Africa's miombo woodlands constitute one of the most important dry tropical forests on earth, yet the hydraulic function of these woodlands remains poorly researched. Given the current predictions of increased aridity by the end of this century in the miombo ecoregion, understanding the likely response of miombo woodlands tree species to water stress is crucial in planning adaptation strategies. Predicting the response of miombo woodlands to future climate trends is hampered by a lack of knowledge on the physiology of the common miombo woodlands tree species. In particular, plant-water relations for this woodlands type are not well understood. An understanding of plant-water relations for this woodlands type will provide insights into how water limits tree species distribution in this ecosystem. This will also improve our prediction model on the likely response of this ecosystem to predicted climate change. For this reason, the overall objective of this research was to evaluate the hydraulic architecture and xylem vulnerability to cavitation for nine principal miombo woodlands tree species differing in drought tolerance ability and habitat preference. This was achieved by; examining the hydraulic properties and evaluating the extent to which each hydraulic design was vulnerable to water stress-induced xylem cavitation; investigating how seasonal changes in plant-water relations influences seasonal patterns of leaf display and; analyzing the relationship between stem hydraulic supply and leaf functional traits related to drought tolerance ability. This research has found that drought-intolerant tree species with mesic specialization have more efficient stem hydraulic systems than co-occurring habitat broad ranging species. Broad ranging tree species attain wider habitat distribution by adjusting their hydraulic supply in response to changing ecosystem water availability. The finding that hydraulic properties differ significantly between tree species with contrasting habitat preference suggests that tree hydraulic design may have some adaptive ecological role in influencing species habitat preferences in miombo woodlands. The evaluation of xylem vulnerability to cavitation revealed that mesic specialized tree species were more vulnerable to water stress-induced cavitation than habitat broad ranging tree species. Vulnerability to cavitation in individuals from the same broad-ranging species growing in contrasting habitats showed only marginal and statistically insignificant (P > 0.05) differences between wet and dry sites. In the investigation of the influence of seasonal changes in stem water relations on seasonal leaf display, seasonal rhythms in stem water status were found to exert significant controls on leaf phenology. Mesic specialists had strong stem water controls throughout the year in comparison to broad ranging tree species. An analysis of the relationship between stem hydraulic supply and leaf functional traits suggests that stem hydraulic supply constrains leaf biomass allocation patterns among miombo tree species. Mesic specialists tend to invest more in leaf longevity than broad ranging tree species. This thesis has uncovered some interesting relationships between plant-water-relations and the distribution of miombo woodlands tree species. These results lead to the conclusion that in an event of increased ecosystem drying under future climate trends, tree species with mesic specialisation are at a greater risk of experiencing cavitation related species mortality than broad ranging ones.

Among the most compelling and least well-understood tree species growing in the temperate forests of southern South America is Fitzroya cupressoides, a high biomass species and the second longest-lived tree species in the world. This thesis quantified the main components of the carbon cycle in Fitzroya forests (i.e. net primary productivity (NPP) and soil respiration) and evaluated the environmental variables that are most related to them. The study was focused on medium-age and old-growth forests growing in the Coastal Range (Alerce Costero National Park, AC) and the Andean Cordillera (Alerce Andino National Park, AA) of southern Chile, respectively. The specific objectives of this thesis were to: 1) assess the forest structure, species composition and characterise the environmental conditions of these forests; 2) assess biomass, aboveground NPP, carbon allocation and mean wood residence time in these forests; 3) assess soil respiration and relate it to soil environmental conditions. Additionally, to use a mass balance approach to estimate fine root productivity; 4) estimate total NPP using biometric and indirect estimates of productivity; 5) evaluate the climatic factors mainly related to Fitzroya stem radial change on an intra-annual basis; and 6) evaluate changes in Fitzroya's tree growth and carbon isotopes during recent decades, and determine which environmental factors are more related to them. The last two objectives focus on Fitzroya as the dominant species and the subject of this study. Two 0.6 ha plots were installed within each national park; NPP was estimated for a year and soil respiration and high resolution stem growth measurements were monitored over almost two years. Aboveground biomass estimates for the Andean site are among the most massive reported in the world and carbon fluxes in Fitzroya forests are among the lowest reported for temperate wet forests worldwide. The longevity as well as the particularly rainy and nutrient poor soil conditions where these ecosystems grow may influence their exceptionally slow carbon dynamics. Differences in carbon fluxes between sites seem most probably driven by different environmental conditions rather than by developmental stage. Moreover, carbon fluxes were more sensitive to interannual climate variability in AC than AA. Warmer and drier summer conditions, likely to become more common under future climate change, more significantly affected stem growth and soil respiration in the Coastal Range than in the Andes. Regarding long-term changes, tree growth has been decreasing in the coastal site in the last 40 years and increasing in the Andes since the 1900s. These trends have been accompanied by an increase in intrinsic water use efficiency which is likely caused by rises in CO₂ and changes in climate conditions in both sites. Although Fitzroya grows in particularly wet and cool areas, projected drier and warmer conditions may have a negative effect on Fitzroya stem growth and carbon sequestration in both study sites. This effect would be more critical in the Coastal Range though, because of its more Mediterranean climate influence and more restrictive soil conditions in this area. Adequate resources are needed for the monitoring and conservation of these slow growth and massive forests especially in the Coastal Range, in order to avoid ongoing illegal cuttings and threatening forest fires.

