Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-04T19:21:31Z No. of bitstreams: 1 KadignaCarlaSilvaCosta_DISSERT.pdf: 1273103 bytes, checksum: 42e60354cd1cf82d4ae965a4a92de036 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-12T23:33:41Z (GMT) No. of bitstreams: 1 KadignaCarlaSilvaCosta_DISSERT.pdf: 1273103 bytes, checksum: 42e60354cd1cf82d4ae965a4a92de036 (MD5) / Made available in DSpace on 2017-04-12T23:33:41Z (GMT). No. of bitstreams: 1 KadignaCarlaSilvaCosta_DISSERT.pdf: 1273103 bytes, checksum: 42e60354cd1cf82d4ae965a4a92de036 (MD5) Previous issue date: 2016-12-14 / Os morcegos atuam de diversas maneiras na regula??o dos ecossistemas. Dentre seus representantes, Artibeus planirostris ? uma esp?cie frug?vora, que atua tanto como dispersora de sementes quanto como polinizadora. Diante da escassez de estudos sobre a biologia reprodutiva de morcegos, sobretudo em machos, e da car?ncia de informa??es sobre a reprodu??o dos indiv?duos da ordem quir?ptera no Nordeste do Brasil, este estudo teve por objetivo quantificar o processo espermatog?nico de A. planirostris, bem como sua varia??o sazonal, atrav?s de an?lises morfol?gicas e morfom?tricas dos test?culos. Os animais foram coletados nos anos 2013 e 2014, entre as esta??es seca (n=10) e chuvosa (n=10), no campus central da Universidade Federal do Rio Grande do Norte (Natal-RN). Ap?s eutan?sia os test?culos foram coletados e processados histologicamente para inclus?o em historesina e analise sob microscopia de luz. As l?minas histol?gicas foram fotografadas e a morfometria foi realizada utilizando-se o software Image-Pro Plus. Os resultados obtidos quanto ? morfometria e biometria foram comparados pelo teste de Kruskall Wallis ou pelo teste de Student, considerando-se um n?vel de signific?ncia de 5% (p<0,05). Considerando-se as duas esta??es, os animais apresentaram ?ndice gonadossom?tico m?dio de 0,54%. Os t?bulos semin?feros representaram cerca de 92% do par?nquima testicular, sendo o restante representado pelo intert?bulo. Os t?bulos semin?feros foram compostos por cerca de 27% de l?men, 60% de epit?lio semin?fero e 4% de t?nica pr?pria. Dentre os par?metros morfom?tricos analisados, apenas o l?men apresentou varia??o significativa entre as esta??es, de modo que o maior percentual foi encontrado na esta??o chuvosa, com cerca de 29%. O di?metro tubular e a altura do epit?lio semin?fero apresentaram m?dias de 142 ?m e 43 ?m, respectivamente. Obteve-se comprimento tubular m?dio por grama de test?culo de 64,7 m e ?ndice tubulossom?tico de 0,47 %. A popula??o celular do epit?lio semin?fero mostrou-se composta por espermatog?nias, espermat?citos prim?rios na transi??o de pr?-lept?teno para lept?teno, em zig?teno e em paqu?teno, esperm?tides arredondadas e alongadas e c?lulas de Sertoli. Apenas a popula??o de espermat?citos prim?rios em pr?-lept?teno/lept?teno apresentou varia??o entre as esta??es, com maiores valores na esta??o seca. Obteve-se ?ndice mit?tico anual de 14%, ?ndice mei?tico de 3%, rendimento geral da espermatog?nese de 51 c?lulas e ?ndice de c?lulas de Sertoli de 6 c?lulas. A an?lise da frequ?ncia dos est?dios do ciclo do epit?lio semin?fero mostrou que os est?dios mais e menos frequentes foram, respectivamente, o 1 e o 6. Os est?dios 1 e 4 variaram estatisticamente entre as esta??es, sendo que o est?dio 1 foi mais frequente na esta??o chuvosa e o est?dio 4 foi mais frequente na esta??o seca. O intert?bulo foi composto predominantemente por c?lulas de Leydig, as quais apresentaram maiores percentuais no par?nquima testicular na esta??o seca, assim como a propor??o volum?trica dos vasos linf?ticos. Os demais componentes intertubulares n?o variaram estatisticamente entre as esta??es, bem como os par?metros analisados quanto ? morfometria das c?lulas de Leydig. Estas c?lulas apresentaram n?mero por grama de test?culo com m?dia anual de 5,63x107c?lulas, e ?ndice Leydigossom?tico de 0,005%. Observou-se grande investimento em t?bulos semin?feros, com baixa capacidade de suporte pelas c?lulas de Sertoli, e grande investimento em c?lulas de Leydig no par?nquima testicular, sobretudo na esta??o seca, bem como na popula??o de espermat?citos prim?rios em pr?