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Thesis (M.S.)--University of Michigan, 1986.

Thesis (M.S.)--West Virginia University, 2005. / Title from document title page. Document formatted into pages; contains x, 114 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 69-77).

Articular cartilage is an avascular, aneural tissue that covers the ends of diarthroidal joints. Once damaged by disease or injury, cartilage lacks the ability to self-repair. Generating tissue engineered cartilage is an exciting field that may provide a possible solution to this problem. The purpose of this study is to determine the efficacy of a through-thickness perfusion bioreactor to generate scaffoldree tissue engineered cartilage. The results of the study show that allowing long-term static culture to cell constructs before perfusion increases the efficacy of the bioreactor. Immediate perfusion of cell constructs in the bioreactor is shown to decrease the efficacy to produce scaffoldree constructs with desirable biomechanical and biochemical properties. The results of the study also show possible options in future works that could increase the efficacy of the bioreactor.

Thesis (Ph. D.)--University of Missouri--Columbia, 2004. / Typescript. Vita. Includes bibliographical references. Also issued on the Internet.

Thesis (doctoral)--Karolinska Institutet, Stockholm, 1989. / Extra t.p. with thesis statement inserted. Includes bibliographical references.

Thesis (Ph. D.)--University of Missouri--Columbia, 2004. / "July 2004." Typescript. Vita. Includes bibliographical references. Also issued on the Internet.

Thesis (doctoral)--Karolinska Institutet, Stockholm, 1989. / Extra t.p. with thesis statement inserted. Includes bibliographical references.

