The subject of diploma thesis is designing multi-span highway bridge over Řepovský potok valley, dirt roads and biocorridor. Three variants of bridge were designed and one of them – box girder 10 span bridge, has been chosen. Bridge deck is made from cast in place post tension prestressed beam. Load-bearing structure is concreting step by step on fixed and moving support. Total length of bridge is 560 m. The analysis of structure was projected on several computational models. They have been made in software Midas Civil and SCIA Engineer 18.1. The assessments of ultimate limit state and serviceability limit state were made in all steps of building in longitudinal and cross direction. In the analysis in longitudinal direction is included the time dependent analysis. Structural design was made in software MS Excel according to valid standards. Finally, drawing documentation and visualization were made.