-lept?teno/lept?teno indicando padr?o espermatog?nico anual cont?nuo no Nordeste do pa?s em A. planirostris. / Bats act in several ways to regulate ecosystems. Among its representatives, Artibeus planirostris is a frugivorous species, which acts both as a seed disperser and as a pollinator. Due to the scarcity of studies on the reproductive biology of bats, especially in males, and the lack of information about the reproduction of the specimens of order chiroptera in the Brazilian Northeast, this study aimed to quantify the spermatogenic process of A. planirostris, as well as its seasonal variation, through the morphological and morphometric analysis of the testes. The animals were collected in the years of 2013 and 2014, between the dry (n = 10) and rainy seasons (n = 10), at the central campus of the Federal University of Rio Grande do Norte (Natal-RN). After euthanasia, the testes were collected and processed histologically for embedding in historesin and analyzed under light microscopy. The histological slides were photographed and the morphometry was performed using the Image-Pro Plus software. The results of morphometry and biometry were compared by the Kruskall Wallis test or by the Student test, with a significance level of 5% (p <0.05). Considering the two seasons, the animals had a mean gonadosomatic index of 0,54%. The seminiferous tubules represented about 92% of the testicular parenchyma, the remaining being represented by the intertubule. The seminiferous tubules were composed of about 27% of lumen, 60% of seminiferous epithelium and 4% of tunica propria. Among the analyzed morphometric parameters, only the lumen showed significant variation between the seasons, so that the highest percentage was found in the rainy season, with about 29%. The tubular diameter and height of the seminiferous epithelium presented a mean of 142 ?m and 43 ?m, respectively. It was obtained a mean tubular length per gram of testis of 64.7 m and tubulesomatic index of 0.47%. The cell population of the seminiferous epithelium was composed of spermatogonia, primary spermatocytes in the transition from pre-leptotene to leptotene, zygotene and pachytene, rounded and elongated spermatids and Sertoli cells. Only the population of primary spermatocytes in pre-leptotene/leptotene presented variation between seasons, with higher values in the dry season. The annual mitotic index was 14%, the meiotic index was 3%, the overall spermatogenesis yield was 51 cells and the Sertoli cell index was 6 cells. The analysis of the frequency of the stages that compose the seminiferous epithelium cycle showed that the most and least frequent stages were, respectively, the 1 and the 6. Stages 1 and 4 varied statistically between seasons, with stage 1 being more frequent in the rainy season and stage 4 being more frequent in the dry season. The intertubule was predominantly composed of Leydig cells, which presented higher percentages in the testicular parenchyma in the dry season, as well as the volumetric proportion of the lymphatic vessels. The other intertubular components did not vary statistically between the seasons, as well as the parameters analyzed for Leydig cell morphometry. These cells presented a number per gram of testis with an annual mean of 5,63x107 cells and a Leydyssomatic index of 0.005%. It were observed a large investment in seminiferous tubules, with a low support capacity by Sertoli cells, and great investment in Leydig cells in the testicular parenchyma, especially in the dry season, as well as in the population of pre-leptotene/leptotene primary spermatocyte, indicating annual continuous spermatogenic pattern in the Northeast of the Brazil in A. planirostris.