This thesis experimentally investigates the effects of buffer regulation on the delivery of randomly spaced objects through a multi-conveyor system according to a demanded throughput and spacing. A regulator is developed and tested in conjunction with on ongoing research project at Georgia Tech investigating the automated transfer of live birds. In this thesis, an algorithm is proposed to identify and compensate for the spacing deviations of objects entering a system comprised of three serially connected conveyors. The regulator acts to delay the time each object spends on the middle conveyor, eliminating spacing variations by the time objects exit the system. The system is experimentally tested to determine how effectively the algorithm can locate and deliver objects onto specific moving points. The limits of the regulator and the considerations for practical implementation are investigated. The proposed buffering regulator has immediate applications in the poultry processing industry, wherein live birds must be sorted and hung on a uniform shackle line moving at a constant speed.