Khaled, Maha Yehia.
Thesis (Ph. D.)--Virginia Polytechnic Institute and State University, 1994. Vita. Abstract. Includes bibliographical references (leaves 222-229). Also available via the Internet.
Salimi-Moosavi, Mir Mohammad Hossein
1 January 1997
The use of nonaqueous solvents in capillary electrophoresis (CE) was investigated. Nonaqueous solvents used included methanol, ethanol, acetonitrile, dimethylformamide, and molten salts. Both indirect, and direct UV detection as well as electrochemical detection were examined. The behavior of electroosmotic flow and Joule heating was examined as well as selectivity patterns for a variety of inorganic and organic ionic species. Analytes examined included inorganic anions, alkali and alkaline earth metal ions, alkanesulfonates, alkyl sulfates, linear alkylbenzensulfonates, derivatized and free amino acids, and fatty acids. Selectivity was dependent not only on the nature of the solvent, but also on the nature and concentration of the electrolyte. With many inorganic ions completely reversed separation order relative to aqueous systems was observed. Although the addition of metal ions caused changes in resolution, complete separation of alkanesulfonates (C2-C16) and alkyl sulfates (C8-C18) was possible by a change from protic (methanol) to aprotic conditions (addition of acetonitrile). The partial separation of positional isomers of linear alkyl benzensulfonate homologues (C10-C15) was achieved in methanol/acetonitrile mixtures. The complete separation of alkali and alkaline earth metal ions, which has not been reported to date, and separation of potassium and ammonium were observed in a methanol/imidazole electrolyte. Significantly different migration order was achieved for dansylated amino acids compared to aqueous systems. Changes in selectivity of free amino acids were possible in basic and acidic electrolytes. Complete separation of a wide range of fatty acids (C1-C20) was possible in less than 20 min. The unique selectivity patterns in nonaqueous solvents was related to changes in solvation and ion-interaction effects. The ability to easily adjust selectivity via choice of electrolyte and to inject aqueous samples directly into the nonaqueous electrolytes suggests that such systems may offer significant advantages for many analytical problems. The results illustrate that when selectivity, efficiency, quantitation, and detection limits are taken into consideration, nonaqueous CE approaches should, in many situations, offer a unique alternative to aqueous based CE analyses. Electroosmotic flow was appreciable, and was cathodal in basic solutions. Reversal of electroosmotic flow was observed in the presence of an excess of a strong acid (HCl or HClO4) in the separation electrolyte. The nature of electrolyte anion (chloride, perchlorate, and acetate) and solvent (methanol/acetonitrile mixtures) had significant effects on the electroosmotic flow in acidic conditions. Evidence was observed for ion adsorption (protons and anions) on to silica surfaces and ion-interaction in the electrolyte. Adsorption and ion-interaction could be used to control both the direction and magnitude of electroosmotic flow. Reproducibility of electroosmotic flow was good under appropriate experimental conditions (%RSD = 1.1).
Determination of capillary electrophoretic methodology : its application as a mechanistic tool for gaining insight into the composition of, and detergent action on, coloured macromolecule fabric stainsPretswell, Emma Louise 1995 (has links)
No description available.
Baynham, Michael Thomas
No description available.
Thesis (Ph.D.)--York University, 2005. Graduate Programme in Chemistry. Typescript. Includes bibliographical references (leaves 140-150). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pNR11550
Buica, Astrid Sorina.
Thesis (Ph. D.)--University of Stellenbosch, 2007. Includes bibliographical references. Also available via the Internet.
Separation of proteins with capillary electrophoresis in coated capillaries with and without electroosmosis : studies on zone broadening and analytical performancesMohabbati, Sheila 2006 (has links)
Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. Härtill 4 uppsatser.
A rapid method for detecting single nucleotide polymorphisms using antimicrobial resistance in Neisseria gonorrhoeae as a modelCullingham, Kyle 26 April 2005 (has links)
Chromosomal mediated antimicrobial resistance in Neisseria gonorrhoeae can develop as a result of three main processes including the alteration of target enzymes, changes in transmembrane transport channels and active efflux pump function. Single nucleotide polymorphisms (SNPs) of target genes such as DNA gyrase (gyrA) and topoisomerase (parC), together with mutations in the promoter regions of the efflux pumps norM and mtr can confer resistance to the macrolides, penicillins and fluoroquinolones. These SNPs were analyzed using the SNaPshot method to allow for rapid detection of resistant isolates. Oligonucleotides were developed in the 5’ to the 3’ direction, ending one nucleotide adjacent to the specific SNP of interest. Single base extension reactions were performed and were detected using capillary electrophoresis. The SNaPshot procedure from Applied Biosystems employed in this study adds a single fluorescently-labelled nucleotide complementary to this SNP at the 3’ end by a primer extension polymerase reaction. Then using capillary electrophoresis, the labelled nucleotide is detected, enabling differentiation between A, C, T, or G. SNP results obtained were verified using DNA sequencing and both single and multiplexed reactions were carried out to increase the efficiency of the procedure. Spiked urine samples were also observed to determine if SNPs could be detected clinically. Single reactions enabled the characterization of all confirmed and relevant SNPs. With multiplex primer extension, multiple peaks were observed, each corresponding to one of the SNPs in the gene. This technique was explored for its applicability to detect SNPs of gyrA and parC mutations. Observable SNP detection limits were seen in spiked urine samples at 108 cells/mL in as early as 4 hours. DNA sequencing results confirmed the SNPs identity in each case. Thus, capillary electrophoresis using the SNaPshot protocol is another way to rapidly identify clinically resistant strains of Neisseria gonorrhoeae. This technique has also been shown to reduce analysis time compared to DNA sequencing and produces the same results. February 2005
Chan, Yick Chuen.
Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2002. Includes bibliographical references (leaves 78-80). Also available in electronic version. Access restricted to campus users.
Development and validation of analytical methodologies for the determination of ionic compounds in environmental, biological and industrial samples by capillary zone electrophoresisLau, Kap-man. 1997 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1998. Includes bibliographical references (leaves 402-404).
Page generated in 0.0644 seconds