento. A mortalidade foi avaliada 0, 1, 2, 4, 24, 48 e 72 h após confinamento na superfície tratada. Verificou-se que os inseticidas foram eficientes para os três períodos de exposição. Para a área de exposição o inseticida cipermetrina apresentou a maior mortalidade que ocorreu na maior área. Na avaliação antes da lavagem os produtos resultaram em alta mortalidade, sendo a maior eficiência para o bendiocarb. Após a lavagem da superfície a maior eficiência foi do alfacipermetrina. A população de campo demonstrou ser mais resistente aos inseticidas do que a sensível. / Abstract: This work goes evaluate the mortality of two Blattella germanica populations due insecticide treatments, treated area and exposition times. The experiments were in Dept. Fitossanidade at UNESP-Jaboticabal, SP, Brazil. The application of insecticides was realized in Potter's tower over Petri plates with times exposition of 2, 8 and 32 min, to insects, spraying volume of 1 mL per plate; in another test it was evaluate exposition sizes of 25, 50, 75 and 100% areas to B. germanica and spraying volume of 1 mL per plate. Another application was over surfaces coverage with tile using a knapsack sprayer to test effect for washing in the tiles. For this, cockroaches (sensitive and field populations) were exposed before and after tiles had been washed. The products were: gammacyalotrine, bendiocarb, deltametrina, lambdacialotrina CS, lambdacialotrina CE, alfacipermetrina and cipermetrina, plus one check without application. Five cockroaches were confined over treated plates (first and second experiments) and ten over tile (third experiment). The mortality evaluation was 0, 1, 2, 4, 24, 48 and 72 h after cockroaches exposure. In the first experiment the insecticides was efficient to cipermetrina that promoted higher mortality. In relation of exposure periods, in all times it was observed high mortality of cockroaches. To areas, the higher was in the bigger area of exposure. In the third experiment, insects exposed before washing it was verify high mortality of cockroaches, mainly with bendiocarb, while after that the higher mortality was verified with alfacipermetrina. Field cockroaches survived more than sensitive population. / Orientador: Marcelo da Costa Ferreira / Coorientadora: Nilza Maria Martinelli / Banca: Marcos Roberto Potenza / Banca: Sérgio Antonio de Bortoli / Mestre