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Changes in plasma levels of non-esterified fatty acids (NEFA), glucose and L(+)-lactate in ; Beef cattle under feedlot conditions and background-age calves with respiratory disease ; Dairy cows from late pregnancy into early lactation / Changes in plasma levels on non-esterified fatty acids (NEFA), glucose and l(+)-lactate inOlumeyan, Daniel B January 2011 (has links)
Digitized by Kansas Correctional Industries
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Towards biomedical intelligent sensorsYalcinkaya, Fikret January 1998 (has links)
Potassium, sodium, and calcium are crucial electrolytes of human blood. Among the functions of the electrolytes are maintenance of osmotic pressure and water distribution in the various body fluid compartments, maintenance of the proper pH, regulation of the proper function of the heart and other muscles, involvement in oxidation-reduction or electron transfer reactions, and participation in catalysis as cofactors for enzymes. Therefore, abnormal levels of electrolytes may be either the cause or the consequence of a variety of disorders, and thus the determination of electrolytes in different body fluids in general and in whole human blood particularly is one of the most important functions of the clinical laboratory . Serum or plasma is the usual specimen employed for assay of potassium, sodium, and calcium but not whole human blood. This research intended to create a sensor-array capable of analysing potassium, sodium, and calcium ion-activity in whole human blood based on miniaturised ion-sensors and zero-current direct potentiometry. This research dealt with sensing-side of the sensor-array hoping for integration of these sensors with microelectronics or discrete parts based electronics in due time. Clinics use single electrodes for K+, Na+, and Ca2+ sensing in body fluids. However, single sensing is costly and in emergency cases time consuming as well. Therefore a portable, battery-operated cost- and time-effective sensor array is needed for multi-sensing of r, Na +, and Ca2 + either in emergency cases in field or for patient near bed-side measurement/monitoring. The approach of this thesis to the problem is as follows: This thesis has five phases, all equally important. The first phase, a theoretical phase, mainly deals with the determination of the technique which fits best with chemical sensors and integration with microelectronics, and the decision among many alternative chemicals, whether natural or man-made, available for multi-sensing realisation, by taking into account all the best efforts devoted to development of chemical-sensors. The second phase is the miniaturisation process of the electrochemical sensor-array, either ion-sensors or reference electrode, as much as possible. The third phase is multi-sensing application of the sensorarray for sensing K+, Na+, and ci+ with binary and mixed electrolytes and with artificial and whole human blood respectively, the fourth phase is the sensor-array response with only millilitre or microlitre volumes of whole human blood and the fifth phase is the integration of a sensor array with discrete, parts based, electronic circuitry. So, as a new application a disposable reference electrode has been realised which reasonably fits with the sensor array for the analysis of potassium, sodium, and calcium in whole human blood. Since all the single sensing of these cations have been realised by using a conventional reference electrode, either silver/silver chloride or calomel electrode, which is a bulky and expensive alternative, this research prepared, tested, and implemented a new disposable reference electrode for the sensor-array based on a poly (vinyl alcohol) matrix incorporating a proper amount of Kel. Having a successfully working miniaturised reference electrode, valinomycin, ETH 157, and ETH 1001 ionophores have been used as the selective materials for potassium, sodium, and calcium in whole human blood, respectively. Multisensing applications have only used solutions as testing medium whereas the work described in this thesis goes one step further and uses artificial and whole human blood with fairly reasonable responses.
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The phospholipids in human blood fractionsBetshart, Antoinette Alice 14 May 1966 (has links)
Although there is information available on the distribution of
phospholipids in human serum and red cells, very limited data have
been reported for white cells and platelets. To the knowledge of the
author, no data have been reported on the distribution of phospholipids
among the four blood fractions, of individual subjects.
Due to the limited amounts of white cells and platelets present
in blood, micromethods are essential for the analyses of these fractions
in individual subjects. Although phospholipids have been separated
from larger samples by others, their methods were not appropriate
for micro amounts. Therefore, procedures were developed in
this study which made it possible to isolate and to quantitate the individual
components of samples of total phospholipid ranging from
20 to 40 μg.
The distributions of phospholipids were determined in serum,
red cells, white cells and platelets isolated from the venous blood
of four men and four women. The blood fractions were isolated and lipid was extracted by methods previously developed in this laboratory.
Total phospholipids were isolated by preparative thin-layer
chromatography. Individual phospholipid components were separated
and quantitated by the micromethod developed in this investigation.
Components were eluted from microchromatoplates and quantitated
by analysis of their phosphorus content. This method effectively
separated phospholipid samples into lysophosphatidylcholine (LPhC),
sphingomyelin (Sph), phosphatidylcholine + phosphatidylserine
(PhC + PhS) and phosphatidylethanolamine (PhE).
Although all of the fractions contained higher proportional
amounts of PhC + PhS than any other component, there were characteristic
distributions in each of the blood fractions. Serum was characterized
by high PhC + PhS and virtually no PhE. Red cells contained
lower amounts of PhC + PhS and more Sph and PhE than any
other blood fraction. The distributions of phospholipids in white cells
and platelets were similar and resembled the general pattern found
in red cells more than that of serum.
The marked differences in the distribution of phospholipids among
the blood fractions emphasize the importance of concurrent analyses
of all blood fractions in studies of human phospholipid metabolism. / Graduation date: 1966
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Bovine plasma analysis for progesterone: a quantitative method employing gas liquid chromatographyGlenn, Lloyd Wayne, 1943- January 1967 (has links)
No description available.