The relative contributions of biome history and geological setting to historical assembly of species richness in biodiversity hotspots remain poorly understood. The tropical Andes is one of the world’s top biodiversity hotspots, and with its diverse biomes and the relatively recent but dramatic uplift, the Andes provides an ideal study system to address these questions. To gain insights into the historical species assembly of the tropical Andes, this study focuses on investigating patterns of plant species diversification in the Andean seasonally dry tropical forest (SDTF) biome. Three plant genera are used as study groups: Amicia (Leguminosae, Papilionoideae), Tecoma (Bignoniaceae), and Mimosa (Leguminosae, Mimosoideae). Species limits are re-evaluated to enable dense sampling of species and intraspecific diversity for phylogeny reconstruction for each group. Time-calibrated phylogenies for Amicia and Mimosa are presented and used to determine patterns of species diversification in time and space. For Tecoma, incongruence between nuclear and chloroplast gene trees precludes straightforward estimation of a species tree and this incongruence is attributed to possible reticulation caused by hybridization. Divergence time estimates and patterns of diversification for Amicia and Mimosa are compared with other Andean SDTF groups (Cyathostegia, Coursetia, Poissonia; Leguminosae) using isolation by distance and phylogenetic geographic structure analyses. Consistently deep divergences between sister species and high geographic structure across all five groups suggest that Andean SDTF lineages have persisted over the past 10 million years (My) with high endemism driven by dispersal limitation, caused by geographic isolation, following the most recent episode of rapid mountain uplift 5-10 My ago. This prolonged stasis of the Andean SDTF biome is in line with Miocene fossil and paleoclimate evidence. Finally, wider analyses of the contrasting evolutionary timescales of older SDTF and more recent high-altitude grassland diversity suggest that the exceptional plant species diversity in the Andes is the outcome of highly heterogeneous evolutionary histories reflecting the physiographical heterogeneity of the Andean biodiversity hotspot.

In a recent contribution in Nature (vol. 442, pp. 555-558) Austin & Vivanco showed that sunlight is the dominant factor for decomposition of grass litter in a semi-arid grassland in Argentine. The quantification of this effect was portrayed as a novel finding. I put this result in the context of three other publications from as early as 1980 that quantified photodegradation. My synopsis shows that photodegradation is an important process in semi-arid grasslands in South America, North America and eastern Europe.

