Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T13:35:08Z No. of bitstreams: 2 thalita_gomes_miranda.pdf: 848359 bytes, checksum: ea292a0faa794975c2a67fb133b57b6a (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T17:58:54Z (GMT) No. of bitstreams: 2 thalita_gomes_miranda.pdf: 848359 bytes, checksum: ea292a0faa794975c2a67fb133b57b6a (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2016-01-05T17:58:57Z (GMT). No. of bitstreams: 2 thalita_gomes_miranda.pdf: 848359 bytes, checksum: ea292a0faa794975c2a67fb133b57b6a (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2014 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / As pimentas (g?nero Capsicum spp.) s?o mundialmente conhecidas e apreciadas como condimentos al?m de serem excelentes fontes de nutrientes. A crescente demanda faz com que aumente a busca por cultivares que atendam ?s necessidades do mercado para consumo in natura e para fins industriais. A caracteriza??o f?sico-qu?mica dos gen?tipos de pimenta serve de base para o melhoramento gen?tico e lan?amentos de novas cultivares no mercado. Diante disso, o presente trabalho teve como objetivo avaliar gen?tipos de pimenta Capsicum chinense e C. annuum, quanto ?s caracter?sticas f?sico-qu?micas. Foram realizados dois experimentos, um para avalia??es f?sicas e qu?micas e outro para avaliar o teor de capsaicina presente nos frutos. Os experimentos foram conduzidos em casa-de-vegeta??o, em DBC com oito gen?tipos e quatro repeti??es, sendo os gen?tipos BGH-433, BGH-4285, PIM-030, Numex Sweet, Numex Garnet e os h?bridos F1 (Numex Sweet x PIM-030), F1 (Numex Garnet x PIM-030), F1 (BGH-4285 x BGH-433). Para o primeiro experimento foram avaliadas as seguintes caracter?sticas: produtividade total de frutos, peso m?dio do fruto, n?mero de sementes por fruto, altura da planta, produtividade de mat?ria seca total, porcentagem de mat?ria seca do fruto, porcentagem de mat?ria seca da polpa, porcentagem de mat?ria seca da semente, espessura do pericarpo, comprimento do fruto, fen?licos totais, flavonoides, pH, s?lidos sol?veis, vitamina C e acidez titul?vel. No segundo experimento foi avaliado o conte?do de capsaicina presente na semente, na polpa e no fruto inteiro e determinada a pung?ncia dos gen?tipos. Os h?bridos F1 (Numex Sweet x PIM-030), F1 (Numex Garnet x PIM-030) e F1 (BGH-4285 x BGH-433) e as linhagens Numex Garnet e Numex Sweet apresentaram alta produtividade total de frutos e de mat?ria seca total de frutos. O h?brido F1 (BGH-4285 x BGH-433) apresentou altos valores de heterose e heterobeltiose para produtividade total de frutos e produtividade de mat?ria seca total de frutos, e apresentou heterose e heterobeltiose positivos para a porcentagem de mat?ria seca da semente, j? o h?brido F1 (Numex Sweet x PIM-030) apresentou heterose e heterobeltiose negativas para essa caracter?stica. O h?brido F1 (BGH-4285 x BGH-433) apresentou heterobeltiose negativa para a porcentagem de mat?ria seca do fruto. H? correla??es positivas entre caracter?sticas de interesse que podem contribuir significativamente com os programas de melhoramento com C. chinense e C. annuum por meio da sele??o indireta. Os valores observados para os compostos fen?licos, flavonoides, pH, s?lidos sol?veis, vitamina C e acidez total n?o variaram significativamente entre os gen?tipos estudados. O gen?tipo BGH-433 apresentou as maiores concentra??es de capsaicina para semente e polpa. Os h?bridos F1 (BGH-4285 x BGH-433) e BGH-433 apresentaram as maiores concentra??es de capsaicina para fruto inteiro. Os gen?tipos BGH-433, F1 (BGH-4285 x BGH-433) e BGH-4285 foram classificados pela SHU como moderadamente pungentes. Os gen?tipos Numex Sweet, Numex Garnet, PIM-030, F1 (Numex Garnet x PIM-030) e F1 (Numex Sweet x PIM-030) foram classificados como n?o pungentes a levemente pungentes. A temperatura no per?odo de condu??o do experimento pode ter influenciado negativamente na pung?ncia dos frutos, pois alguns gen?tipos apresentaram pung?ncia menor que o esperado. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. / ABSTRACT Peppers (Capsicum spp.) are world known and appreciated as condiments and are excellent sources of nutrients. The increasing demand causes increase of the search for cultivars that meet the needs of the market for fresh consumption and for industrial purposes. The physicochemical characterization of genotypes of pepper is the basis for the breeding and release of new cultivars on the market. Therefore, this study aimed to evaluate genotypes of pepper Capsicum chinense and C. annuum, as the physical and chemical characteristics. Two experiments, one for physical and chemical tests and another to evaluate the content of capsaicin present in the fruits were performed. The experiments were conducted in a greenhouse house in DBC with eight