published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy

Thesis (Ph. D.)--University of Hong Kong, 1995. / Includes bibliographical references.

published_or_final_version / Chemistry / Master / Master of Philosophy

Preparative-scale protein separations have always been critical to the advancement of the life sciences. Among preparative-scale separation techniques, isoelectric trapping (IET) promises efficient separations and high production rates. This dissertation focuses on the improvement of two aspects of preparative-scale IET protein separations: the instrumentation used and the monitoring of the separation. The first aspect (preparative-scale) is the IET device: the improvement of a multicompartmental electrolyzer (MCE) to increase the efficiency and production rate of IET separations. The redesign focused on three major areas: (1) the sealing system, (2) the configuration of the liquid flow path, and (3) the cooling system. The second aspect (analytical-scale) is the monitoring of the IET separation: the design and manufacture of durable surface-modified capillaries which provide controlled, variable anodic and cathodic electroosmotic flow (EOF) to help develop, plan, and monitor the IET separations.

Preparative-scale protein separations have always been critical to the advancement of the life sciences. Among preparative-scale separation techniques, isoelectric trapping (IET) promises efficient separations and high production rates. This dissertation focuses on the improvement of two aspects of preparative-scale IET protein separations: the instrumentation used and the monitoring of the separation. The first aspect (preparative-scale) is the IET device: the improvement of a multicompartmental electrolyzer (MCE) to increase the efficiency and production rate of IET separations. The redesign focused on three major areas: (1) the sealing system, (2) the configuration of the liquid flow path, and (3) the cooling system. The second aspect (analytical-scale) is the monitoring of the IET separation: the design and manufacture of durable surface-modified capillaries which provide controlled, variable anodic and cathodic electroosmotic flow (EOF) to help develop, plan, and monitor the IET separations.

Thesis (Ph. D.)--Florida State University, 2003. / Advisor: Dr. John G. Dorsey, Florida State University, College of Arts and Sciences, Dept. of Chemistry and Biochemistry. Title and description from dissertation home page (viewed Sept. 08, 2004). Includes bibliographical references.

Thesis (M. Phil.)--University of Hong Kong, 1996. / Year of submission on cover: 1996. Includes bibliographical references (leaf 87-89).

Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references (leaves 123-126). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references (leaves 123-126). Also available on the Internet.

Thesis (M.S.)--Brock University, 2006. / Includes bibliographical references (leaves 83-112). Also available online (PDF file) by a subscription to the set or by purchasing the individual file.