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The aim of the diploma thesis was to evaluate the content of soil organic carbon in the area of Novohradsko. Soil organic carbon was monitored in its stable (SOC) and labile (WSOC) form, followed by the total organic carbon stock (Cpool). These soil parameters were determined in soils of different land cover types (forest, arable land, grassland) in the area formed by the catchments of Pasecký, Bedřichovský, Váčkový and Veverský stream. Data obtained in 2001, 2007 and 2014 were statistically analysed and the comparison of the data from different catchments was conducted. The results show the impact of different land use on the amount of soil organic carbon. In general, a higher amount of soil organic carbon were found in soils of grassland and forest soils, whereas a lower amounts were found in arable land. The comparison and the time development analysis show that there is a trend of the increase in quantity of stable fraction of soil organic carbon in all categories of land cover over the whole monitored period. The opposite trend of a continuous decrease over the whole of the monitored period in all categories of land cover was observed in amounts of labile fraction of soil organic carbon. It can be said that there is an increase in the stock of a stable fraction of soil organic carbon due to lower losses of the labile fraction of soil organic carbon in forest soils, arable soils and soils of grassland.