genotypes and four replications, with the genotypes BGH-433, BGH-4285, PIM-030, Numex Sweet, Numex Garnet and F1 hybrids (Numex Sweet x PIM-030), F1 (Numex Garnet x PIM-030), F1 (BGH-4285 x BGH-433). For the first experiment the following characteristics were evaluated: total fruits yield, average fruit weight, number of seeds per fruit, plant height, total dry matter yield, dry matter content of the fruit dry matter content of the pulp, percentage of dry matter of seed, pericarp thickness, fruit length, total phenolics, flavonoids, pH, soluble solids, vitamin C and titratable acidity. In the second experiment evaluated the content of capsaicin present in the seed, in the pulp and whole fruit and given the poignancy of genotypes. The F1 hybrids (Numex Sweet x PIM-030), F1 (Numex Garnet x PIM-030) and F1 (BGH-4285 x BGH-433) and the lines Numex Garnet and Numex Sweet had high total fruit yield and dry matter total fruit. The F1 hybrid (BGH-4285 x BGH-433) showed high levels of heterosis and heterobeltiosis for total fruits yield and productivity of total dry fruits, and showed positive heterosis and heterobeltiosis for dry matter content in the seed, since the hybrid F1 (Numex Sweet x PIM-030) show heterosis and negative heterobeltiosis for this trait. The F1 hybrid (BGH BGH x-4285-433) showed negative for the percentage of dry matter of the fruit heterobeltiosis. There are positive correlations between features of interest that can contribute significantly to the improvement programs with C. chinense and C. annuum by indirect selection. The observed values of the phenolic compounds, flavonoids, pH, soluble solids, total acidity and vitamin C did not differ significantly between genotypes. The BGH-433 genotype showed the highest concentrations of capsaicin for seed and pulp. The F1 hybrids (BGH BGH x-4285-433) and BGH-433 showed the highest concentrations of capsaicin for whole fruit. The BGH-433, F1 genotypes (BGH-4285 x BGH-433) and BGH-4285 were classified as moderately pungent by SHU. Genotypes Numex Sweet, Numex Garnet, PIM-030, F1 (Numex Garnet x PIM-030) and F1 (Numex Sweet x PIM-030) were classified as non-pungent and slightly pungent. The temperature in driving time of the experiment may have negatively influenced the pungency of the fruits, because some genotypes showed less pungency than expected.

Made available in DSpace on 2014-06-11T19:34:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-12-15Bitstream added on 2014-06-13T19:24:03Z : No. of bitstreams: 1 marubayashi_jm_dr_botfca.pdf: 931972 bytes, checksum: 9c460e6d355ca8d5a17b125ad750bfb2 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Tomato severe rugose virus – ToSRV é um vírus pertencente ao gênero Begomovirus da família Geminiviridae, proveniemte de pimentão e transmitido pelo aleirodídeo Bemisia tabaci biótipo B. Este biótipo B foi introduzido no início dos anos 90, é um inseto polífago reproduzindo-se em mais de 500 espécies de plantas anuais e herbáceas. Causa danos diretos como a sucção de seiva com ação toxicogênica e aparecimento de fumagina, e danos indiretos pela transmissão de vírus, principalmente os begomovírus. O presente trabalho teve como objetivo estudar a interação do isolado ToSRV [PJU] com o vetor Bemisia tabaci biótipo B, avaliar a atratividade do inseto à diversos acessos de Capsicum spp., e determinar os biótipos de mosca-branca encontrados no Estado de São Paulo. Para avaliar a eficiência de transmissão do vírus pelo inseto foram realizadas as combinações tomateiro para tomateiro (T/T), tomateiro para pimentão (T/P), pimentão para pimentão (P/P) e pimentão para tomateiro (P/T). As melhores condições de transmissão foram observadas com temperaturas ao redor de 30 ºC, a partir de T/T e P/T. Quando diferentes números de insetos foram utilizados houve um aumento na transmissão, exceto para a combinação de P/P, onde não foi verificada esta correlação. Com relação ao período de acesso à aquisição, foi observado que maiores tempos de aquisição promoveram aumento na transmissão do vírus pela mosca-branca para T/T e P/T, enquanto que na combinação T/P e P/P, menores tempos de aquisição permitiram uma melhor transmissão.Utilizando-se um período de acesso à inoculação mínima de 15 minutos, foi possível a transmissão do vírus pelo inseto, exceto na combinação P/T e quanto maior este período, maior a taxa de transmissão. Não foi possível avaliar o período de latência, utilizando-se apenas um inseto e transferindo-o durante... / Tomato severe rugose virus - ToSRV is a virus belonging to the genus Begomovirus, family Geminiviridae, isolated from sweetpepper and transmitted by the aleyrodideo Bemisia tabaci biotype B. This new biotype B was introduced in the beginning of years 90 and it is an insect that multiplies in more than 500 species