In broiler operations, various health problems develop during the final two weeks of the growing period, resulting in increased mortality and condemnation losses. At this stage, sickly birds were found to be systemically infected by various bacteria regardless of varied clinical signs. The main objective of the present study was to determine the prevalence and nature of systemic bacterial infections in unthrifty commercial broiler chickens and to establish a reproducible infection model in the laboratory. Thirty-one unthrifty 6-week-old broilers were obtained from three farms, and bacterial isolations were conducted on blood, liver, and hock joint. Bacteria were isolated from 87, 90, and 71% of the blood, liver and hock joint samples, respectively. Mean bacterial counts (log������ CFU/ml or g) of the blood and liver were 2.15 and 2.93, respectively. Among 132 bacterial isolates, major species were; Staphylococcus (60%), Corynebacterium (18%), Escherichia coli (5%), and Stomatococcus (4%). Among 79 Staphylococcus isolates, 77 were coagulase-negative. Major species of staphylococci were; S. lentus (19%), S. simulans (18%), S. cohnii (13%), S. gallinarum (10%) and S. captis (7%). In addition, 6 species of gram-positive and 5 species of gram-negative organisms were isolated. Apparently systemic infections were not caused by predominant pathogenic bacterial species, and adequately described as mixed infections. However, there were some significant relationships between isolated bacterial species and sampling sites, suggesting that certain organisms were abundant in the environment of a particular poultry house. These results indicate that systemic infections in market age broilers are caused by mixed bacterial species and suggest that they are caused by suppressed host antibacterial systems rather than pathogenic factors of microorganisms. Antibiotic susceptibility results showed 100% susceptibility of staphylococcal isolates (n=69) against vancomycin and enrofloxacin. Of these coagulase negative staphylococci showed 19% and 73% resistance against methicillin and penicillin G, respectively. There was also heterogeneity in antibiogram profiles within species of coagulase-negative staphylococci. Pathogenicity of representative field isolates from the above described study was tested in 5-day-old embryonated eggs and in 3- week-old broiler chicks. Consistent lethality was demonstrated with S. aureus in embryos. Staphylococcus intermedius or S. lentus demonstrated some pathogenicity, while S. gallinarum or Corynebacterium were non-pathogenic in embryos. In 3-week-old broilers, however, only S. aureus caused septicemia and death; other bacterial species mentioned above caused neither clinical signs of acute or chronic staphylococcosis nor mortality. / Graduation date: 1998

Seven experiments were carried out to investigate the involvement of carbohydrate and lipid metabolism on the incidence of Sudden Death Syndrome (SDS) in broiler chickens. Hepatic arachidonate level decreased after 1 h postmortem. The decreased level of hepatic arachidonate previously observed in SDS birds was, therefore, associated with the post-mortem aging. Broiler chickens fed diets high in glucose monohydrate (cerelose) were more susceptible to SDS mortality than broilers fed diets high in corn starch or corn-soy. Feeding broilers a high cerelose diet increased the levels of plasma calcium, total protein, triacylglycerol, and uric acid compared to broilers fed corn starch diet. Broilers fed cerelose diet also showed the higher incidence of leg abnormality than broilers fed corn starch or corn-soy diets. Daily intraperitoneal injection with 0.25 ml of L(+) lactic acid solution (100 mg lactic acid/ml) to broilers over a 7-d period failed to reproduce the SDS incidence, whereas intravenous injection of 40% lactic acid solution (200 mg lactic acid/kg body weight) resulted in 100% incidence of SDS-type mortality. No SDS-type mortality was observed with the intravenous injection of 40% sodium lactate solution (200 mg sodium lactate/kg body weight). Disturbance of physiological acid-base balance might be a factor in the SDS-type mortality. Suboptimal thiamin level in broilers fed cerelose diets was observed. Thiamin supplementation to cerelose diet improved the thiamin status of the broilers. Mortality due to SDS was decreased when thiamin hydrochloride was supplemented to cerelose diet at the level of 0.6 and 2.8 mg thiamin hydrochloride/kg diet, respectively. Thiamin supplementation, however, did not change the liver pyruvate dehydrogenase activity and the proportion of pyruvate dehydrogenase in the active form. Disturbance of acid-base balance was postulated to be associated with the incidence of SDS. Other genetic, nutritional, and environmental factors are likely to modify the incidence by affecting the acid-base status of the chicken. / Graduation date: 1993

Thesis (MPhil (Animal Sciences))--University of Stellenbosch, 2006. / Rickets is described as a disease that affects young growing poultry. Poorly mineralized bones with thickened and irregular growth plates characterize it. The onset of rickets is characterized by a failure of mineralization of cartilage and bone. Other symptoms of rickets include reluctance to movement in affected birds. These birds will sit on their hocks and if startled they use their wings for balance. On necropsy, bones are soft and fragile and they have thickened growth plates. In this study 30% of the chicks aged between 7 and 8 days from a broiler flock, started showing splay leg problems. Affected chicks were unable to support their weight on their legs, some showing paralysis. The bones were soft and rubbery. To try and identify the possible cause, bones from the affected chicks were analyzed for calcium (Ca) and phosphorus (P) to determine the Ca:P ratio. Blood serum was also analyzed for the mineral content. Ca and P were the main focus of the tests as the problem was suspected to be rickets. The feed was analyzed for protein, Ca and P. The bone analysis showed a Ca:P ratio of less than 2:1, while results of the blood serum showed an inverse Ca: P ratio. The analysis results of the feed as well as the bones showed an imbalance in the Ca:P ratio which according to literature and research done is a possible cause for rickets. These findings combined with the symptoms displayed by the affected birds, lead to the suggestion that the problem in this study was rickets.