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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
181

THE METABOLISM OF CHROMATIN-ASSOCIATED PROTEINS IN VICIA FABA AND THEIR RESPONSES TO GROWTH REGULATORS

Grahek, Barbara Suzanne Hauser, 1944- January 1973 (has links)
No description available.
182

IN VITRO EFFECT OF TESTOSTERONE, PROGESTERONE, AND 17-BETA ESTRADIOL ON THE OCCURRENCE OF "Y" BODY AND BARR BODY IN INTERPHASE NUCLEI OF MAN

Samsam-Bakhtiary, Sianoosh, 1946- January 1975 (has links)
No description available.
183

Systematic analysis of the interactome of modified chromatin

Nikolov, Miroslav 19 October 2012 (has links)
No description available.
184

Promyelocytic Leukemia Nuclear Bodies: A Meeting Place For Genomic Loci

Ching, Reagan Wai Kit 15 November 2013 (has links)
The nucleus is a highly compartmentalized organelle where specific cellular activities are confined to discrete domains. One such domain is the promyelocytic leukemia nuclear body (PML NB). PML NBs are protein-based structures that make numerous contacts with neighboring chromatin domains. To elucidate the function of PML NBs, research has been focused on identifying the protein complement of PML NBs. More than 60 proteins have been shown to localize to PML NBs, implicating the bodies in numerous cellular activities such as transcription regulation, apoptosis, tumor suppression, and the antiviral response. This approach has not yielded a general model for PML NB function. Instead I have chosen to focus on the chromatin contacts made with PML NBs. Using live-cell microscopy, my observations support the hypothesis that changes in chromatin topology affect the structural integrity of PML NBs. Moreover, I have developed a technique, called immunoTRAP, which allows for the extraction of chromatin specifically associated with PML NBs. Analysis of these chromatin associations reveal that specific genes associate with PML NBs and these associations are cell type specific. Therefore, PML NBs make specific contacts with neighboring chromatin domains and these contacts are integral to PML NB morphology. Thus making PML NBs a meeting place for a specific set of genomic loci.
185

Promyelocytic Leukemia Nuclear Bodies: A Meeting Place For Genomic Loci

Ching, Reagan Wai Kit 15 November 2013 (has links)
The nucleus is a highly compartmentalized organelle where specific cellular activities are confined to discrete domains. One such domain is the promyelocytic leukemia nuclear body (PML NB). PML NBs are protein-based structures that make numerous contacts with neighboring chromatin domains. To elucidate the function of PML NBs, research has been focused on identifying the protein complement of PML NBs. More than 60 proteins have been shown to localize to PML NBs, implicating the bodies in numerous cellular activities such as transcription regulation, apoptosis, tumor suppression, and the antiviral response. This approach has not yielded a general model for PML NB function. Instead I have chosen to focus on the chromatin contacts made with PML NBs. Using live-cell microscopy, my observations support the hypothesis that changes in chromatin topology affect the structural integrity of PML NBs. Moreover, I have developed a technique, called immunoTRAP, which allows for the extraction of chromatin specifically associated with PML NBs. Analysis of these chromatin associations reveal that specific genes associate with PML NBs and these associations are cell type specific. Therefore, PML NBs make specific contacts with neighboring chromatin domains and these contacts are integral to PML NB morphology. Thus making PML NBs a meeting place for a specific set of genomic loci.
186

Characterizing the interactions between mouse nucleoplasmin and chromosomal proteins