of annual and herbaceous plants. It toxicogenic action causes damages by suction the plants, the development of a fungus, fumagina, and these insects are vectors of different species of viruses, mainly begomovirus. The objective of this work, was to evaluate the interaction of the isolate ToSRV[PJU] with the vector Bemisia tabaci biotype B, to evaluate the attractiveness of the insect for the diverse genotypes of Capsicum spp, and to determine the biotype of whitefly in the State of São Paulo. To evaluate the efficiency of transmission of the virus by the insect different combinations were analyzed: tomato for tomato (T/T), tomato for sweetpepper (T/SP), sweetpepper for sweetpepper (SP/SP) and sweetpepper for tomato (SP/T). The best conditions of transmission were observed with temperatures around of 30 °C, from T/T and SP/T. Generally higher numbers of insects increased the transmission of the virus, but in the combination of SP/SP this was not observed. The acquisition access period was analysed and demonstrated that bigger times increased the transmission of the virus by the whitefly in the combinations T/T and SP/T. This was not observed in combination T/SP and SP/SP. The minimum period access of inoculation was of 15 minutes, except in combination SP/T. With one insect it wasn´t possible to evaluate the period of latency of the virus. It was verified that the leaves of the apex and intermediary of 38 different Capsicum spp. genotypes are the most attractive places for the whitefly and have the h highest egg concentration. The most attractive access was Capsicum frutescens... (Complete abstract click electronic access below)

Resumo: Tomato severe rugose virus - ToSRV é um vírus pertencente ao gênero Begomovirus da família Geminiviridae, proveniemte de pimentão e transmitido pelo aleirodídeo Bemisia tabaci biótipo B. Este biótipo B foi introduzido no início dos anos 90, é um inseto polífago reproduzindo-se em mais de 500 espécies de plantas anuais e herbáceas. Causa danos diretos como a sucção de seiva com ação toxicogênica e aparecimento de fumagina, e danos indiretos pela transmissão de vírus, principalmente os begomovírus. O presente trabalho teve como objetivo estudar a interação do isolado ToSRV [PJU] com o vetor Bemisia tabaci biótipo B, avaliar a atratividade do inseto à diversos acessos de Capsicum spp., e determinar os biótipos de mosca-branca encontrados no Estado de São Paulo. Para avaliar a eficiência de transmissão do vírus pelo inseto foram realizadas as combinações tomateiro para tomateiro (T/T), tomateiro para pimentão (T/P), pimentão para pimentão (P/P) e pimentão para tomateiro (P/T). As melhores condições de transmissão foram observadas com temperaturas ao redor de 30 ºC, a partir de T/T e P/T. Quando diferentes números de insetos foram utilizados houve um aumento na transmissão, exceto para a combinação de P/P, onde não foi verificada esta correlação. Com relação ao período de acesso à aquisição, foi observado que maiores tempos de aquisição promoveram aumento na transmissão do vírus pela mosca-branca para T/T e P/T, enquanto que na combinação T/P e P/P, menores tempos de aquisição permitiram uma melhor transmissão.Utilizando-se um período de acesso à inoculação mínima de 15 minutos, foi possível a transmissão do vírus pelo inseto, exceto na combinação P/T e quanto maior este período, maior a taxa de transmissão. Não foi possível avaliar o período de latência, utilizando-se apenas um inseto e transferindo-o durante... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Tomato severe rugose virus - ToSRV is a virus belonging to the genus Begomovirus, family Geminiviridae, isolated from sweetpepper and transmitted by the aleyrodideo Bemisia tabaci biotype B. This new biotype B was introduced in the beginning of years 90 and it is an insect that multiplies in more than 500 species of annual and herbaceous plants. It toxicogenic action causes damages by suction the plants, the development of a fungus, fumagina, and these insects are vectors of different species of viruses, mainly begomovirus. The objective of this work, was to evaluate the interaction of the isolate ToSRV[PJU] with the vector Bemisia tabaci biotype B, to evaluate the attractiveness of the insect for the diverse genotypes of Capsicum spp, and to determine the biotype of whitefly in the State of São Paulo. To evaluate the efficiency of transmission of the virus by the insect different combinations were analyzed: tomato for tomato (T/T), tomato for sweetpepper (T/SP), sweetpepper for sweetpepper (SP/SP) and sweetpepper for tomato (SP/T). The best conditions of transmission were observed with temperatures around of 30 °C, from T/T and SP/T. Generally higher numbers of insects increased the transmission of the virus, but in the combination