A microfluidic device was created for single-cell capture and analysis using polydimethylsiloxane (PDMS) channels and a glass substrate to develop a microfluidic single-cell impedance sensor for cell diagnostics. The device was fabricated using photolithography to create a master mold which in turn will use soft lithography to create the PDMS components for constant device production. The commercial software, COMSOLTM Multiphysics, was used to quantify the fluid dynamics in shallow micro-channels. The device will be able to capture a cell and sequester it long enough to enable measurement of the impedance spectra that can characterize cell. The proposed device will be designed to capture a single cell and permit back-flow to flush out excess cells in the chamber. The device will be designed to use syringe pumps and the syringe-controlled channel will also be used to capture and release the cell to ensure cell control and device reusability. We hypothesize that these characteristics along with other proposed design factors will result in a unique microfluidic cell-capture device that will enable single-cell impedance sensing and characterization.