Spelling suggestions: "subject:"branched"" "subject:"tranched""
21 |
Peptide nanovesicles: supramolecular assembly of branched amphiphilic peptidesGudlur, Sushanth January 1900 (has links)
Doctor of Philosophy / Department of Biochemistry / John M. Tomich / Peptide-based delivery systems show great potential as safer drug delivery vehicles. They overcome problems associated with lipid-based or viral delivery systems, vis-a-vis stability, specificity, inflammation, antigenicity, and tune-ability. We have designed and synthesized a set of 15 and 23-residue branched, amphiphilic peptides that mimic phosphoglycerides in molecular architecture. They undergo supramolecular self-assembly and form solvent-filled, bilayer delineated spheres with 50-150 nm diameters (confirmed by TEM and DLS). Whereas weak hydrophobic forces drive and sustain lipid bilayer assemblies, these structures are further stabilized by β-sheet hydrogen bonding and are stable at very low concentrations and even in the presence of SDS, urea and trypsin as confirmed by circular dichroism spectroscopy. Given sufficient time, they fuse together to form larger assemblies and trap compounds of different sizes within the enclosed space. They are prepared using a protocol that is similar to preparing lipid vesicles. We have shown that different concentrations of the fluorescent dye, 5(6)-Carboxyfluorescein can be encapsulated in these assemblies and delivered into human lens epithelial cells and MCF-7 cells grown on coverslips. Besides fluorescent dyes, we have delivered the plasmid (EGFP-N3, 4.7kb) into N/N 1003A lens epithelial cells and observed expression of EGFP (in the presence and absence of a selection media). In the case of large molecules like DNA, these assemblies act as nanoparticles and offer some protection to DNA against certain nucleases. Linear peptides that lacked a branching point and other branched peptides with their sequences randomized did not show any of the lipid-like properties exhibited by the branched peptides. The peptides can be chemically decorated with target specific sequences for use as DDS for targeted delivery.
|
22 |
The Influence of Chronic Branched-Chain Amino Acid Supplementation on Measures of Central and Peripheral Fatigue in Training AthletesWhiton, Tara K. 01 August 2019 (has links)
Branched-chain amino acid (BCAA) supplementation may improve recovery from competitive endurance training by reducing symptoms of central and peripheral fatigue. The purpose of this dissertation was to provide evidence for the use of BCAAs as a training nutrition strategy in order to improve recovery from training and further drive adaptive processes to training by increasing readiness to train. Collegiate distance runners undergoing intense competitive endurance training were monitored for symptoms of muscular soreness and psychological stress through a rated soreness chart and the Daily Analysis of Life Demands for Athletes Survey (DALDA) while taking either a BCAA supplement or a placebo. When on the BCAA supplement, athletes reported significantly fewer symptoms of psychological stress and reduced muscular soreness. These results point to the importance of nutrient bioavailability, specifically BCAAs, on recovery parameters when undergoing intensive training. This concept was also demonstrated in a case study on a trained distance runner who underwent intensive training for an ultra-endurance marathon. Running kinematics were assessed using Kinovea open-sourced software (Version 0.8.15) during a series of constant-paced endurance runs while on the BCAA supplement or a placebo. We observed a reduction in vertical oscillation when the runner was on the BCAA supplement, indicating improved muscle recovery and therefore efficiency of movement. Improving recovery by reducing global central and peripheral fatigue symptoms may increase readiness to train and further promote desired training adaptations.
|
23 |
Branched Covering Constructions and the Symplectic Geography ProblemHughes, Mark Clifford January 2008 (has links)
We apply branched covering techniques to construct minimal simply-connected symplectic 4-manifolds with small χ_h values. We also use these constructions to provide an alternate proof that for each s ≥ 0, there exists a positive integer λ(s) such that each pair (j,8j+s) with j ≥ λ(s) is realized as (χ_h(M),c_1^2(M)) for some minimal simply-connected symplectic M. The smallest values of λ(s) currently known to the author are also explicitly computed for
0 ≤ s ≤ 99. Our computations in these cases populate 19 952 points in the (χ,c)-plane not previously realized in the existing literature.
