Thesis (M.S.)--University of Wisconsin--Madison, 1968. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

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Fifteen adenoviruses from cattle, sheep, horses, and llamas were studied by virus neutralization to determine their degree of antigenic similarity. Prototype viruses included bovine adenoviruses species 1-8, ovine adenoviruses species 5 and 6, and equine adenovirus species 1. Unclassified viruses that were compared to the prototype viruses were isolated from different locations within Oregon and were represented by bovine isolate 32CN, ovine isolates 47F and 475N, and llama isolate 7649. Reciprocal virus neutralization tests were performed and the degree of antigenic similarity, i.e., species differentiation was determined by criteria established by the International Committee for the Nomenclature of Viruses. The study showed that many of the adenoviruses, both prototype and unclassified, shared minor antigenic components with each other. Prototype viruses possessed major antigenic differences and, as previously demonstrated by other investigators, should be classified as separate virus species. Bovine adenovirus isolate 32CN was shown to be of the same species as ovine adenovirus isolate 475N, but neither isolate was similar to any of the prototype virus species studied. Ovine adenovirus isolate 47F was shown to be of the same species as ovine adenovirus species 5 strain RTS 42. Llama adenovirus isolate 7649, while sharing minor antigens with different viruses from cattle and sheep, was shown to be a distinct species. This represents the first species of adenovirus from llama. / Graduation date: 1993

An enzyme-linked immunosorbent assay was developed to measure specific antibody response in bovine sera to bovine adenovirus type 3 (BA3), an etiologic agent of respiratory disease causing economic losses annualy to the cattle industry. Observed endpoint titers were determined using the intersection point from optical density values of serially diluted sera with a positive-negative threshold. Regression equations were determined from standards with titers ranging from low to high and used to predict ELISA titers from a single-serum dilution. A near-linear relationship existed between the observed and predicted ELISA titers of 118 bovine sera (r=0.9261). Predicted ELISA titers were determined using the single-dilution method for another 76 bovine sera and the correlation between the ELISA titers and serum-virus neutralization titers for these sera indicated a strong linear trend (r=0.8172). Both the ELISA and serum-virus neutralization titers on the bovine sera tested indicated widespread exposure to several types of bovine adenovirus. Although detection of active infection would still require examination of sera over time for evidence of a rising titer, the single-dilution ELISA devised should provide a rapid and sensitive method for detection of antibody response to bovine adenovirus type 3. / Graduation date: 1992