Ho Hoi Yan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 107-118). / Abstracts in English and Chinese. / Abstract --- p.i / Abstract (Chinese) --- p.iii / Acknowledgments --- p.v / Contents --- p.vii / List of Tables --- p.x / List of Figures --- p.xi / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter Chapter 2 --- Literature Review --- p.5 / Chapter 2.1 --- Introduction to phylogenetic biology --- p.5 / Chapter 2.1.1 --- Definition of phylogenetics --- p.5 / Chapter 2.1.2 --- Why employ molecular genetic markers in phylogenetics? --- p.5 / Chapter 2.2 --- DNA analysis and the contributions to phylogenetics --- p.7 / Chapter 2.2.1 --- Nuclear ribosomal DNA --- p.7 / Chapter 2.2.2 --- Animal mitochondrial DNA (mt DNA) --- p.10 / Chapter 2.3 --- Molecular phylogeny of crustaceans --- p.11 / Chapter 2.3.1 --- Phylogenetic studies of crustaceans using nuclear rRNA --- p.11 / Chapter 2.3.2 --- Population genetic and phylogenetic studies of crustaceans using mtDNA --- p.14 / Chapter 2.4 --- Taxonomy of the genus Eriocheir --- p.23 / Chapter 2.5 --- Distribution of Eriocheir s.l --- p.27 / Chapter 2.6 --- Population genetics of Eriocheir --- p.28 / Chapter 2.6.1 --- Population genetics of Eriocheir japonica --- p.28 / Chapter 2.6.2 --- Population genetics and history of invasion of Eriocheir sinensis --- p.29 / Chapter Chapter 3 --- Materials and Methods --- p.32 / Chapter 3.1 --- Specimens --- p.32 / Chapter 3.1.1 --- Specimens for phylogenetic reconstruction --- p.32 / Chapter 3.1.2 --- Specimens for population genetic study on E. sinensis --- p.33 / Chapter 3.2 --- DNA extraction --- p.33 / Chapter Chapter 3 --- Materials and Methods --- p.32 / Chapter 3.1 --- Specimens --- p.32 / Chapter 3.1.1 --- Specimens for phylogenetic reconstruction --- p.32 / Chapter 3.1.2 --- Specimens for population genetic study on E. sinensis --- p.33 / Chapter 3.2 --- DNA extraction --- p.33 / Chapter 3.3 --- Amplification of genes --- p.36 / Chapter 3.3.1 --- PCR profile --- p.38 / Chapter 3.3.1.1 --- 16S rRNA gene --- p.38 / Chapter 3.3.1.2 --- COI gene --- p.40 / Chapter 3.3.1.3 --- ITS-1 gene --- p.41 / Chapter 3.4 --- DNA sequencing --- p.41 / Chapter 3.4.1 --- Purification of extension products --- p.43 / Chapter 3.4.2 --- Electrophoresis and data collection --- p.44 / Chapter 3.4.3 --- Sequence alignment --- p.44 / Chapter 3.5 --- Phylogenetic construction --- p.45 / Chapter Chapter 4 --- Results --- p.47 / Chapter 4.1 --- Phylogenetic reconstruction of Eriocheir --- p.47 / Chapter 4.1.1 --- PCR products of mitochondrial 16S rRNA and COI gene and nuclear ribosomal ITS-1 gene --- p.47 / Chapter 4.1.2 --- Intraspecific variation --- p.49 / Chapter 4.1.3 --- Genetic variability in mitten crabs based on partial sequences of 16S rRNA gene --- p.49 / Chapter 4.1.4 --- Genetic variability in mitten crabs based on partial sequences of COI gene --- p.53 / Chapter 4.1.5 --- Genetic variability in mitten crabs based on complete sequences of ITS-1 gene --- p.62 / Chapter 4.1.6 --- Phylogenetic analysis --- p.66 / Chapter 4.1.6.1 --- Phylogenetic analysis based on 16S rDNA sequences --- p.66 / Chapter 4.1.6.2 --- Phylogenetic analysis based on COI gene sequences --- p.70 / Chapter 4.1.6.3 --- Phylogenetic analysis based on ITS-1 gene sequences --- p.73 / Chapter 4.2 --- Population genetic study of E. sinensis --- p.77 / Chapter 4.2.1 --- PCR products and intraspecific variation of mitochondrial COI gene for population genetic study of E. sinensis --- p.77 / Chapter 4.2.2 --- Genetic variability in E. sinensis based on partial sequences of COI gene --- p.79 / Chapter 4.2.3 --- Population genetic analysis based on COI gene sequences --- p.86 / Chapter Chapter 5 --- Discussion --- p.93 / Chapter 5.1 --- Phylogeny of Eriocheir --- p.93 / Chapter 5.1.1 --- Phylogenetic relationships --- p.93 / Chapter 5.1.2 --- Taxonomic implications --- p.95 / Chapter 5.1.3 --- Evolutionary history of Eriocheir --- p.97 / Chapter 5.2 --- Population study of E. sinensis --- p.101 / Chapter 5.2.1 --- Genetic variation between Chinese populations --- p.101 / Chapter 5.2.2 --- Genetic variation between native and introduced populations --- p.102 / Chapter Chapter 6 --- Conclusions --- p.104 / Literature Cited --- p.107