Bloodstain pattern analysis is used in the investigation of a crime scene to infer the impact velocity and size of an impacting droplet and from these, the droplet’s point and cause of origin. The final pattern is the result of complex fluid dynamic processes involved in the impact and spreading of a blood drop on a surface with variable surface properties such as wettability and porosity. An experiment has been designed to study these processes and the resulting patterns for the case of a single Newtonian droplet impacting an inclined surface with variable roughness and wetting properties. An experimental apparatus, including a droplet generator, has been designed to produce droplets on-demand, and that impact an interchangeable surface. In addition, a blood-simulant liquid has been developed as a replacement for performing tests with real blood. With this apparatus and blood simulant, fluid dynamics concepts, such as contact line motion and wetting behavior are examined in the context of parameters of interest to the forensics community. These include eccentricity, spread factor and the number of spines formed on impact. The effect of varying dimensionless parameters including Reynolds number, Weber number and Laplace number, the angle of impact and surface properties is examined. Correlations are developed for predicting conditions at the point of impact from those observed later, as would be available to a forensics examiner, and the accuracy of the predictions developed in this thesis are evaluated.

The causes of errors in the angle of impact calculation were investigated including the surface type, falling velocity and the method used to fit an ellipse to a bloodstain. As had been cited previously the angle of impact was generally underestimated, especially at acute angles and the reason for this was determined to be due to an overestimation of the length of a bloodstain. The surface type was found to significantly affect the accuracy of an angle of impact calculation and as the falling velocity increased, the angle of impact calculation became more accurate. High-speed photography was used to further investigate the formation of bloodstains on surfaces. It was found that the formation of the bloodstain varied depending on the surface type and the angle of the surface. Bloodstain pattern analysis involves the application of scientific techniques to reconstruct events that resulted in a bloodstain pattern. The position of the blood source in three-dimensional space is a fundamental element of this application. Currently little is known about the methods used by bloodstain pattern analysts to select bloodstains when determining the region of origin. Fourteen analysts worldwide were surveyed in order to ascertain this information. It was found that the methods used were variable and were often not based on scientific research. Research was therefore undertaken into bloodstain selection and in particular, which bloodstains should be selected for a region of origin analysis. As a result of these experiments, two sets of selection criteria were established, one for use when the region of origin is being calculated manually and one for when directional analysis is being used.

The causes of errors in the angle of impact calculation were investigated including the surface type, falling velocity and the method used to fit an ellipse to a bloodstain. As had been cited previously the angle of impact was generally underestimated, especially at acute angles and the reason for this was determined to be due to an overestimation of the length of a bloodstain. The surface type was found to significantly affect the accuracy of an angle of impact calculation and as the falling velocity increased, the angle of impact calculation became more accurate. High-speed photography was used to further investigate the formation of bloodstains on surfaces. It was found that the formation of the bloodstain varied depending on the surface type and the angle of the surface. Bloodstain pattern analysis involves the application of scientific techniques to reconstruct events that resulted in a bloodstain pattern. The position of the blood source in three-dimensional space is a fundamental element of this application. Currently little is known about the methods used by bloodstain pattern analysts to select bloodstains when determining the region of origin. Fourteen analysts worldwide were surveyed in order to ascertain this information. It was found that the methods used were variable and were often not based on scientific research. Research was therefore undertaken into bloodstain selection and in particular, which bloodstains should be selected for a region of origin analysis. As a result of these experiments, two sets of selection criteria were established, one for use when the region of origin is being calculated manually and one for when directional analysis is being used.