|
24 |
Branched Covering Constructions and the Symplectic Geography ProblemHughes, Mark Clifford January 2008 (has links)
We apply branched covering techniques to construct minimal simply-connected symplectic 4-manifolds with small χ_h values. We also use these constructions to provide an alternate proof that for each s ≥ 0, there exists a positive integer λ(s) such that each pair (j,8j+s) with j ≥ λ(s) is realized as (χ_h(M),c_1^2(M)) for some minimal simply-connected symplectic M. The smallest values of λ(s) currently known to the author are also explicitly computed for
0 ≤ s ≤ 99. Our computations in these cases populate 19 952 points in the (χ,c)-plane not previously realized in the existing literature.
|
25 |
Branched-chain amino acid nutrition and respiratory stability in premature infants /Nelson, Christy L. January 2002 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 2002. / "December 2002." Typescript. Vita. Includes bibliographical references (leaves 202-211). Also available on the Internet.
|
26 |
Removal of Perfluorooctane Sulfonate (PFOS) and Related Compounds From Industrial EffluentsOchoa-Herrera, Valeria Lourdes January 2008 (has links)
Perfluorooctane sulfonate (PFOS) and related perfluoroalkyl surfactants (PFAS) are ubiquitous contaminants of increasing public concern due to their environmental persistence, toxicity, and bioaccumulation. These perfluorinated compounds have been used for more than half a century in a wide variety of industrial and consumer products ranging from stain repellents such as Teflon® to aqueous fire-fighting foams and to grease-proof food packing. The public health and environmental risks posed by PFAS have driven environmental agencies and the industry to restrict their use to specific applications where they cannot be replaced by other chemicals. The sources and pathways of PFOS and its derivatives in the environment are not well understood. Analysis of environmental samples is critical to understand the fate, transport and persistence of these emerging contaminants. Techniques based on fluorine nuclear magnetic resonance (¹⁹F NMR) spectroscopy and high performance liquid chromatography (HPLC) with suppressed conductivity detection were successfully developed to monitor the presence of PFAS in water samples. Chromatographic separation of C₄ to C₈ PFAS surfactants was achieved using a C₁₈ reversed-phase column and a mobile phase consisting of a mixture of boric acid and acetonitrile at mixing ratios ranging from 75:25 to 45:55 (v/v). The combination of these two techniques was very effective for characterization and routine quantification of PFOS and related chemicals. Analytical methods based on ¹⁹F NMR, HPLC-suppressed conductivity detection, and liquid chromatography with tandem mass spectrometry (LC-MS/MS) were employed to characterize commercial PFOS samples. Linear and branched PFOS isomers in a percentage ratio of 75:25 were identified. Municipal wastewater treatment systems are one of the major sources of PFAS emissions into the environment. The presence of PFAS in sewage sludge from two wastewater treatment plants in Tucson, Arizona, was investigated. Sludge samples were washed with acetic acid and extracted with a mixture of acetic acid and methanol. The extract was cleaned and concentrated by means of solid phase extraction. LC-MS/MS operating in the selective ion monitoring (SIM) mode was employed to assess the presence of perfluorosulfonates, perfluorosulfonamides, and perfluorocarboxylates in sewage sludge samples. PFOS was the only perfluoroalkyl chemical detected in municipal sludge samples at a concentration of 77 ± 5 g kg⁻¹ sludge dry weight. Cost-effective treatment techniques for removing PFAS from industrial effluents are needed to minimize discharges of these pollutants. Reductive dehalogenation is widely applied to the degradation of highly chlorinated compounds. Hence, the susceptibility of PFOS and related compounds to biological and chemical reductive dehalogenation was evaluated in batch assays. PFAS were not reductively dehalogenated by different microbial consortia even after periods of incubation exceeding 2 y, confirming the high resistance of these compounds to microbial degradation. The anaerobic biodegradability of PFOS and perfluorobutane sulfonate (PFBS) samples exposed to electrochemical pretreatment with boron-doped diamond film electrodes was also investigated. The oxidation decreased the concentration of PFAS and dissolved organic carbon in solution, confirming the destruction of these compounds. However, the oxidative treatment did not enhance the susceptibility of PFAS to microbial degradation even after extended periods of incubation (> 1 y). In contrast, PFOS was reductively dehalogenated with a biomimetic system based on vitamin B12 as the catalyst and Ti(III) citrate as the reducing agent. The optimal treatments