of SP/SP this was not observed. The acquisition access period was analysed and demonstrated that bigger times increased the transmission of the virus by the whitefly in the combinations T/T and SP/T. This was not observed in combination T/SP and SP/SP. The minimum period access of inoculation was of 15 minutes, except in combination SP/T. With one insect it wasn't possible to evaluate the period of latency of the virus. It was verified that the leaves of the apex and intermediary of 38 different Capsicum spp. genotypes are the most attractive places for the whitefly and have the h highest egg concentration. The most attractive access was Capsicum frutescens... (Complete abstract click electronic access below) / Orientador: Marcelo Agenor Pavan / Coorientador: Renate Krause Sakate / Banca: Antonio Carlos Maringoni / Banca: Edson Luiz Lopes Baldin / Banca: Valdir Atsushi Yuki / Banca: Romulo Fujito Kobori / Doutor

Las enfermedades de etiología viral destacan por ser el principal factor limitante de las hortalizas. La estrategia más eficaz para el control consiste en la identificación de genes de resistencia y el posterior desarrollo de variedades resistentes. El principal objetivo de esta tesis se centra en el aprovechamiento y la caracterización de la variabilidad genética de las especies cultivadas del género Capsicum (C. annuum, C. chinense, C. frutescens, C. baccatum y C. pubescens). Se ha puesto a punto una plataforma de EcoTILLING que permite un cribado eficiente de la colección de entradas de Capsicum del Banco de Germoplasma del COMAV. Esta plataforma se exploró para la búsqueda de variantes alélicas de los genes eIF4E y eIF(iso)4E, implicados en la resistencia a virosis. Una colección de 31 entradas que representan distintas combinaciones de los alelos eIF4E y eIF(iso)4E se inocularon mecánicamente con el Potato virus Y (PVY) y el Tobacco etch virus (TEV). Cinco nuevas variantes del gen eIF4E (pvr210, pvr211, pvr212, pvr213, pvr214) están relacionadas con la respuesta de resistencia al PVY y al TEV. Diez microsatélites y cuatro combinaciones de AFLPs se utilizaron para caracterizar 260 entradas del género Capsicum. Se detectó la existencia de cierta estructuración intraespecífica relacionada geográficamente. C. baccatum y C. pubescens muestran un par de grupos genéticos, por un lado están las entradas procedentes de Bolivia y por el otro las entradas de Ecuador y Perú. Además, las entradas de C. chinense procedentes de Perú pudieron ser diferenciadas del resto. En este estudio las entradas de Ecuador y Perú han mostrado una diversidad elevada y similar a las de Bolivia, el cual es un importante centro de diversidad del género Capsicum. Por tanto, las entradas procedentes de estos países podrán constituir fuentes importantes de variación para ser utilizadas en los programas de mejora del pimiento. / Ibiza Gimeno, VP. (2011). Nuevas herramientas en la lucha contra las virosis del pimiento [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/11549 / Palancia

Five separate field experiments were conducted across different environmental locations in Texas for the purpose of quantifying concentrations of different phytochemical groups (ascorbic acid, capsaicinoids, and flavonoids) within various pepper species, as well as, to identify the most optimum environment to promote expression of the aforementioned phytochemical. Depending on the particular experiment, quantitative measurements were then used in more detail in one of three ways: for identification of the most superior individuals and optimum environmental locations to express elevated concentrations of a particular phytochemical (first three experiments), to calculate heritability and % heterosis estimates for various fruit characteristics and phytochemical levels (fourth experiment), or for use in a specific biotechnology technique to potentially identify a molecular marker linked to elevated levels of ascorbic acid (AA) and flavonoids (quercetin and luteolin) (fifth experiment). In the first experiment, significant differences in fruit weight, capsaicin, and dihydrocapsaicin (DHC) were revealed in fruit tissue of five Habanero (Capsicum chinense) hybrids in comparison to a popular, commercial check (Kukulkan F1) after being grown across three different environmental locations (College Station, Uvalde, and Weslaco). Fruit grown at the Weslaco location was found to be larger and contained more capsaicin and DHC than those produced in Uvalde or College Station. While flavonoid contents were variable and low in all genotypes and locations, a few hybrids showed some potential for use in future crossing schemes to compete against the commercial check. Our results further suggested that variation in phytochemicals in fruit tissue of Habanero genotypes can be exploited