The loss of habitat to agriculture is a worldwide problem for biodiversity conservation. One species that has seemingly been able to adapt to the conversion of forests to farmlands is Mackinder’s eagle owl (Bubo capensis mackinderi), which inhabits highland areas, but little is known of its ecology, especially outside of protected areas. This study examined the impact of agricultural practices and farmer’s attitudes on the foraging and population ecology of the Mackinder’s eagle owl in central Kenya. Owl territories were monitored monthly from June 2004- October 2006 for signs of occupancy, breeding activity, mortality and to collect data on food resources. Nest site characteristics were measured for all known nests. Because previous studies showed an affinity for rodents, small mammals were trapped monthly using mark-recapture methodology. In each territory, the type and amount of farm crops were measured each month and farmers were interviewed about their knowledge and beliefs about owls. Mackinder’s eagle owls in central Kenya lived at extremely high density 0.87 owl pairs/km². This density was high compared to other populations of Mackinder’s eagle owl and to Eurasian eagle owl (Bubo bubo) populations in Europe. Breeding success was 48% over three years and this compared well with other species of eagle owl inhabiting human-disturbed areas. All nests and roosts were located in river valleys, and all successful nest sites were located on cliffs or other inaccessible rocky terrain. Nest sites were located adjacent to farms, which provided for both open hunting and an abundance of prey. Breeding activity was concentrated after the rainy seasons and this was likely linked to prey availability after the rains. Agricultural activities generally had a positive effect on rodent populations. Small mammal trapping results revealed that rodents were over 14 times more abundant in farms than in adjacent grassland habitat. This population of Mackinder’s eagle owl had a very catholic diet and consumed mostly mammalian prey species including hares, giant rats, root rats, grooved-tooth rats and small rodents. Small rodents accounted for almost half of the owls’ diet and when their numbers increased, owls responded by consuming more of them, indicating the importance of farming activities to this population of owls. Other populations of eagle owl inhabiting human-disturbed areas had diet widths positively related to levels of habitat disturbance. This result supported optimal foraging theory that more productive environments have predators with more specialized diets, while patchy environments have generalist predators. The ecology of this population of Mackinder’s eagle owls was heavily influenced by human agricultural activities, which generally had a positive effect on their population. Farming activities changed rapidly both within and between seasons as plots were small and neighbouring farmers planted various crops at different times of the year and this was enhanced by irrigation in some areas. Year-round availability of forage within farms had a positive effect on owl prey species, some of which increased relative to the type and amount of crops found in farms. However, 57% of owl injuries and mortalities that occurred were related either directly or indirectly to human activities. Cultural prejudices against owls remain the biggest threat to this population’s long-term persistence. Farmer education was shown to play a significant role in overcoming negative beliefs about owls. Because Mackinder’s eagle owls are highly adaptable to anthropomorphic landscape changes, largely due to their adaptability as food generalists, they are one of the few top predators remaining in this highly disturbed agricultural system. However, populations within agricultural areas remain especially vulnerable to negative human attitudes towards owls due to their close association with human activities.