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Variability of pesticides in human blood serumApple, Gail Illsley, 1941- January 1968 (has links)
No description available.
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Comparison of glycoproteins of blood from fish and mammals.Qureshi, Mohiy-Ud-Din. January 1970 (has links)
No description available.
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Change in blood PO[subscript]2 and PCO[subscript]2 with short storage timeJain, Anil K. 12 1900 (has links)
No description available.
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The lipids in human blood fractionsSmith, Elveda 23 October 1964 (has links)
Although much information is available on lipids in serum and
red cells, only limited data have been reported for white cells and
platelets. In a recent investigation in this laboratory, the concentrations
of cholesterol in the several blood fractions were compared.
The high amounts of cholesterol found in white cell-platelet samples
together with the wide variability in the proportions of free cholesterol
stimulated an interest to study in more detail the lipid composition
of the four blood fractions.
The concentrations of total lipids, the distributions of lipid
classes, and the fatty acid compositions of cholesterol esters, triglycerides,
and phospholipids were determined in serum, red cells,
white cells, and platelets isolated from venous blood obtained from
sixteen adult men and women. Supplementary analyses were made
on nine samples of blood from one person at intervals over a four-week
period.
Modifications of a published method made possible the simultaneous separation of each of the formed elements; serum was
obtained in the usual manner. Satisfactory procedures for total
lipid extraction from each blood fraction were developed by selective
combinations of individual steps of the classical ethanol-diethyl ether
and chloroform-methanol methods. The proportional distribution of
lipid classes in each blood fraction was quantitated by chemical analyses
of cholesterol and lipid phosphorus and subsequent calculations.
Lipid classes were isolated by preparative thin-layer chromatography.
Methyl esters of the major classes were prepared by direct interesterification
and analyzed by gas-liquid chromatography.
Concentrations of total lipid in sera and red cells were similar,
averaging 603 and 555 mg per 100 ml, respectively. White
cells and platelets contained two to three times as much, averaging
3051 and 2587 mg per 100 gm, respectively. The different blood
fractions exhibited characteristic distributions of lipid classes.
Serum was characterized by high cholesterol esters and triglycerides,
together with phospholipids. All cellular components contained
higher proportions of phospholipids and free cholesterol than
serum. White cells and platelets contained higher amounts of cholesterol
esters and free fatty acids than red cells. The fatty acid
compositions of cholesterol esters, triglycerides, and phospholipids
were not constant among the different blood fractions. The proportions
of fatty acids in triglycerides were most similar. Greatest variations in proportions of fatty acids occurred in cholesterol esters.
Lipid classes in all cellular fractions were characterized by containing
significant amounts of unidentified longer chain fatty acids which
were essentially absent in serum.
Individual-to-individual variations among the sixteen subjects
were greater than day-to-day variations of the one subject for total
lipids in serum and red cells, all lipid classes in serum, and phospholipids
in all blood fractions.
The concentrations of lipid in blood fractions showed no relationship
to the age or sex of the subjects or to their assessed intakes
of fat. Fat intakes were quite similar, however.
Differences in the distribution of lipid classes and fatty acid
composition of lipid classes among the four blood fractions for these
healthy subjects suggest the desirability of concurrent analyses of
all blood fractions in studies of lipid metabolism in humans. / Graduation date: 1965
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Concentrations of free and total cholesterol in human blood fractionsSmith, Elveda 11 May 1961 (has links)
Graduation date: 1961
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The relationship between anaerobic threshold, excess CO² and blood lactate in elite marathon runnersHearst, William Edward January 1982 (has links)
The purpose of this study was to investigate the use of excess CO₂ (ExCO₂) as a determinant of the anaerobic threshold
(AT) and the subsequent relationship to blood lactate (La). Four highly trained marathon runners (x values, age=30.6 years; % body fat= 8.2; VO₂max = 6 8 ml-kg⁻¹min⁻¹) volunteered to participate in this study. Metabolic and respiratory exchange variables were assessed by an open circuit method utilizing a Beckman metabolic measurement cart interfaced on-line with a Hewlitt Packard 3052A data acquisition system. VO₂max and the treadmill velocity at the threshold of anaerobic metabolism
(V[sub=tam]) were obtained from a progressive, treadmill run (.81 kph. >
min.) until volitional fatigue. V[sub=tam] (Kilometers per hour, Kph)
was calculated from the point of .a non-linear increase in ExCO₂.
Subjects performed set treadmill runs of 10 minutes on alternate
days. Variations (latin square) included runs at V[sub=tam], V[sub=tam+1], [sub=tam+2], and [sub=tam-1]. Analysis of variance with preplanned
orthogonal comparisons and Scheffe post hoc contrasts were
used to determine the effects of the treadmill variations on La
and ExCO₂. There was no significance found between V[sub=tam] and V[sub=tam-1] for La or ExCO₂. Significance (p < .05) was evident with V[sub=tam]< [sub=tam+1, V[sub=tam]< [sub=tam+2] for La and V[sub=tam]< [sub=tam+2] for ExCO₂.
An overall correlation of .89 (p <. 005) demonstrated a high
positive relationship between ExCO₂ and La. Findings indicate V[sub=tam]
to be a critical point in determining the anaerobic
threshold in marathoners, and performance above this demarcation results in a state of anaerobiosis. / Education, Faculty of / Curriculum and Pedagogy (EDCP), Department of / Graduate
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