conditions of the reaction were 260 μM vitamin B₁₂, 36 mM Ti(III) citrate, 70°C and solution pH 9.0. Interestingly, branched PFOS isomers were more prone to degradation by vitamin B₁₂ catalysis compared to the linear isomer. Removal of 3 mol Fper mol of technical PFOS and 12 mol F- per mol of branched PFOS isomers was achieved. Defluorination of PFOS was also observed at environmental relevant conditions of 30°C and pH 7.0, albeit at lower degradation rates. Fluoride and carbon dioxide were identified as the major products of the chemical defluorination. Traces of partially fluorinated volatile compounds were also detected in the headspace. The feasibility of removing PFAS compounds from aqueous streams by sorption onto granular activated carbon (GAC), zeolite, and wastewater treatment sludge was examined in batch isotherm experiments. The fluorocarbon chain and the functional group influenced sorption of the anionic surfactants, PFOS adsorbed more strongly to GAC than perfluorooctanoic acid (PFOA) and PFBS. Activated carbon showed the highest affinity for PFOS (Freundlich K(F) values of 36.7 to 60.9) followed by the hydrophobic, high-silica zeolite NaY (Si/Al 80, K(F) of 31.8) and lastly anaerobic sludge (K(F) of 0.95 to 1.85). GAC sorption is a suitable treatment for the removal of anionic perfluoroalkyl surfactants when present at low concentrations. Fluoride has been identified as the major product of the reductive dehalogenation of PFOS and derivatives. Thus, the toxicity of inorganic fluoride towards the main microbial populations responsible for the removal of organic constituents and nutrients in wastewater treatment processes was also studied. Fluoride concentrations ranging from 18 to 43 mg L⁻¹ caused 50% inhibition (IC₅₀) of the activity of propionate- and butyratedegrading microorganisms and of acetate-utilization by methanogens evaluated under mesophilic and thermophilic conditions. All other microbial populations evaluated in this study, i.e., glucose fermenters, aerobic glucose-degrading heterotrophs, denitrifying bacteria, and H₂-utilizing methanogens tolerated fluoride at very high concentrations (> 500 mg L⁻¹). In the same manner, H₂-utilizing methanogens also tolerated PFOS and PFBS at concentrations as high as 200 and 500 mg L⁻¹, respectively.
|
27 |
Expression studies on the shortbranched chain acyl-CoA dehydrogenase (SBCAD) geneVicanek, Caroline Michaela January 1995 (has links)
Short/branched chain acyl-CoA dehydrogenase (SBCAD), a member of the acyl-CoA dehydrogenase (ACD) family of enzymes, catalyzes the oxidation of branched chain fatty acids and the branched chain amino acids isoleucine and valine. This research project focuses on expression studies of the SBCAD gene. Northern blot analysis detected two SBCAD mRNA species of 2.7 and 6.5 kb in various human tissues and cell types. A single 4.1 and 2.0 kb SBCAD message was detected in rat and pig tissues, respectively, revealing a species difference in SBCAD mRNA size. Studies of human and rat SBCAD tissue-specificity and relative abundance, at both the RNA and protein levels, identified liver and kidney as the tissues with the highest levels of SBCAD expression, establishing a unique tissue-specific expression pattern that is not seen among the other members of the ACD family. Furthermore, a fetal and adult difference in SBCAD expression was observed in human kidney, suggesting that the SBCAD gene may be developmentally regulated in some tissues. Finally, an attempt was made to isolate and characterize the SBCAD promoter region in order to provide valuable data for future SBCAD promoter studies.
|
28 |
The effects of branched-chain amino acid and leucine ingestion on the ERK1/2 MAP kinase signal transduction pathway in conjunction with an acute bout of heavy resistance exerciseCampbell, Bill. Willoughby, Darryn Scott, January 2007 (has links)
Thesis (Ph.D.)--Baylor University, 2007. / Includes bibliographical references (p. 134-146).
|
29 |
The effects of heavy resistance exercise in combination with orally administered branched-chain amino acids or leucine on insulin signaling and Akt/mTOR pathway activity in active malesLa Bounty, Paul. Willoughby, Darryn Scott, January 2007 (has links)
Thesis (Ph.D.)--Baylor University, 2007. / Includes bibliographical references (p. 145-152).
|
30 |
The effects of branched-chain amino acid supplementation on indirect indicators of muscle damage and performanceGreer, Beau Kjerulf. Haymes, Emily M., January 2006 (has links)
Thesis (Ph. D.)--Florida State University, 2006. / Advisor: Emily Haymes, Florida State University, College of Human Sciences, Dept. of Food, Nutrition, and Exercise Sciences. Title and description from dissertation home page (viewed Sept. 19, 2006). Document formatted into pages; contains xi, 123 pages. Includes bibliographical references.
|
Page generated in 0.0527 seconds