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This diploma thesis is focused on the influence of the three most represented capsaicinoids – capsaicin, dihydrocapsaicin and nordihydrocapsaicin – on the sensory properties of five varieties of chili peppers. Aji Lemon Drop, Serrano, Jamaican Yellow, Habanero Chocolate and Bhut Jolokia were selected for this thesis. Determination of capsaicinoids content was performed using HPLC-DAD. The content of capsaicin in dried fruits ranged from 359 to 320 gg-1, the content of dihydrocapsaicin in the range of 130–7 767 gg-1 and the content of nordihydrocapsaicin in the range of 7–456 gg-1. The pungency of the samples was 8 928–601 338 SHU. The sensory evaluation of selected varieties of chili peppers was attended by 20 evaluators representing ordinary consumers. The subject of the evaluation was mainly the intensity of burning and sharpness, its pleasantness and identification of the place of manifestation of thermal sensations. Furthermore, the onset of burning and sharpness from the insertion of peppers into the mouth, the duration of thermal sensations, the intensity of sweetness and juiciness, the presence of other flavors and the texture of the fruit. Statistically significant differences were observed between the evaluation results. The low intensity of burning and sharpness of the Jamaican Yellow variety was assessed as neutral to rather pleasant, while the high intensity of thermal sensations of the Habanero Chocolate and Bhut Jolokia varieties was perceived as unpleasant. The Aji Lemon Drop and Serrano varieties were judged very similar from a sensory point of view. By combining the results of capsaicinoids content measurement and sensory analysis, significant correlation relationships were found between the evaluated parameters. With a higher content of capsaicinoids in peppers, their pungency (expressed in SHU) increased, at the same time a higher intensity of thermal sensations and their longer duration were found during consumption. On the contrary, with a high intensity of burning and sharpness, the pleasantness of these perceptions decreased. The onset of these feelings did not affect the observed properties. The higher the intensity of juiciness, the higher the sweetness of the fruit; however, these properties were not significant in terms of the content of combustibles or the intensity of thermal sensations. The effect of individual capsaicinoids on the site of thermal sensation has not been statistically proven.