by selecting in an appropriate environment. In the second experiment, analysis of four jalapeño hybrids in comparison to three commercial checks (Dragon, Ixtapa, and J1845), as well as, two cayenne hybrids in comparison to one commercial check (Mesilla) were compared after growing in three different locations (Amarillo, College Station, and Uvalde). Results demonstrated that the College Station location contributed to production of fruit containing higher concentrations of both AA and flavonoids, while those grown in Amarillo produced fruit with higher capsaicinoids. As expected, cayenne samples contained more AA and flavonoids than jalapeño samples. In comparison to the commercial jalapeño checks, all jalapeño hybrids generally expressed less capsaicinoids. With respect to AA and flavonoid concentrations, a few jalapeño hybrids proved to have some potential to compete against their respective commercial cultivars. For the cayenne market, one genotype revealed its potential use in mild markets and for farmers interested in a new hybrid expressing both appreciable levels of phytochemicals, as well as, more visually aesthetic attributes. Statistical analyses from the third experiment found all the F-values for each characteristic to be significant except the Location x Genotype (L x G) component for fruit wall thickness. For this experiment, a total of 21 different C. annuum (jalapeño, Serrano, and cayenne) genotypes were evaluated after growing in two diverse environmental locations (Uvalde and Weslaco). In general, peppers grown in Weslaco produced fruit with higher concentrations of AA and capsaicinoids, with a few exceptions, while fruit grown at Uvalde generally was larger in size and contained higher concentrations of flavonoids. In comparison to their respective commercial checks (jalapeño – Dragon, Tormenta; Serrano – Halcon, Magnum45; cayenne – Mesilla), a few hybrids were arguably more desirable for their respective markets with respect to different fruit measurements, AA, capsaicinoid, and flavonoid concentrations. This evidence further suggested the potential benefit this material could have for growers interested in replacing current material in the industry and to more successfully provide consumers with a healthier ingredient to better sustain their lives. In the fourth experiment, results confirmed our hypotheses that paprika type material has higher AA and flavonoid concentrations than Serrano peppers, while the opposite is possible for capsaicinoid expression. For this experiment, our goal was to identify the relative ease of incorporating different traits of interest into an improved specimen evaluating a combination of 29 F1 paprika and Serrano pepper (C. annuum) hybrids along with 19 of their respective parents. We were also able to identify how productive certain crossing combinations were at expressing a significant amount of positive heterosis for different characteristics of interest. From our correlation analyses, we were able to identify that a significant association can exist between fruit weight and fruit diameter, as well as when other characteristics were evaluated. In all, our results were able to reveal how effective certain combinations of parent material are towards production of offspring with improved traits expressing appreciable fruit characteristics and elevated phytochemical concentrations. Finally, the quantitative measurements produced in our F2 molecular marker experiment found significant amounts of variation for both flavonoids (quercetin and luteolin) and AA expression. It was evident that incorporation of the necessary genes of interest present within fruit tissue across this F2 family for these different phytochemicals were passed from ‘Ca377’ (P1) to several of these offspring. Three candidate primers were eventually identified for their potential polymorphic expression. However, after statistically analyzing the results, only one primer was identified as expressing a significant association (although the value was still relatively low) of variability with respect to luteolin. We also identified a significant association between quercetin and luteolin, quercetin and total flavonoids (quercetin+luteolin), as well as, luteolin and total flavonoids (quercetin+luteolin) in fruit tissues of different pepper material. From our results, we were able to arguably conclude that an environmental component may serve a more essential role in activating the necessary physiological processes to produce specific secondary metabolites. Although our RAPD technique was fairly straightforward and useful, some may argue that a more reliable technique would have been better. Therefore, we postulate that success may be possible in the near future if a different molecular marker technique is used.

