This study estimated monthly and annual Net Primary Productivity (NPP), an important indicator of carbon sequestration, in the Conterminous US from 1997 to 2007 using Carnegie-Ames-Stanford Approach. Vegetation condition, temperature, precipitation, photosynthetically active radiation and soil water holding capacity were used as model’s inputs. NPP values were lower than mean annual values during the year 2000 to 2003 which was probably due to extreme drought conditions during these years. Higher NPP per square meter was generally found in Savannah and Subtropical eco-divisions whereas Tropical/Subtropical deserts had the lowest NPP. Southeastern states had the highest NPP per square meter thus, made the highest contribution to the terrestrial carbon sequestration in US. Since the vegetation is one of the main factors in NPP and thus carbon sequestration, the results of this study could help in various environmental policy decisions on forest and cropland management at the state, EPA and federal levels.