Grace Chung Tin Yun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 129-146). / SUMMARY / INTRODUCTION --- p.1 / LITERATURE REVIEW / Chapter 2.1 --- Anatomy and Histology of Uterine Cervix --- p.4 / Chapter 2.2 --- Cervical carcinoma --- p.5 / Chapter A. --- Incidence / Chapter B. --- Pathology and histological classification / Chapter C. --- Etiological factors / Chapter 2.3 --- Human Papillomavirus (HPV) --- p.12 / Chapter 2.4 --- Human papillomavirus and cervical carcinoma --- p.15 / Chapter 2.5 --- Principle of Polymerase Chain Reaction (PCR) --- p.17 / Chapter A. --- DNA structure / Chapter B. --- Repetitive DNA synthesis by PCR / Chapter 2.6 --- Basis of Restriction Fragment Length Polymorphism analysis (RFLP) --- p.18 / Chapter 2.7 --- RFLP analysis and cancer --- p.19 / Chapter 2.8 --- Oncogenes and Suppressor genes --- p.21 / Chapter A. --- Oncogenes / Chapter B. --- Tumour Suppressor Genes / Chapter 2.9 --- Multistep process of carcinogenesis --- p.24 / Chapter 2.10 --- Oncogenes and Suppressor genes in cervical carcinoma --- p.25 / Chapter A. --- Oncogenes / Chapter B. --- Tumour Suppressor Genes / MATERIALS AND METHODS / Chapter 3.1 --- Specimens --- p.41 / Chapter A. --- Cervical scrapes / Chapter B. --- Cervical tissues / Chapter C. --- Blood / Chapter 3.2 --- Extraction of Genomic DNA --- p.42 / Chapter A. --- Cervical scrapes / Chapter B. --- Tissue samples / Chapter C. --- Blood / Chapter 3.3 --- Southern blot --- p.45 / Chapter 3.4 --- Polymerase Chain Reaction (PGR) --- p.47 / Chapter 3.5 --- Nucleic Acid Probes --- p.49 / Chapter A. --- Oligonucleotide probes / Chapter B. --- Human papillomavirus / Chapter C. --- Chromosome-specific RFLP/VNTR probes / Chapter D. --- H-ras and c-myc proto-oncogenes / Chapter 3.6 --- Radioactive Labelling of Probes --- p.51 / Chapter A. --- Oligonucleotide probes / Chapter B. --- Nucleic Acid probes / Chapter C. --- Removal of unincorporated label / Chapter 3.7 --- Nucleic Acid Hybridization --- p.54 / RESULTS / Chapter 4.1 --- Optimize MgCl2 concentration for Polymerase chain reaction --- p.72 / Chapter 4.2 --- Sensitivity of Polymerase chain reaction --- p.72 / Chapter 4.3 --- Detection of HPV in cervical scrapes by PCR --- p.73 / Chapter 4.4 --- Detection of HPV in cervical scrapes using PCR with each primer-set --- p.73 / Chapter 4.5 --- Detection of HPV in cervical tissues using PCR --- p.73 / Chapter 4.6 --- Detection of HPV in cervical tissues by Southern blot hybridization --- p.74 / Chapter 4.7 --- Analysis of cervical tissues with chromosome 3-specific probes --- p.75 / Chapter 4.8 --- Analysis of cervical tissues with proto-oncogene probes H-ras and c-myc --- p.77 / Chapter 4.9 --- Analysis of cervical tissues with chromosome 17-specific probes mapped to 17pl3 --- p.77 / DISCUSSION / Chapter 5.1 --- Evaluation of methods --- p.111 / Chapter 5.2 --- Analysis of HPV using PCR --- p.114 / Chapter 5.3 --- PCR vs Southern blot hybridization in HPV detection --- p.117 / Chapter 5.4 --- HPV DNA status in cervical neoplasia and cervical cancer --- p.118 / Chapter 5.5 --- Genetic lesions in cervical neoplasia and cervical cancer --- p.119 / Chapter 5.6 --- HPV infection and genetic lesions in cervical cancer --- p.125 / CONCLUSION --- p.127 / REFERENCE --- p.129