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Thesis (M.S.)--Georgia State University, 2009. / Title from file title page. Jenny J. Yang, committee chair; Zhi-Ren Liu, Gangli Wang, Giovanni Gadda, committee members. Description based on contents viewed June 30, 2009. Includes bibliographical references (p. 116-119).

Thesis (M.S.)--Georgia State University, 2009. / Jenny J. Yang, committee chair; Zhi-Ren Liu, Gangli Wang, Giovanni Gadda, committee members. Typescript. Includes bibliographical references (leaves 101-104). Also available via the World Wide Web.

Thesis (Ph. D.)--Florida State University, 2005. / Advisor: Timothy S. Moerland, Florida State University, College of Arts and Sciences, Dept. of Biological Science. Title and description from dissertation home page (viewed May 11, 2006). Document formatted into pages; contains viii, 76 pages. Includes bibliographical references.

Cheung, Siu Yuen. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 178-200). / Abstracts in English and Chinese. / Abstract --- p.i / Chinese abstract --- p.iii / Acknowledgements --- p.v / Table of contents --- p.vii / Chapter Chapter One --- General Introduction --- p.1 / Chapter 1.1 --- S100 Proteins --- p.1 / Chapter 1.1.1 --- Structure of S100 proteins --- p.2 / Chapter 1.1.2 --- Possible functions of S100 proteins --- p.4 / Chapter 1.1.3 --- Genomic organization of S100 genes --- p.6 / Chapter 1.1.4 --- Clinical importance of S100 proteins --- p.7 / Chapter 1.2 --- S100A1 Protein --- p.8 / Chapter 1.2.1 --- Possible functions of the S100A1 protein --- p.10 / Chapter 1.2.1.1 --- Regulation of cardiac and skeletal muscle contractility --- p.10 / Chapter 1.2.1.2 --- Functional roles in the central nervous system (CNS) --- p.12 / Chapter 1.2.1.3 --- Other possible functions of the S100A1 protein --- p.13 / Chapter 1.2.2 --- S100A1 knockout mice --- p.14 / Chapter 1.2.3 --- Relationships between S100A1 and S100B proteins --- p.16 / Chapter 1.3 --- S100B Protein --- p.18 / Chapter 1.3.1 --- Possible functions of S100B protein --- p.19 / Chapter 1.3.2 --- S100B knockout mice --- p.20 / Chapter 1.4 --- RNA interference --- p.22 / Chapter 1.4.1 --- Mechanisms of RNA interference --- p.24 / Chapter 1.4.2 --- Efficacy and selectivity of siRNA --- p.25 / Chapter 1.4.3 --- siRNA delivery --- p.27 / Chapter 1.5 --- Objective --- p.31 / Figures and legends --- p.34 / Chapter Chapter Two --- S100A1 expression in normal mouse embryos and characterization of S100A1 knockout mouse embryos --- p.40 / Chapter 2.1 --- Introduction --- p.40 / Chapter 2.2 --- Materials and Methods --- p.44 / Chapter 2.2.1 --- Mouse strains --- p.44 / Chapter 2.2.2 --- RNA extraction --- p.46 / Chapter 2.2.3 --- Reverse Transcription-Polymerase Chain Reaction (RT-PCR) --- p.46 / Chapter 2.2.4 --- Protein extraction --- p.48 / Chapter 2.2.5 --- Western blotting --- p.49 / Chapter 2.2.6 --- Immunohistochemical staining --- p.50 / Chapter 2.3 --- Results --- p.53 / Chapter 2.3.1 --- S100A1 mRNA expression in normal mouse embryo --- p.53 / Chapter 2.3.2 --- S100A1 protein expression in normal mouse embryos --- p.55 / Chapter 2.3.2.1 --- Temporal expression of the S100A1 protein --- p.55 / Chapter 2.3.2.2 --- Spatial expression of the S100A1 protein --- p.57 / Chapter 2.3.3 --- Morphological and histological characterization of SI00A1 knockout mouse embryos --- p.60 / Chapter 2.3.4 --- S100B protein expression pattern in Wt and S100A1 KO mouse embryos --- p.62 / Chapter 2.4 --- Discussion --- p.64 / Tables --- p.73 / Figures and legends --- p.76 / Chapter Chapter Three --- Knockdown of S100A1 in S100B in knockout mouse embryos --- p.118 / Chapter 3.1 --- Introduction --- p.118 / Chapter 3.2 --- Materials and Methods --- p.128 / Chapter 3.2.1 --- Mouse strains --- p.128 / Chapter 3.2.2 --- Short-interfering RNA (siRNA) --- p.129 / Chapter 3.2.3 --- In-uterus surgery --- p.130 / Chapter 3.2.4 --- RNA extraction and RT-PCR --- p.132 / Chapter 3.2.5 --- Immunohistochemical staining of S100A1 and S100B --- p.132 / Chapter 3.3 --- Results --- p.133 / Chapter 3.3.1 --- Characterization of S100B knockout mouse embryos --- p.133 / Chapter 3.3.2 --- S100A1 knockdown in S100B wild-type (Wt) mouse embryos --- p.133 / Chapter 3.3.3 --- S100A1 knockdown in S100B knockout (KO) mouse embryos --- p.139 / Chapter 3.4 --- Discussion --- p.146 / Tables --- p.153 / Figures and legends --- p.154 / Chapter Chapter Four --- General Discussion and Conclusions --- p.175 / Reference --- p.178

Thesis (M.S.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains viii, 60 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 51-55).

An attempt to develop a short and reliable method of caldesmon purification led to the development of three procedures of caldesmon purification. The first method was seldom used because of its low yield and the lack of caldesmon endogenous kinase activity. However, it allowed us to purify MLCK (myosin light chain kinase). The second and third methods gave respectively, a caldesmon sample with and without kinase activity. We were able to localize the endogenous kinase in the 0-30% ammonium sulfate precipitated DEAE pellet but we were unsuccessful at purifying the kinase to homogeneity. We found that caldesmon can also be phosphorylated by rat brain Ca²⁺-calmodulin-dependent kinase II at sites identical to those of caldesmon endogenous kinase but different to those of kinase C. In addition, caldesmon and its endogenous kinase are two different proteins. Furthermore, our study of caldesmon inhibition of actomyosin ATPase activity showed that further research needs to be done to refute F-actin bundling process as a possible cause of caldesmon inhibition of actomyosin ATPase activity. In addition, our studies of caldesmon inhibition of HMM and S-1 ATPase activity suggest that S-2 might be partially involved in the inhibition mechanism. Finally, caldesmon did not affect the 6S-10S transition of myosin conformation and since caldesmon cannot compete against higher affinity calmodulin-binding protein such as MLCK thus, the flip-flop theory is untenable.

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