Submitted by Maria Beatriz Vieira (mbeatriz.vieira@gmail.com) on 2017-03-29T13:15:52Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_vania_marques_gehling.pdf: 823705 bytes, checksum: bb9a04c4074928df8353da917cbc35a4 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-05T17:44:43Z (GMT) No. of bitstreams: 2 dissertacao_vania_marques_gehling.pdf: 823705 bytes, checksum: bb9a04c4074928df8353da917cbc35a4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-05T17:45:44Z (GMT) No. of bitstreams: 2 dissertacao_vania_marques_gehling.pdf: 823705 bytes, checksum: bb9a04c4074928df8353da917cbc35a4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-04-05T17:45:54Z (GMT). No. of bitstreams: 2 dissertacao_vania_marques_gehling.pdf: 823705 bytes, checksum: bb9a04c4074928df8353da917cbc35a4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-09-03 / Sem bolsa / O objetivo deste trabalho foi avaliar o desempenho fisiológico de sementes e plântulas de pimenta e de tomateiro sob influência de diferentes temperaturas. Foram conduzidos, separadamente, dois experimentos no Laboratório Didático de Análise de Sementes – FAEM - UFPel. No primeiro experimento foram utilizadas sementes de pimenta Cayenne Long Slim®, e no segundo experimento foram empregadas sementes de tomateiro Gaúcho®. Em ambos os experimentos, os tratamentos consistiram de temperaturas constantes, sendo: 18; 20; 22; 24 e 26ºC. Para a determinação do desempenho fisiológico das sementes e plântulas foram empregadas as avaliações de germinação, primeira contagem de germinação, índice de velocidade de germinação, envelhecimento acelerado, teste de frio, comprimento de parte aérea e de raiz primária, massa da matéria seca de parte aérea e de raízes, emergência de plântulas aos 14 e 21 dias após a semeadura e índice de velocidade de emergência. Os resultados demonstraram que a germinação de sementes de pimenta não é afetada de maneira marcante pelas temperaturas de 18 a 24oC, assim como sementes de tomateiro sob temperatura de 18 a 26oC, que não apresentaram diferença significativa na germinação. A primeira contagem de germinação, o índice de velocidade de germinação e os valores de envelhecimento acelerado foram incrementados linearmente com o aumento da temperatura, em pimenta e tomateiro. Todavia, o teste de frio e de emergência, tanto aos 14 como aos 21 dias após a semeadura, foram influenciados positivamente, até a temperatura aproximada de 22ºC, para ambas as culturas. Deste modo, a expressão do vigor de sementes, de ambas as espécies, é afetada negativamente pela redução da temperatura de desenvolvimento, sendo resultados marcantes obtidos nas temperaturas de 18 e 20oC. / The objective of this study was to evaluate the physiological performance of seeds and pepper and tomato seedlings under the influence of different temperatures. They were conducted separately in two experiments in the Didactic Laboratory of Seed Analysis - FAEM - UFPel. In the first experiment pepper seeds were used Cayenne Long Slim®, and in the second experiment Gaúcho® tomato seeds were employed. In both experiments, the treatments consisted of constant temperatures, as follows: 18; 20; 22; 24 and 26ºC. To determine the physiological performance of the seeds and seedlings were employed germination assessments, first count, germination speed index, accelerated aging, cold test, shoot length and primary root mass of dry matter air and root, seedling emergence at 14 and 21 days after sowing and emergence speed index. The results show that the pepper seed germination is not affected markedly by the temperature from 18 to 24ºC, as well as tomato seeds at a temperature of 18 to 26ºC, which showed no significant difference in germination. The first count, germination speed index and the accelerated aging values were increased linearly with increasing temperature in pepper and tomato. However, the cold test and emergency, both at 14 and at 21 days after sowing, were influenced positively to the approximate temperature of 22°C, for both crops. Thus, the expression of seed vigor of both species is adversely affected by reducing the growth temperature, with remarkable results obtained at temperatures of 18 to 20ºC.