by Linda, Fu Lap Kun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 115-128). / ACKNOWLEDGMENT --- p.i / TABLE OF CONTENTS --- p.ii / ABSTRACT --- p.xii / Chapter CHAPTER ONE: --- INTRODUCTION --- p.1 / Chapter CHAPTER TWO : --- LITERATURE REVIEW --- p.6 / Chapter 2.1 --- BIOLOGY OF ARTICULAR CARTILAGE --- p.7 / Chapter 2.1.1 --- Normal cartilage --- p.7 / Chapter 2.1.1.1 --- Composition and properties --- p.7 / Chapter 2.1.1.2 --- Morphology of normal cartilage --- p.12 / Chapter 2.1.2 --- Degeneration of cartilage --- p.12 / Chapter 2.1.2.1 --- Morphology of degenerative cartilage --- p.12 / Chapter 2.1.2.2 --- Biochemical characteristics of degenerative cartilage --- p.13 / Chapter 2.1.3 --- Healing of degenerated articular cartilage --- p.14 / Chapter 2.2 --- METHODS TO CREATE OSTEOARTHRITIS MODEL --- p.15 / Chapter 2.2.1 --- Immobilization --- p.16 / Chapter 2.2.2 --- Instability method --- p.16 / Chapter 2.3 --- SCORING OF OSTEOARTHRITIS --- p.17 / Chapter 2.3.1 --- Gross morphology --- p.17 / Chapter 2.3.1.1 --- Mapping of OA area --- p.17 / Chapter 2.3.1.2 --- Amount of synovial fluid --- p.18 / Chapter 2.3.1.3 --- Mobility --- p.18 / Chapter 2.3.2 --- Histological scoring --- p.18 / Chapter 2.3.3 --- Biochemical comparison --- p.21 / Chapter 2.3.3.1 --- Water content --- p.21 / Chapter 2.3.3.2 --- Proteoglycan --- p.21 / Chapter 2.4 --- THE TREATMENT OF OSTEOARTHRITIS --- p.23 / Chapter 2.4.1 --- Biology of Hyaluronic acid --- p.24 / Chapter 2.4.2 --- Repairing properties of Hyaluronic acid in OA --- p.24 / Chapter 2.4.3 --- The pharmacology of Hyalgan --- p.26 / Chapter 2.4.4 --- Intraarticular injection of Hyaluronic acid in animals --- p.26 / Chapter CHAPTER THREE: --- CREATING OSTEOARTHRITIC MODEL --- p.28 / Chapter 3.1 --- INTRODUCTION --- p.29 / Chapter 3.2 --- MATERIAL AND METHODOLOGY --- p.30 / Chapter 3.2.1 --- Animals --- p.30 / Chapter 3.2.2 --- Instabilization --- p.32 / Chapter 3.2.2.1 --- Reagent and Apparatus --- p.32 / Chapter 3.2.2.2 --- Procedure --- p.32 / Chapter 3.2.3 --- Immobilization --- p.36 / Chapter 3.2.3.1 --- Reagent and Apparatus --- p.36 / Chapter 3.2.3.2 --- Procedure --- p.37 / Chapter 3.3 --- METHODS OF ASSESSMENT --- p.39 / Chapter 3.3.1 --- Gross structure comparison --- p.40 / Chapter 3.3.1.1 --- Gross mapping of the OA areas --- p.40 / Chapter 3.3.2 --- Histochemical scoring --- p.40 / Chapter 3.3.2.1 --- Procedure --- p.40 / Chapter 3.3.2.1.1 --- Hematoxylin & eosin stain --- p.45 / Chapter 3.3.2.1.2 --- Safranin O stain --- p.45 / Chapter 3.3.3 --- Biochemical assessment --- p.47 / Chapter 3.3.3.1 --- Water content --- p.47 / Chapter 3.3.3.2 --- Proteoglycan content --- p.49 / Chapter 3.3.3.2.1 --- Reagent --- p.49 / Chapter 3.3.3.2.2 --- Procedure --- p.49 / Chapter 3.3.3.2.3 --- Standard curve --- p.52 / Chapter 3.4 --- METHODS OF STATISTICAL ANALYSIS --- p.53 / Chapter 3.5 --- RESULTS --- p.54 / Chapter 3.5.1 --- Gross morphology --- p.54 / Chapter 3.5.1.1 --- General appearance --- p.54 / Chapter 3.5.1.2 --- Gross osteoarthritic area --- p.56 / Chapter 3.5.2 --- Histological results --- p.59 / Chapter 3.5.2.1 --- Histological appearance --- p.59 / Chapter 3.5.3 --- Results of biochemical assay --- p.66 / Chapter 3.3.3.1 --- Water content --- p.66 / Chapter 3.5.3.2 --- Proteoglycan --- p.69 / Chapter 3.6 --- DISCUSSION --- p.72 / Chapter 3.6.1 --- Relation between gross OA area & Mankin's score --- p.72 / Chapter 3.6.2 --- Relation between gross OA area & GAG content --- p.74 / Chapter 3.6.3 --- Relation between Mankin's score & water content --- p.76 / Chapter 3.6.4 --- Relation between Mankin's score & GAG content --- p.78 / Chapter 3.6.5 --- Comparison of the four different parameters --- p.79 / Chapter 3.6.6 --- Merit on the four parameters --- p.84 / Chapter 3.6.6 --- Consistency & reproducible of the OA model --- p.85 / Chapter 3.6.7 --- Moderately severe OA model was needed in Part II --- p.86 / Chapter 3.7 --- CONCLUSION --- p.86 / Chapter CHAPTER FOUR: --- HEALING OF CARTILAGE --- p.87 / Chapter 4.1 --- INTRODUCTION --- p.88 / Chapter 4.2 --- MATERIALS AND METHODS --- p.89 / Chapter 4.2.1 --- Establishing the osteoarthritis --- p.89 / Chapter 4.2.2 --- Treatment of the rabbits --- p.90 / Chapter 4.2.2.1 --- Reagent and Apparatus --- p.90 / Chapter 4.2.2.2 --- Procedure --- p.91 / Chapter 4.2.3 --- Methods of assessment --- p.92 / Chapter 4.2.4 --- Methods of statistical analysis --- p.92 / Chapter 4.3 --- RESULTS --- p.93 / Chapter 4.3.1 --- Gross OA area --- p.94 / Chapter 4.3.2 --- Mankin's score --- p.97 / Chapter 4.3.3 --- Water content --- p.99 / Chapter 4.3.4 --- Proteoglycan . . --- p.101 / Chapter 4.3.5 --- Results of Part I and Part II --- p.103 / Chapter 4.4 --- DISCUSSION --- p.107 / Chapter 4.4.1 --- Morphological comparison --- p.107 / Chapter 4.4.2 --- Biochemical comparison --- p.110 / Chapter 4.4.3 --- Comparison of the results in two parts of experiment --- p.111 / Chapter 4.5 --- CONCLUSION --- p.113 / BIBLIOGRAPHY --- p.114 / APPENDIX --- p.129

Thesis (Ph.D.)--University of Alberta, 2010. / A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Experimental Surgery, Department of Surgery. Title from pdf file main screen (viewed on February 17, 2010). Includes bibliographical references.