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Analysis of gene expression in tumour immortalisationSeow, Choon Sheong January 2002 (has links)
Cellular senescence is an irreversible growth-arrested state seen in primary human cells after a finite number of cell divisions both in culture and <i>in vivo</i>. The escape from senescence to immortalisation is often thought to be a prerequisite for carcinogenesis. Many changes in senescent cells are consistent with changes in tissue and organ function with age. I used serial analysis of gene expression (SAGE) to analyse global gene expression profiles in senescent and early passage human foetal fibroblasts (hff). A total of over 20,000 SAGE tags were sequenced and characterised, corresponding to 2,675 unique transcripts. Relative to the early passage hff, transcripts which were found to be markedly increased in senescent hff included those encoding <i>p21<sup>WAF1</sup></i>, <i>Cyclin D1, </i>ferritin heavy chain, transforming growth factor-beta induced gene (<i>BIGH3</i>), skin collagenase and amyloid. Amongst them, genes such as skin collagenase and amyloid are known to be up-regulated in human ageing. Together with these known genes, a number of "unknown" genes were up-regulated. Seven differentially expressed genes, up-regulated in senescence (<i>BIGH3, </i>prion, dickkopf (Xenopus laevis) homolog 1 (<i>dkk1</i>), Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 (<i>CITED2</i>), <i>Rap1a</i> and Cysteine knot superfamily 1, bone morphogenetic protein antagonist 1 and retinoic acid receptor, alpha (<i>RARA</i>)) were selected for validation with reverse transcriptase-polymerase chain reaction (RT-PCR). The roles of these genes are discussed in relation to cancer and neurodegenerative diseases. My study demonstrates that replicative senescence is a complex phenomenon involving genes from the wingless-type (Wnt), mitogen-activated protein (Map) kinase kinases extracellular signal-regulated (Erk) kinase, transforming growth factor beta signalling and epigenetic pathways. The challenges remaining now are to identify senescence-related tumour suppressor gene(s) and to elucidate further the biochemical pathways of senescence.
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The role of sprouty2 in breast cancer tumorigenesis /Toher, Jessica L., January 2007 (has links) (PDF)
Thesis (M.S.) in Biochemistry--University of Maine, 2007. / Includes vita. Includes bibliographical references (leaves 50-54).
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Non-small cell lung cancer from bench to bedside /Ho, Chung-man. January 2007 (has links)
Thesis (M. D.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.
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The metabolism of benzo(a)pyrene in transformable and nontransformable C3H mouse fibroblast cell linesGehly, Eugene B. January 1981 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1981. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Interaction of polycyclic hydrocarbons with DNA of mouse skinGoshman, Lorna (McKinney), January 1967 (has links)
Thesis (Ph. D.)--University of Wisconsin, 1967. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Characterizing the dynamics of genome evolution in tumorigenesis /Woo, Yong, January 2009 (has links)
Thesis (Ph.D.) in Interdisciplinary--University of Maine, 2009. / Includes vita. Includes bibliographical references (leaves 140-170).
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The carcinogenicity and metabolism of several aromatic amines and their N̲-oxidized derivativesPoirier, Lionel A. January 1965 (has links)
Thesis (Ph. D.)--University of Wisconsin, 1965. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Physical and chemical effects of carcinogen binding to DNA in relation to biological activity and statistical problems in chemical carcinogenesisDrinkwater, Norman R. January 1980 (has links)
Thesis--University of Wisconsin--Madison. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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The role of prostaglandins in mouse skin tumor promotion and the tumor promoting phorbol ester receptor protein from its discovery to its role in the physiology of cell membranes /Ashendel, Curtis L. January 1982 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1982. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Factors contributing to the formation of CC-->TT tandem mutations /Skinner, Amy M. January 1900 (has links)
Thesis (Ph. D.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 134-145). Also available on the World Wide Web.
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