Ellard, Katherine 20 December 2012 (has links)
The family of Nucleoplasmin (NPM) proteins play an important role in a number of chromatin remodelling processes. The first NPM protein discovered in the eggs and oocytes of Xenopus laevis was NPM2, a tissue specific histone chaperone. In Xenopus, NPM2 has been linked to paternal chromatin decondensation following fertilization through the removal of sperm proteins, nucleosome assembly through the storage and addition of H2A-H2B dimers and apoptosis. In mammals, NPM2 correlates strongly with nucleolus-like bodies, and has been suggested by various groups to differ in its roles when compared to the X. laevis homologue. However, the exact roles of NPM2 in mammals remain to be fully elucidated. In this dissertation, attempts are made to determine the physical interaction sites between mouse NPM2 and core histone proteins, H2A, H2B, H3 and H4, as well as physical interactions between mouse NPM2 and protamines (sperm proteins) P1 and P2. Interaction sites between mouse NPM2 and various chromosomal proteins were investigated using a number of different techniques. First, NPM2: chromosomal protein binding assays were attempted to determine the ratio of NPM2 to both core histones and protamines. When visualized through 12% Native gels, NPM2 was determined to interact with histone octamers at a molar ratio of 1-1.5 mol NPM2/mol histone octamer. Mouse sperm protamines were determined to form complexes with mouse NPM2 at a molar ratio of 2.5 mol protamine/mol NPM2 (or mol protamine/0.4 mol NPM2). Analytical Ultracentrifuge (AUC) analysis was conducted on NPM2 and chromosomal proteins separately and in complex formation. Although determining that isolated, full length mouse NPM2 exists in a pentamer form, attempts with AUC were unsuccessful in determining specific NPM2:chromosomal protein binding affinity and complex formation. Specific physical interaction sites between NPM2 and chromosomal proteins were investigated using Cross Linking Mass Spectrometry. Here, a number of new interaction sites as well as sites previously identified by other groups were determined. In combination, our results present likely interaction sites between NPM2 and chromosomal proteins and represent an interesting point of reference for future work. / Graduate
187

Expression of stem-loop binding protein during murine oogenesis and pre-implantation development

Champigny, Marc. January 1998 (has links)
The goal of the work presented here was to investigate the hypothesis that cytoplasmic SLBP is required for translation of somatic H1 mRNA, and their translational repression is due to a lack of SLBP in the cytoplasm of oocytes, and early cleavage-stage embryos. To this end, the expression of SLBP in murine oocytes and pre-implantation embryos was characterized by RT-PCR, Western blotting, and immunocytochemical. techniques. / mRNA encoding SLBP was detected throughout oogenesis and pre-implantation development, from small growing oocytes to the late blastocyst stage. SLBP protein was found in the nucleus and cytoplasm of growing and fully-gown prophase I-arrested oocytes. SLBP accumulated to extremely high levels during meiotic maturation in a process requiring translation. The protein remained abundant both in the nucleus and cytoplasm throughout the 1- and 2-cell stages. SLBP was depleted in 4-cell embryos in a process independent of DNA replication, and was not detected again until the late 8-cell stage. From the late 8-cell stage to the early blastocyst stage, SLBP was detected exclusively in the cytoplasm. Interestingly, in late blastocysts, SLBP was translocated to the nucleus. (Abstract shortened by UMI.)
188

Human centromeric and neocentromeric chromatin

Lo, Wing Ip Anthony Unknown Date (has links)
Human centromeres contain large arrays of α-satellite DNA that are thought to provide centromere function. These arrays show size and sequence variations. However, the lower limit of the sizes of these DNA arrays in normal centromeres is unknown. Using a set of chromosome-specific α-satellite probes for each of the human chromosomes, interphase Fluorescence In Situ Hybridisation (FISH) was performed in a population screening study. This study demonstrated that extreme reduction of chromosome-specific α-satellite is unusually common in chromosome 21 (screened with the αRI probe), with a prevalence of 3.70%, compared to <=.12 % for each of chromosomes 13 and 17, and 0 % for the other chromosomes. No analphoid centromere was identified in over 17,000 morphologically normal chromosomes studied. All the low-alphoid centromeres are fully functional as indicated by their mitotic stability and binding to centromere proteins including CENtromere Protein-A (CENP-A), CENtromere Protein-B (CENP-B), CENtromere Protein-C (CENP-C), and CENtromere Protein-E (CENP-E). Sensitive metaphase FISH analysis of the low-alphoid chromosome 21 centromeres established the presence of residual αRI as well as other non-αRI α-satellite DNA suggesting that centromere function may be provided by (i) the residual αRI DNA, (ii) other non-αRI a-satellite sequences, (iii) a combination of i and ii, or (iv) an activated neocentromere DNA. (For complete abstract open document)
189

In vivo Analyse der Chromatinstruktur ribosomaler DNA in S. cerevisiae

Merz, Katharina January 2007 (has links)
Regensburg, Univ., Diss., 2007
190

The Role of Chromatin Remodeling in Hippocampus in Depression and Antidepressant Action

Tsankova, Nadejda Mincheva January 2008 (has links)
Dissertation (Ph.D.) -- University of Texas Southwestern Medical Center at Dallas, 2008. / Vita. Bibliography: p.184-192

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