This study determined whether a cadenced-based exercise prescription (CBEP) enabled participants to increase the time they spend doing moderate-to-vigorous intensity physical activity (MVPA) to a greater extent than Standard Care (SC). Forty-six participants were recruited from the ENCOURAGE study and were allocated to SC (n=25) or CBEP (n=21). Data was collected at baseline (T0), prior to the intervention (T1) and then again at 16 (T2) and 24 (T3) weeks. Moderate-to-vigorous physical activity was assessed by accelerometry in 10 minute (MVPA10min) and sporadic bouts (MVPASpor) as well as step counts (StepsTotal, StepsMVPA). Although participants reported that CBEP helped them to increase their motivation to become more active, none of the accelerometer parameters differed between SC and CBEP at baseline, 8, 16, or 24 weeks. These data suggest that CBEP did not have an added benefit for influencing physical activity over time amongst a population of previously sedentary adults.