A major goal in biology is to understand the genetic basis of morphological variation at different evolutionary scales, for example between and within species. Here I investigate this issue by using plant leaves as an example. Previously comparative studies between the simple leaf model plant Arabidopsis thaliana and its dissected leaf relative Cardamine hirsuta have shown that inter-specific differences in leaf shape mostly result from variation in local tissue growth and patterning (Vlad et al., 2014; Hay et al., 2006; Barkoulas et al., 2008). Here, I aim to elucidate the genetic basis of natural variation in leaf form within species, by using divergent strains of C. hirsuta. I present evidence that variation in six strains collected from geographically diverse locations results from different rates of progression of an age-dependent leaf development programme in a phenomenon known as heteroblasty. By using Quantitative trait loci (QTL) mapping with a recombinant inbred line (RIL) population derived from a cross between the Oxford and Azores strains, I detected six QTL that influence leaflet production on multiple leaves. A QTL located on the 4th linkage group was validated and selected for further analysis. Characterisation of QTL effect indicated that the QTL influences leaf form by altering the rate of heteroblastic development. Subsequently I fine mapped this QTL to a DNA segment of 48 kb containing the gene SQUAMOSA PROMOTER PROTEIN BINDING LIKE 9 (ChSPL9), a previously characterised regulator of age dependent development. The parental alleles of ChSPL9 show variation in their sequence and were transformed into A. thaliana to evaluate whether they contribute to the QTL effect. Resultant phenotypes mirrored the QTL effect suggesting that ChSPL9 does indeed contribute to this QTL effect. These results indicate that age-dependent leaf shape progression underlies variation in leaflet number within species and more broadly suggest that in the case of plant leaves different processes might underlie morphological variation between and within species.

Tropical forests have existed for up to one hundred million years, and today provide many ecosystem services vital for human well-being. They also harbour great biodiversity, which, in addition to its intrinsic value, plays a key role in the functioning of these ecosystems. Despite their local to global significance, there are still many knowledge gaps concerning the dynamic processes that govern the functioning of tropical forests. Rapid rates of deforestation and landscape conversion, predominantly for logging and industrial agriculture, are limiting the time and opportunity available to collect the information needed to fill these gaps. This research aims to shed light on the long-term ecological functioning of tropical forests, specifically investigating the history of disturbance in these ecosystems and the response of forest vegetation to past perturbations. The carbon-rich tropical peat swamp forests found along the coast of Sarawak, Malaysian Borneo, are a central focus of this study. For these forests in particular, a large deficit of knowledge surrounding their history and unique ecological functioning is coupled with some of the highest conversion rates of all tropical forest ecosystems across the world. In this thesis, palaeoecological data has been used to reconstruct temporal variability in forest vegetation coincident with external perturbations in order to identify changes in the resilience of these ecosystems through time, via indicators such as slowing rates of recovery and reduced regeneration of forest vegetation. Results suggest that tropical forest ecosystems have, for the most part, shown resilience to natural disturbances in the past, ranging from instantaneous localised tree-fall to longer-term regional climatic change; but that recent anthropogenic disturbances, of novel forms and greater intensities, are jeopardizing the potential for forest recovery and thus compromising ecosystem resilience. These findings enhance our understanding of the ecology of tropical peat swamp forests, and tropical forests more broadly. They also provide a context for contemporary tropical forest management, allowing for predictions on future responses to disturbance and enabling more ecologically sustainable landscape planning.

Small Island Developing States (SIDS) of the Caribbean are one of the world’s ‘hottest’ ‘biodiversity hotspots’. However, this biodiversity continues to be threatened by habitat loss, and now, by climate change. The research reported here investigated the potential of species distribution modelling (SDM) as a plant conservation tool within Caribbean SIDS, using Trinidad as a case study. Prior to the application of SDM, ancillary analyses including: (i) quantification and mapping of forest cover change (1969 to 2007) and deforestation rates, and (ii) assessment of the island’s vegetation community distribution and associated drivers were carried out. Community distribution and commercial importance and global/regional rarity were used to generate a list of species for assessing the potential of SDM within Trinidad. Species occurrence data were used to generate species distribution models for present climate conditions within the SDM algorithm, MaxEnt. These results were assessed through expert appraisal and concurrence with results of ecological analyses. These models were used to forecast suitable species climate space forty years into an SRES A2 future. Present and future models were then combined to produce a ‘collective change map’ which showed projected areas of species’ range expansion, contraction or stability for this group of species with respect to Trinidad’s Protected Areas (PAs) network. Despite the models being indicative rather than accurate, it was concluded that species’ climate space is likely to decrease or disappear across Trinidad. Extended beyond Trinidad into the remainder of the Caribbean region, SDM may be a crucial tool in identifying which PAs within the region (and not individual islands) will facilitate future survival of given target species. Consideration of species conservation from a regional, rather than an individual island perspective, is strongly recommended for aiding the Caribbean SIDS to adapt in response to climate change.