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Caracterização química e enzimática do processo de adoçamento da manga \'Keitt\' / Chemical and enzymatic characterization of the mango Keitt sweetening processAna Paula Fioravante Bernardes Silva 16 June 2004 (has links)
Dentre as características que definem um fruto maduro, o adoçamento é um dos mais importantes. Porém, no que concerne à manga, os dados existentes são escassos e pouco esclarecedores. Neste trabalho foi estudada a síntese e a degradação do amido da manga \'Keitt\', nos aspectos químicos (teores de amido, de amilose e de açúcares solúveis), nos aspectos bioquímicos (atividade de enzimas relacionadas à degradação do amido, perfil de enzimas ligadas ao grânulo de amido) e aspecto morfológico do grânulo de amido (preliminar), e o amadurecimento do fruto. A manga \'Keitt\' teve um padrão atípico de fruto climatérico, com a produção de pequenas quantidades de etileno e C02, culminando em picos após o processo de amadurecimento ter sido iniciado. Todo o amido acumulado (cerca de 8 %) durante o desenvolvimento até os 3 dias após a colheita (dpc), foi totalmente degradado a partir dos 5 dpc chegando ao final do amadurecimento com apenas traços do seu conteúdo inicial. Ao mesmo tempo acumulou cerca de 10 % de açúcares solúveis, com predominância da sacarose. As enzimas que potencialmente podem degradar o amido, tiveram perfil de atividade compatível com a sua atuação. Houve um aumento bastante significativo de atividade da α-amilase durante a formação do fruto e da β--amilase durante o amadurecimento do fruto. As fosforilases e isoamilases, embora tivessem atividade suficiente para atuar durante a degradação do amido, demonstraram pelo perfil de atividade terem bastante importância durante a síntese do amido. Os grânulos de amido, como observados por microscopia eletrônica de varredura, tem grânulos redondos, lisos e pequenos (até 20 µm), que diminuem de tamanho durante o amadurecimento da manga. As proteínas ligadas ao grânulo, como visto por eletroforese em condições dissociantes, aumentaram em número e quantidade depois da colheita da manga, mostrando que várias proteínas de alto e baixo peso molecular, aderiram ao grânulo antes do início da degradação. / Sweetening is one of the most important characteristics concerning ripe fruit. However, physiological and biochemical changes associated to mango fruit ripening process, including mango sweetening, is still poor understood. In this work the synthesis and breakdown of the starch were studied focusing starch, amylose, and soluble sugars content. Also the activities of some enzymes that participate in starch metabolism were evaluated during development and ripening of mango \'Keitt\'. Thought Scanning Electron Microscopy, granule starch shape and superficial changes related to the degree of mango ripening, were investigated. Results shown that mango fruit (cv. Keitt) has not a typical profile of ethylene and respiration during the ripening process, with very low leveis of both parameters. There was a massive conversion of the starch accumulated (~8 % )during fruit development to soluble sugar (~10 %), with a predominance of sucrose. The activity of α-amylase increased at least 20 times during fruit development while β:-amylase showed detectable activity after fruit harvesting. Starchphosphorylases and isoamylases activities can be linked with both starch synthesis and degradation. Granule starch isolated from mango, showed spherical shape and small size (~20 µm) when the mango achieve full starch content (~3 days after harvest), with about 25 % of amylose. During fruit ripening, granule size suffered superficial corrosion and decreased in size (~6 µm). SDS-PAGE gels showed that after harvesting increased the number of starch bounded proteins with low and high molecular weight, inclusive inside the granule. These proteins can be associated with starch degradation process.
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Ammonia threshold and ammonia measurement as potential physiological parameters. / Ammonia threshold and ammonia measurement as potential physiologic parameters / CUHK electronic theses & dissertations collection / Digital dissertation consortiumJanuary 2002 (has links)
"May 2002." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (p. 142-163). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. Ann Arbor, MI : ProQuest Information and Learning Company, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.
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Biothermodynamic studies of blood components with special reference to biocompatibilityLjunggren, Lennart. January 1994 (has links)
Thesis (doctoral)--Lund University, 1994. / Added t.p. with thesis statement inserted.
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Het zinkgehalte van het bloed bij normale en zieke personenWeiland, Johan Paul Ernst Sperna. January 1946 (has links)
Proefschrift--Utrecht. / Summaries in Dutch and English. "Literatuur": p. [82]-92.
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Studier over den Flammefotometriske Metodes Fejlkilder og Anvendelighed til Bestemmelse af Kalium og Natrium i Blod og Urin. [Studies on the sources of error and service ability of the flame-photometric method for determination of the potassium and sodium contents of blood and urine].Ryssing, Erik. January 1952 (has links)
Afhandling - Copenhagen. / English summary. "Litteraturfortegnelse": p. 229-[240].
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Biothermodynamic studies of blood components with special reference to biocompatibilityLjunggren, Lennart. January 1994 (has links)
Thesis (doctoral)--Lund University, 1994. / Added t.p. with thesis statement inserted.
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Estudo do tratamento quimico da solu‡ao de fluoreto de amonio proveniente da unidade de reconversao de uranioFRAJNDLICH, ELITA U. de C. 09 October 2014 (has links)
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Avaliacao do metodo de dosagem de pregnandiol urinario por cromatografia a gasACHANDO, SETSUKO S. 09 October 2014 (has links)
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Estudo comparativo do veneno de Bothrops jararaca do continente e de especimes da Ilha de Sao Sebastiao / Comparative study of Bothrops jararaca venom from mainland and specimens of the island of São SebastiãoRIBEIRO JUNIOR, MARCOS A. 09 October 2014 (has links)
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Contribuicao ao estudo das consequencias quimicas da reacao (n, gamma) nos compostos n-benzoil n-fenil hidroxilaminato de cobre e n-benzoil N-(o) toluil hidroxilaminato de cobreNAKANISHI, CASUE 09 October 2014 (has links)
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